##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AccII AccIII AflII
AgeI AhdI Aor51HI ApaI AscI
Asp718I AspEI AviII AvrII BalI
BamHI BciVI BfrI BglI BglII
BlnI BlpI Bpu1102I BseAI BsiEI
BsmBI BspDI BspEI BspLU11I BsrBI
BssHII Bst1107I BstEII BstPI BstUI
BstZ17I CelII ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I FauI FseI FspI KpnI
KspI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NdeI
NgoMIV NheI NotI NruI PacI
PaeR7I PinAI PmeI PvuI RsrII
SacII SalI SbfI SexAI SfiI
SgrAI SmaI SnaBI SphI Sse8387I
SspI SwaI XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AccI 129 GTCTAC GT^[AC][GT]AC
AclI 1953 AACGTT AA^CGTT
AflIII 3496 ACGTGT A^C[GA][CT]GT
Alw44I 2651 GTGCAC G^TGCAC
AlwNI 584 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 2651 GTGCAC G^TGCAC
AseI 1857 ATTAAT AT^TAAT
AsnI 1857 ATTAAT AT^TAAT
AspI 2436 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1069 GGCGCC GGCGC^C
BbrPI 1572 CACGTG CAC^GTG
BcgI 1029 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4061 TGATCA T^GATCA
BcnI 3831 CCGGG CC^[GC]GG
BsaAI 1572 CACGTG [CT]AC^GT[GA]
BsaBI 739 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1595 ACCGGA [AT]^CCGG[AT]
BsiWI 2980 CGTACG C^GTACG
BspHI 1862 TCATGA T^CATGA
BsrDI 3656 CATTGC GCAATG(2/0)
BsrFI 10 GCCGGT [GA]^CCGG[CT]
BssSI 3614 CACGAG CACGAG(-5/-1)
BstAPI 3157 GCAATCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2394 TTCGAA TT^CGAA
Cfr10I 10 GCCGGT [GA]^CCGG[CT]
DraI 975 TTTAAA TTT^AAA
DsaI 2263 CCATGG C^C[GA][CT]GG
EaeI 8 TGGCCG [CT]^GGCC[GA]
EcoNI 3175 CCTTTGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 850 GATATC GAT^ATC
EcoT14I 2263 CCATGG C^C[AT][AT]GG
FbaI 4061 TGATCA T^GATCA
HaeII 1069 GGCGCC [GA]GCGC^[CT]
HpaI 3735 GTTAAC GTT^AAC
KasI 1069 GGCGCC G^GCGCC
MamI 739 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1069 GGCGCC GG^CGCC
NciI 3831 CCGGG CC^[GC]GG
NcoI 2263 CCATGG C^CATGG
NspI 3467 GCATGT [GA]CATG^[CT]
NspV 2394 TTCGAA TT^CGAA
PflFI 2436 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1568 CCACCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1572 CACGTG CAC^GTG
PmlI 1572 CACGTG CAC^GTG
PshAI 3464 GACGCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1857 ATTAAT AT^TAAT
Psp1406I 1953 AACGTT AA^CGTT
PstI 1064 CTGCAG CTGCA^G
RcaI 1862 TCATGA T^CATGA
SapI 3479 GAAGAGC GCTCTTC(1/4)
ScaI 207 AGTACT AGT^ACT
SfoI 1069 GGCGCC GGC^GCC
SfuI 2394 TTCGAA TT^CGAA
SpeI 1270 ACTAGT A^CTAGT
StuI 149 AGGCCT AGG^CCT
StyI 2263 CCATGG C^C[AT][AT]GG
TfiI 1030 GATTC G^A[AT]TC
Tth111I 2436 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1568 CCACCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 66 GACGCC G[GA]^CG[CT]C
1069 GGCGCC
AocI 1160 CCTCAGG CC^T[ACGT]AGG
3399 CCTTAGG
AvaI 493 CCCGAG C^[CT]CG[GA]G
1049 CCCGAG
BbvCI 1178 CCTCAGC CCTCAGC(-5/-2)
4009 GCTGAGG
BsaHI 66 GACGCC G[GA]^CG[CT]C
1069 GGCGCC
BsmFI 1267 GGGAC GGGAC(10/14)
1015 GTCCC
BsoBI 493 CCCGAG C^[CT]CG[GA]G
1049 CCCGAG
BstXI 661 CCAACCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1188 CCAAAGTTCTGG
BstYI 568 AGATCC [GA]^GATC[CT]
2514 AGATCC
Bsu36I 1160 CCTCAGG CC^T[ACGT]AGG
3399 CCTTAGG
CfoI 1070 GCGC GCG^C
3978 GCGC
DraIII 1569 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
2097 CACAGTGTG
Eco81I 1160 CCTCAGG CC^T[ACGT]AGG
3399 CCTTAGG
EcoT22I 3201 ATGCAT ATGCA^T
3565 ATGCAT
HgaI 66 GACGC GACGC(5/10)
3464 GACGC
HhaI 1070 GCGC GCG^C
3978 GCGC
Hin1I 66 GACGCC G[GA]^CG[CT]C
1069 GGCGCC
HinP1I 1070 GCGC G^CGC
3978 GCGC
HindIII 3424 AAGCTT A^AGCTT
3751 AAGCTT
MflI 568 AGATCC [GA]^GATC[CT]
2514 AGATCC
NsiI 3201 ATGCAT ATGCA^T
3565 ATGCAT
PpuMI 243 AGGACCT [GA]G^G[AT]CC[CT]
463 AGGACCT
PvuII 377 CAGCTG CAG^CTG
2345 CAGCTG
SacI 3185 GAGCTC GAGCT^C
3269 GAGCTC
XcmI 31 CCAAGAGCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
658 CCACCAACCTGTTGG
XhoII 568 AGATCC [GA]^GATC[CT]
2514 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AvaII BanI
BanII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmI Bsp1286I Bsp1407I BspMI BsrGI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRI EcoRII Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XmnI