##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AhdI Aor51HI ApaI ApoI AscI
Asp718I AspEI BclI BfrI BsaBI
BsiWI BspDI BspLU11I BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
DraI DrdI Eam1105I Eco47III EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HindIII HpaI KpnI MamI MfeI
MluI MunI NdeI NheI NotI
NruI NsiI NspV PacI PmeI
Psp1406I PvuI SalI SapI SbfI
ScaI SfuI SnaBI SpeI Sse8387I
SspI SwaI Tsp509I XbaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 1892 GTCTAC GT^[AC][GT]AC
AccIII 517 TCCGGA T^CCGGA
AflIII 2212 ACGTGT A^C[GA][CT]GT
AgeI 1520 ACCGGT A^CCGGT
Alw44I 2969 GTGCAC G^TGCAC
ApaLI 2969 GTGCAC G^TGCAC
AseI 3047 ATTAAT AT^TAAT
AsnI 3047 ATTAAT AT^TAAT
Asp700I 2518 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2651 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2217 TGCGCA TGC^GCA
AvrII 3388 CCTAGG C^CTAGG
BamHI 2127 GGATCC G^GATCC
BbrPI 2211 CACGTG CAC^GTG
BbsI 3061 GAAGAC GAAGAC(2/6)
BbvCI 2393 GCTGAGG CCTCAGC(-5/-2)
BlnI 3388 CCTAGG C^CTAGG
BpuAI 3061 GAAGAC GAAGAC(2/6)
BseAI 517 TCCGGA T^CCGGA
BsgI 2228 GTGCAG GTGCAG(16/14)
BsmBI 171 CGTCTC CGTCTC(1/5)
BsmI 3683 GCATTC GAATGC(1/-1)
Bsp1407I 1719 TGTACA T^GTACA
BspEI 517 TCCGGA T^CCGGA
BsrGI 1719 TGTACA T^GTACA
BssSI 2040 CACGAG CACGAG(-5/-1)
CpoI 2819 CGGACCG CG^G[AT]CCG
EcoNI 2224 CCTGGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 2217 TGCGCA TGC^GCA
MroI 517 TCCGGA T^CCGGA
NaeI 1503 GCCGGC GCC^GGC
NgoMIV 1503 GCCGGC G^CCGGC
PaeR7I 371 CTCGAG C^TCGAG
PflFI 2651 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1480 CCACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1520 ACCGGT A^CCGGT
PmaCI 2211 CACGTG CAC^GTG
PmlI 2211 CACGTG CAC^GTG
PshAI 3802 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3047 ATTAAT AT^TAAT
RsrII 2819 CGGACCG CG^G[AT]CCG
SexAI 2247 ACCTGGT A^CC[AT]GGT
SfiI 256 GGCCATGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 2776 CACCGGCG C[GA]^CCGG[CT]G
SphI 1854 GCATGC GCATG^C
SspBI 1719 TGTACA T^GTACA
StuI 2044 AGGCCT AGG^CCT
Tth111I 2651 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1480 CCACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 371 CTCGAG C^TCGAG
XmnI 2518 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AocI 665 CCTAAGG CC^T[ACGT]AGG
1079 CCTGAGG
BcgI 1564 CGAGGACGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2784 GCATGGGCATCG
BciVI 2477 GTATCC GTATCC(6/5)
2564 GTATCC
BglI 38 GCCCCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
257 GCCATGGCGGC
BglII 2052 AGATCT A^GATCT
2067 AGATCT
BlpI 286 GCTCAGC GC^T[ACGT]AGC
1241 GCTGAGC
Bpu1102I 286 GCTCAGC GC^T[ACGT]AGC
1241 GCTGAGC
BsaAI 1175 TACGTG [CT]AC^GT[GA]
2211 CACGTG
BspHI 1977 TCATGA T^CATGA
2902 TCATGA
BsrBI 1058 GAGCGG CCGCTC(-3/-3)
2555 GAGCGG
BstAPI 334 GCAGACGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1845 GCAGCCTGTGC
Bsu36I 665 CCTAAGG CC^T[ACGT]AGG
1079 CCTGAGG
CelII 286 GCTCAGC GC^T[ACGT]AGC
1241 GCTGAGC
Eco81I 665 CCTAAGG CC^T[ACGT]AGG
1079 CCTGAGG
HincII 2795 GTCAAC GT[CT]^[GA]AC
3164 GTTGAC
HindII 2795 GTCAAC GT[CT]^[GA]AC
3164 GTTGAC
MseI 2982 TTAA T^TAA
3048 TTAA
PstI 3311 CTGCAG CTGCA^G
3970 CTGCAG
RcaI 1977 TCATGA T^CATGA
2902 TCATGA
Tru9I 2982 TTAA T^TAA
3048 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BalI BanI BanII BbeI BbvI
BcnI BfaI BmyI BpmI BsaHI
BsaI BsaJI BsaWI BseRI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EagI EarI EclXI
Eco52I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PvuII RsaI SacI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I XcmI
XhoII XmaCI XmaI