##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
Acc65I AclI AflII AgeI Aor51HI
AscI Asp700I Asp718I AspI AviII
BamHI BbrPI BfrI BlpI Bpu1102I
BsaAI BsaBI BsiWI Bsp1407I BspDI
BspHI BspMI BsrGI Bst1107I BstBI
BstZ17I CelII ClaI CpoI Eco47III
EcoRI EcoT22I FseI FspI HincII
HindII HpaI KpnI MamI MluI
NdeI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmlI Psp1406I PvuI RcaI RsrII
SalI SbfI SexAI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SwaI Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 1355 GACGTC GACGT^C
AccI 1999 GTAGAC GT^[AC][GT]AC
Alw44I 2648 GTGCAC G^TGCAC
AocI 2193 CCTGAGG CC^T[ACGT]AGG
ApaLI 2648 GTGCAC G^TGCAC
AseI 4011 ATTAAT AT^TAAT
AsnI 4011 ATTAAT AT^TAAT
AvrII 994 CCTAGG C^CTAGG
BbeI 2887 GGCGCC GGCGC^C
BbvCI 669 CCTCAGC CCTCAGC(-5/-2)
BcgI 1682 GCAATGATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3552 GGATAC GTATCC(6/5)
BglII 1076 AGATCT A^GATCT
BlnI 994 CCTAGG C^CTAGG
BstAPI 3406 GCACAGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1848 GGTGACC G^GT[ACGT]ACC
BstPI 1848 GGTGACC G^GT[ACGT]ACC
Bsu36I 2193 CCTGAGG CC^T[ACGT]AGG
DraI 4388 TTTAAA TTT^AAA
DraIII 2508 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 346 GACAGAGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2193 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1848 GGTGACC G^GT[ACGT]ACC
EcoRV 3445 GATATC GAT^ATC
HaeII 2887 GGCGCC [GA]GCGC^[CT]
HgaI 633 GCGTC GACGC(5/10)
KasI 2887 GGCGCC G^GCGCC
NaeI 575 GCCGGC GCC^GGC
NarI 2887 GGCGCC GG^CGCC
NgoMIV 575 GCCGGC G^CCGGC
NheI 3028 GCTAGC G^CTAGC
PflMI 3478 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmeI 4387 GTTTAAAC GTTT^AAAC
PshAI 86 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4011 ATTAAT AT^TAAT
PstI 3912 CTGCAG CTGCA^G
SapI 172 GAAGAGC GCTCTTC(1/4)
ScaI 4102 AGTACT AGT^ACT
SfoI 2887 GGCGCC GGC^GCC
SphI 2460 GCATGC GCATG^C
StuI 3695 AGGCCT AGG^CCT
Van91I 3478 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
BalI 2166 TGGCCA TGG^CCA
3636 TGGCCA
BclI 2619 TGATCA T^GATCA
2781 TGATCA
BglI 993 GCCTAGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3701 GCCTGCATGGC
BsiEI 822 CGGCCG CG[GA][CT]^CG
1003 CGGCCG
BsmBI 1353 GAGACG CGTCTC(1/5)
3774 GAGACG
BspLU11I 1178 ACATGT A^CATGT
4446 ACATGT
BssHII 72 GCGCGC G^CGCGC
727 GCGCGC
EagI 822 CGGCCG C^GGCCG
1003 CGGCCG
EarI 172 GAAGAG CTCTTC(1/4)
1048 GAAGAG
EclXI 822 CGGCCG C^GGCCG
1003 CGGCCG
Eco52I 822 CGGCCG C^GGCCG
1003 CGGCCG
EcoNI 204 CCTACAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
495 CCTGGATCAGG
FbaI 2619 TGATCA T^GATCA
2781 TGATCA
HindIII 2632 AAGCTT A^AGCTT
4165 AAGCTT
Ksp632I 172 GAAGAG CTCTTC(1/4)
1048 GAAGAG
KspI 117 CCGCGG CCGC^GG
819 CCGCGG
MfeI 287 CAATTG C^AATTG
2522 CAATTG
MluNI 2166 TGGCCA TGG^CCA
3636 TGGCCA
MscI 2166 TGGCCA TGG^CCA
3636 TGGCCA
MslI 369 CACCCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2409 CACTGGAGTG
MunI 287 CAATTG C^AATTG
2522 CAATTG
SacI 3008 GAGCTC GAGCT^C
3394 GAGCTC
SacII 117 CCGCGG CCGC^GG
819 CCGCGG
SmaI 309 CCCGGG CCC^GGG
794 CCCGGG
SspI 3348 AATATT AAT^ATT
4278 AATATT
XmaCI 309 CCCGGG C^CCGGG
794 CCCGGG
XmaI 309 CCCGGG C^CCGGG
794 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AccIII AciI AcsI AcyI
AfaI AflIII AhdI AluI AlwI
AlwNI ApaI ApoI AspEI AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspEI BsrBI BsrDI BsrFI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI Eam1105I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MroI MseI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII