##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AclI AgeI AhdI Alw44I
AocI ApaLI AscI AseI AsnI
AspEI AspI AviII BalI BciVI
BclI BglII BlpI Bpu1102I BsaBI
BsaI BsiWI BspDI BspHI BspLU11I
BsrDI BstEII BstPI Bsu36I CelII
ClaI Eam1105I Eco81I EcoNI EcoO65I
EcoRI EcoRV FbaI FseI FspI
HincII HindII HpaI MamI MfeI
MluI MluNI MscI MunI NcoI
NheI NruI PacI PflFI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI SalI SbfI ScaI
SgrAI SnaBI SpeI SphI Sse8387I
SwaI Tth111I Van91I
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 1689 GGTACC G^GTACC
AccI 3126 GTATAC GT^[AC][GT]AC
AccIII 1273 TCCGGA T^CCGGA
AflII 1827 CTTAAG C^TTAAG
AflIII 3470 ACGTGT A^C[GA][CT]GT
Aor51HI 14 AGCGCT AGC^GCT
ApaI 751 GGGCCC GGGCC^C
Asp700I 38 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1689 GGTACC G^GTACC
AvrII 3088 CCTAGG C^CTAGG
BbrPI 3266 CACGTG CAC^GTG
BbvCI 669 CCTCAGC CCTCAGC(-5/-2)
BcgI 85 GCATTTGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1827 CTTAAG C^TTAAG
BlnI 3088 CCTAGG C^CTAGG
BseAI 1273 TCCGGA T^CCGGA
BsiEI 213 CGGCCG CG[GA][CT]^CG
BsmBI 1449 GAGACG CGTCTC(1/5)
BspEI 1273 TCCGGA T^CCGGA
BssHII 743 GCGCGC G^CGCGC
Bst1107I 3126 GTATAC GTA^TAC
BstAPI 1786 GCAGCATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1941 TTCGAA TT^CGAA
BstXI 1091 CCATCGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3126 GTATAC GTA^TAC
CpoI 501 CGGTCCG CG^G[AT]CCG
DrdI 1157 GACTAGAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 213 CGGCCG C^GGCCG
EclXI 213 CGGCCG C^GGCCG
Eco47III 14 AGCGCT AGC^GCT
Eco52I 213 CGGCCG C^GGCCG
EcoT22I 3106 ATGCAT ATGCA^T
HindIII 1111 AAGCTT A^AGCTT
KpnI 1689 GGTACC GGTAC^C
KspI 540 CCGCGG CCGC^GG
MroI 1273 TCCGGA T^CCGGA
NdeI 1790 CATATG CA^TATG
NotI 212 GCGGCCGC GC^GGCCGC
NsiI 3106 ATGCAT ATGCA^T
NspV 1941 TTCGAA TT^CGAA
PaeR7I 264 CTCGAG C^TCGAG
PmaCI 3266 CACGTG CAC^GTG
PmlI 3266 CACGTG CAC^GTG
PvuII 898 CAGCTG CAG^CTG
RsrII 501 CGGTCCG CG^G[AT]CCG
SacI 2358 GAGCTC GAGCT^C
SacII 540 CCGCGG CCGC^GG
SexAI 3529 ACCTGGT A^CC[AT]GGT
SfiI 471 GGCCACCCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1941 TTCGAA TT^CGAA
XbaI 1167 TCTAGA T^CTAGA
XcmI 584 CCAAACGCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 264 CTCGAG C^TCGAG
XmnI 38 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwNI 2873 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
2980 CAGTAACTG
BamHI 22 GGATCC G^GATCC
447 GGATCC
BbeI 526 GGCGCC GGCGC^C
1741 GGCGCC
BglI 472 GCCACCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
674 GCCCCCCCGGC
BseRI 1746 CTCCTC GAGGAG(10/8)
1138 GAGGAG
BsgI 1492 CTGCAC GTGCAG(16/14)
2240 CTGCAC
BspMI 773 ACCTGC ACCTGC(4/8)
3249 ACCTGC
BsrBI 713 CCGCTC CCGCTC(-3/-3)
522 GAGCGG
BssSI 351 CTCGTG CACGAG(-5/-1)
3361 CTCGTG
DraI 2379 TTTAAA TTT^AAA
2783 TTTAAA
DraIII 1907 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
2001 CACAGGGTG
DsaI 540 CCGCGG C^C[GA][CT]GG
1133 CCACGG
KasI 526 GGCGCC G^GCGCC
1741 GGCGCC
NarI 526 GGCGCC GG^CGCC
1741 GGCGCC
PstI 690 CTGCAG CTGCA^G
2667 CTGCAG
SapI 555 GCTCTTC GCTCTTC(1/4)
1044 GCTCTTC
SfcI 690 CTGCAG C^T[GA][CT]AG
2667 CTGCAG
SfoI 526 GGCGCC GGC^GCC
1741 GGCGCC
SspI 2692 AATATT AAT^ATT
3292 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaAI BsaHI BsaJI BsaWI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BsrFI BsrGI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NspI PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SmaI SmlI SspBI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmaCI XmaI