##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AccI AccIII AflII AgeI AscI
AseI AsnI AspI AviII AvrII
BamHI BciVI BfrI BlnI BlpI
Bpu1102I BsaWI BseAI BsiWI BspDI
BspEI BspHI BspLU11I Bst1107I BstBI
BstZ17I CelII ClaI DraIII DrdI
EagI EclXI Eco52I EcoRI EcoRV
FseI FspI HindIII KspI MfeI
MluI MroI MunI NdeI NotI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI PvuI RcaI
SacII SalI SexAI SfiI SfuI
SgrAI SpeI SwaI Tth111I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 101 GACGTC GACGT^C
Acc65I 1964 GGTACC G^GTACC
AclI 3260 AACGTT AA^CGTT
AflIII 2824 ACACGT A^C[GA][CT]GT
AhdI 3563 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3977 GTGCAC G^TGCAC
AlwNI 2852 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 3977 GTGCAC G^TGCAC
Asp700I 680 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1964 GGTACC G^GTACC
AspEI 3563 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 3444 CACGTG CAC^GTG
BclI 796 TGATCA T^GATCA
BglII 3154 AGATCT A^GATCT
BsaBI 560 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 228 GAGGAG GAGGAG(10/8)
BsiEI 72 CGACCG CG[GA][CT]^CG
Bsp1407I 2765 TGTACA T^GTACA
BsrGI 2765 TGTACA T^GTACA
BssHII 180 GCGCGC G^CGCGC
BssSI 338 CTCGTG CACGAG(-5/-1)
BstEII 1001 GGTGACC G^GT[ACGT]ACC
BstPI 1001 GGTGACC G^GT[ACGT]ACC
CpoI 1104 CGGACCG CG^G[AT]CCG
Eam1105I 3563 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1001 GGTGACC G^GT[ACGT]ACC
FbaI 796 TGATCA T^GATCA
HpaI 2105 GTTAAC GTT^AAC
KpnI 1964 GGTACC GGTAC^C
MamI 560 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 639 GCCGGC GCC^GGC
NgoMIV 639 GCCGGC G^CCGGC
NheI 3998 GCTAGC G^CTAGC
NruI 105 TCGCGA TCG^CGA
PmaCI 3444 CACGTG CAC^GTG
PmlI 3444 CACGTG CAC^GTG
PpuMI 81 AGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 3260 AACGTT AA^CGTT
PvuII 4004 CAGCTG CAG^CTG
RsrII 1104 CGGACCG CG^G[AT]CCG
SacI 3718 GAGCTC GAGCT^C
SbfI 1180 CCTGCAGG CCTGCA^GG
ScaI 3268 AGTACT AGT^ACT
SmlI 1583 CTCAAG C^T[CT][GA]AG
SnaBI 2790 TACGTA TAC^GTA
SphI 1558 GCATGC GCATG^C
Sse8387I 1180 CCTGCAGG CCTGCA^GG
SspBI 2765 TGTACA T^GTACA
SspI 3848 AATATT AAT^ATT
XcmI 2247 CCAATTATGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 680 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AocI 713 CCTCAGG CC^T[ACGT]AGG
3357 CCTGAGG
Aor51HI 110 AGCGCT AGC^GCT
3931 AGCGCT
BcgI 526 GCAGCTTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
724 GCAGTATGTTCG
BsmBI 399 CGTCTC CGTCTC(1/5)
1516 CGTCTC
BsrBI 780 GAGCGG CCGCTC(-3/-3)
1083 GAGCGG
BsrDI 866 CATTGC GCAATG(2/0)
965 CATTGC
BstAPI 267 GCACGCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2640 GCAGTTTATGC
BstYI 1498 GGATCT [GA]^GATC[CT]
3154 AGATCT
Bsu36I 713 CCTCAGG CC^T[ACGT]AGG
3357 CCTGAGG
DraI 2116 TTTAAA TTT^AAA
2873 TTTAAA
Eco47III 110 AGCGCT AGC^GCT
3931 AGCGCT
Eco81I 713 CCTCAGG CC^T[ACGT]AGG
3357 CCTGAGG
HincII 657 GTCAAC GT[CT]^[GA]AC
2105 GTTAAC
HindII 657 GTCAAC GT[CT]^[GA]AC
2105 GTTAAC
MflI 1498 GGATCT [GA]^GATC[CT]
3154 AGATCT
MspA1I 3534 CCGCTG C[AC]G^C[GT]G
4004 CAGCTG
NcoI 1090 CCATGG C^CATGG
3091 CCATGG
SmaI 92 CCCGGG CCC^GGG
3498 CCCGGG
StuI 478 AGGCCT AGG^CCT
807 AGGCCT
XhoII 1498 GGATCT [GA]^GATC[CT]
3154 AGATCT
XmaCI 92 CCCGGG C^CCGGG
3498 CCCGGG
XmaI 92 CCCGGG C^CCGGG
3498 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApaI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbsI BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV NsiI NspI PflMI PleI
PstI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I