##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AclI AflII Aor51HI AscI AviII
BbrPI BclI BfrI BlpI Bpu1102I
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BsrGI BssHII BssSI Bst1107I
BstBI BstZ17I CelII ClaI CpoI
DraI EagI EclXI Eco47III Eco52I
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HpaI KspI MfeI
MluI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacI SacII SalI
SapI ScaI SfuI SnaBI SpeI
SphI SspBI SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 640 GACGTC GACGT^C
Acc65I 2816 GGTACC G^GTACC
AccI 2674 GTCTAC GT^[AC][GT]AC
AccII 1612 CGCG CG^CG
AcsI 2023 GAATTC [GA]^AATT[CT]
AgeI 1868 ACCGGT A^CCGGT
AhdI 1396 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 2023 GAATTC [GA]^AATT[CT]
AseI 2272 ATTAAT AT^TAAT
AsnI 2272 ATTAAT AT^TAAT
Asp700I 942 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2816 GGTACC G^GTACC
AspEI 1396 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3415 CCTAGG C^CTAGG
BciVI 1950 GGATAC GTATCC(6/5)
BglII 3594 AGATCT A^GATCT
BlnI 3415 CCTAGG C^CTAGG
BsaBI 1185 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 3258 GTGCAG GTGCAG(16/14)
BspLU11I 3235 ACATGT A^CATGT
BstUI 1612 CGCG CG^CG
DraIII 691 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1017 GACAGCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1396 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2023 GAATTC G^AATTC
HindIII 2944 AAGCTT A^AGCTT
KpnI 2816 GGTACC GGTAC^C
MamI 1185 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 1612 CGCG CG^CG
PinAI 1868 ACCGGT A^CCGGT
PleI 2217 GACTC GAGTC(4/5)
PshBI 2272 ATTAAT AT^TAAT
SbfI 1848 CCTGCAGG CCTGCA^GG
SfiI 969 GGCCATGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 1338 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 3089 CCCGGG CCC^GGG
Sse8387I 1848 CCTGCAGG CCTGCA^GG
StuI 3418 AGGCCT AGG^CCT
XbaI 3638 TCTAGA T^CTAGA
XmaCI 3089 CCCGGG C^CCGGG
XmaI 3089 CCCGGG C^CCGGG
XmnI 942 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccIII 1187 TCCGGA T^CCGGA
2849 TCCGGA
AflIII 2033 ACGTGT A^C[GA][CT]GT
3235 ACATGT
Alw44I 357 GTGCAC G^TGCAC
778 GTGCAC
AocI 254 CCTGAGG CC^T[ACGT]AGG
1737 CCTCAGG
ApaLI 357 GTGCAC G^TGCAC
778 GTGCAC
AspI 2920 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3309 GACAAAGTC
BamHI 332 GGATCC G^GATCC
2804 GGATCC
BbeI 1342 GGCGCC GGCGC^C
1726 GGCGCC
BbsI 1066 GAAGAC GAAGAC(2/6)
3114 GAAGAC
BbvCI 446 CCTCAGC CCTCAGC(-5/-2)
678 CCTCAGC
BglI 970 GCCATGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1210 GCCATTGCGGC
BpuAI 1066 GAAGAC GAAGAC(2/6)
3114 GAAGAC
BseAI 1187 TCCGGA T^CCGGA
2849 TCCGGA
BspEI 1187 TCCGGA T^CCGGA
2849 TCCGGA
BspHI 1449 TCATGA T^CATGA
1904 TCATGA
BsrBI 2418 CCGCTC CCGCTC(-3/-3)
1426 GAGCGG
BstAPI 1621 GCAGTGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2561 GCACCGCCTGC
BstEII 1365 GGTGACC G^GT[ACGT]ACC
1482 GGTGACC
BstPI 1365 GGTGACC G^GT[ACGT]ACC
1482 GGTGACC
Bsu36I 254 CCTGAGG CC^T[ACGT]AGG
1737 CCTCAGG
Eco81I 254 CCTGAGG CC^T[ACGT]AGG
1737 CCTCAGG
EcoO65I 1365 GGTGACC G^GT[ACGT]ACC
1482 GGTGACC
KasI 1342 GGCGCC G^GCGCC
1726 GGCGCC
MroI 1187 TCCGGA T^CCGGA
2849 TCCGGA
NarI 1342 GGCGCC GG^CGCC
1726 GGCGCC
NcoI 287 CCATGG C^CATGG
1346 CCATGG
PflFI 2920 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3309 GACAAAGTC
PvuII 2221 CAGCTG CAG^CTG
3037 CAGCTG
RcaI 1449 TCATGA T^CATGA
1904 TCATGA
SexAI 770 ACCTGGT A^CC[AT]GGT
1478 ACCTGGT
SfoI 1342 GGCGCC GGC^GCC
1726 GGCGCC
SmlI 784 CTCAAG C^T[CT][GA]AG
3303 CTCAAG
Tsp509I 170 AATT ^AATT
2024 AATT
Tth111I 2920 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3309 GACAAAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BalI BanI BanII BbvI BcgI
BcnI BfaI BmyI BpmI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I TspRI TthHB8I Van91I
XcmI XhoII