##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII AccII AccIII AgeI ApaI
AscI AvaI AviII AvrII BamHI
BbrPI BcgI BclI BcnI BglI
BlnI BsaWI BseAI BsiEI BsiWI
BsoBI BspDI BspEI BspMI BssHII
BssSI Bst1107I BstBI BstUI BstXI
BstZ17I ClaI CpoI DrdI EagI
EclXI Eco52I FbaI FseI FspI
HgaI KspI MfeI MluI MroI
MunI MvnI NciI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflMI PinAI PmaCI PmlI PshAI
PvuI RsrII SacII SapI SbfI
SfiI SfuI SgrAI SmaI SnaBI
SphI Sse8387I StuI SwaI Van91I
XbaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 1105 GGTACC G^GTACC
AccI 3805 GTCGAC GT^[AC][GT]AC
AclI 4229 AACGTT AA^CGTT
AcyI 167 GGCGCC G[GA]^CG[CT]C
AflII 3310 CTTAAG C^TTAAG
AhdI 3639 GACATCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1318 GTGCAC G^TGCAC
AlwNI 1873 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 26 AGCGCT AGC^GCT
ApaLI 1318 GTGCAC G^TGCAC
Asp718I 1105 GGTACC G^GTACC
AspEI 3639 GACATCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3799 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 3689 GAGCTC G[GA]GC[CT]^C
BbeI 167 GGCGCC GGCGC^C
BbvCI 73 GCTGAGG CCTCAGC(-5/-2)
BfrI 3310 CTTAAG C^TTAAG
BlpI 259 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 259 GCTTAGC GC^T[ACGT]AGC
BsaHI 167 GGCGCC G[GA]^CG[CT]C
BsaI 2012 GGTCTC GGTCTC(1/5)
BsmBI 174 GAGACG CGTCTC(1/5)
BsrBI 43 GAGCGG CCGCTC(-3/-3)
BsrDI 1632 GCAATG GCAATG(2/0)
BsrFI 164 GCCGGC [GA]^CCGG[CT]
BstAPI 2622 GCAACAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 259 GCTTAGC GC^T[ACGT]AGC
Cfr10I 164 GCCGGC [GA]^CCGG[CT]
DraIII 1481 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 3374 CCATGG C^C[GA][CT]GG
Eam1105I 3639 GACATCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 26 AGCGCT AGC^GCT
EcoNI 2112 CCTATAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2387 GAATTC G^AATTC
Hin1I 167 GGCGCC G[GA]^CG[CT]C
HpaI 842 GTTAAC GTT^AAC
KasI 167 GGCGCC G^GCGCC
KpnI 1105 GGTACC GGTAC^C
NaeI 164 GCCGGC GCC^GGC
NarI 167 GGCGCC GG^CGCC
NcoI 3374 CCATGG C^CATGG
NgoMIV 164 GCCGGC G^CCGGC
PflFI 3799 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmeI 1199 GTTTAAAC GTTT^AAAC
PpuMI 1078 GGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 4229 AACGTT AA^CGTT
PvuII 1861 CAGCTG CAG^CTG
SacI 3689 GAGCTC GAGCT^C
SalI 3805 GTCGAC G^TCGAC
ScaI 2601 AGTACT AGT^ACT
SexAI 388 ACCTGGT A^CC[AT]GGT
SfoI 167 GGCGCC GGC^GCC
SpeI 1794 ACTAGT A^CTAGT
Tth111I 3799 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AocI 2424 CCTCAGG CC^T[ACGT]AGG
3962 CCTTAGG
AseI 3927 ATTAAT AT^TAAT
3975 ATTAAT
AsnI 3927 ATTAAT AT^TAAT
3975 ATTAAT
BalI 1020 TGGCCA TGG^CCA
3436 TGGCCA
BciVI 403 GGATAC GTATCC(6/5)
2890 GGATAC
BglII 2058 AGATCT A^GATCT
3736 AGATCT
BsaAI 1154 CACGTA [CT]AC^GT[GA]
3597 TACGTG
BsaBI 720 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3421 GATATCTATC
BseRI 19 GAGGAG GAGGAG(10/8)
1194 GAGGAG
BsgI 4243 CTGCAC GTGCAG(16/14)
2900 GTGCAG
BspHI 3054 TCATGA T^CATGA
4360 TCATGA
BspLU11I 545 ACATGT A^CATGT
3869 ACATGT
BstEII 794 GGTAACC G^GT[ACGT]ACC
2536 GGTGACC
BstPI 794 GGTAACC G^GT[ACGT]ACC
2536 GGTGACC
Bsu36I 2424 CCTCAGG CC^T[ACGT]AGG
3962 CCTTAGG
DraII 81 AGGCCCC [GA]G^G[ACGT]CC[CT]
1078 GGGACCC
EaeI 1020 TGGCCA [CT]^GGCC[GA]
3436 TGGCCA
Eco81I 2424 CCTCAGG CC^T[ACGT]AGG
3962 CCTTAGG
EcoO109I 81 AGGCCCC [GA]G^G[ACGT]CC[CT]
1078 GGGACCC
EcoO65I 794 GGTAACC G^GT[ACGT]ACC
2536 GGTGACC
EcoRV 2346 GATATC GAT^ATC
3421 GATATC
HaeII 26 AGCGCT [GA]GCGC^[CT]
167 GGCGCC
HapII 165 CCGG C^CGG
171 CCGG
HpaII 165 CCGG C^CGG
171 CCGG
MamI 720 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3421 GATATCTATC
MluNI 1020 TGGCCA TGG^CCA
3436 TGGCCA
MscI 1020 TGGCCA TGG^CCA
3436 TGGCCA
MslI 1775 CATGTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2784 CACTTTTATG
MspI 165 CCGG C^CGG
171 CCGG
PleI 2122 GAGTC GAGTC(4/5)
2156 GAGTC
PshBI 3927 ATTAAT AT^TAAT
3975 ATTAAT
PstI 351 CTGCAG CTGCA^G
702 CTGCAG
RcaI 3054 TCATGA T^CATGA
4360 TCATGA
SspI 3131 AATATT AAT^ATT
4023 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI Asp700I AspHI AvaII
BanI BbsI BbvI BfaI BmyI
BpmI BpuAI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BsrGI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI EarI EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeIII HhaI HinP1I HincII HindII
HindIII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NsiI
NspI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI