##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
AviII AvrII BamHI BbeI BcgI
BlnI BlpI Bpu1102I BseAI BsiWI
BspDI BspEI BspHI BssHII BssSI
Bst1107I BstBI BstZ17I Bsu36I CelII
ClaI CpoI EagI EclXI Eco47III
Eco52I Eco81I EcoNI FseI FspI
HaeII KasI KpnI KspI MluI
MroI NarI NcoI NheI NotI
NruI NspV PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SpeI SphI Sse8387I SwaI Tth111I
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccII 1480 CGCG CG^CG
AcyI 246 GACGCC G[GA]^CG[CT]C
AflII 2885 CTTAAG C^TTAAG
AflIII 4466 ACATGT A^C[GA][CT]GT
AhdI 1719 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1719 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 3942 TGGCCA TGG^CCA
BanI 410 GGTGCC G^G[CT][GA]CC
BfrI 2885 CTTAAG C^TTAAG
BglI 2280 GCCTTACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1956 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 246 GACGCC G[GA]^CG[CT]C
BsaI 3312 GGTCTC GGTCTC(1/5)
BsaWI 3949 ACCGGA [AT]^CCGG[AT]
BsiEI 72 CGACCG CG[GA][CT]^CG
BsmBI 2214 GAGACG CGTCTC(1/5)
BspLU11I 4466 ACATGT A^CATGT
BsrBI 1209 GAGCGG CCGCTC(-3/-3)
BstEII 306 GGTGACC G^GT[ACGT]ACC
BstPI 306 GGTGACC G^GT[ACGT]ACC
BstUI 1480 CGCG CG^CG
DrdI 2456 GACTGTTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 18 CCACGG C^C[GA][CT]GG
EaeI 3942 TGGCCA [CT]^GGCC[GA]
Eam1105I 1719 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 306 GGTGACC G^GT[ACGT]ACC
EcoRI 1378 GAATTC G^AATTC
EcoRV 1794 GATATC GAT^ATC
HgaI 246 GACGC GACGC(5/10)
Hin1I 246 GACGCC G[GA]^CG[CT]C
MamI 1956 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 3942 TGGCCA TGG^CCA
MscI 3942 TGGCCA TGG^CCA
MvnI 1480 CGCG CG^CG
NaeI 175 GCCGGC GCC^GGC
NdeI 346 CATATG CA^TATG
NgoMIV 175 GCCGGC G^CCGGC
PacI 4063 TTAATTAA TTAAT^TAA
PaeR7I 2964 CTCGAG C^TCGAG
PpuMI 3096 AGGACCT [GA]G^G[AT]CC[CT]
SacI 2925 GAGCTC GAGCT^C
SalI 3499 GTCGAC G^TCGAC
SmaI 95 CCCGGG CCC^GGG
SnaBI 3331 TACGTA TAC^GTA
XcmI 2944 CCATGACCACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2964 CTCGAG C^TCGAG
XmaCI 95 CCCGGG C^CCGGG
XmaI 95 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 328 GTAGAC GT^[AC][GT]AC
3499 GTCGAC
ApaI 92 GGGCCC GGGCC^C
2902 GGGCCC
AvaI 95 CCCGGG C^[CT]CG[GA]G
2964 CTCGAG
BbrPI 2550 CACGTG CAC^GTG
3150 CACGTG
BbvCI 79 CCTCAGC CCTCAGC(-5/-2)
3100 CCTCAGC
BclI 2371 TGATCA T^GATCA
3616 TGATCA
BglII 964 AGATCT A^GATCT
3188 AGATCT
BpmI 2928 CTCCAG CTGGAG(16/14)
2998 CTGGAG
BsmI 3015 GAATGC GAATGC(1/-1)
3182 GAATGC
BsoBI 95 CCCGGG C^[CT]CG[GA]G
2964 CTCGAG
Bsp1407I 893 TGTACA T^GTACA
2399 TGTACA
BspMI 3588 GCAGGT ACCTGC(4/8)
4151 GCAGGT
BsrGI 893 TGTACA T^GTACA
2399 TGTACA
BstAPI 1194 GCATACTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3353 GCAGGAAATGC
BstXI 487 CCACCAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1103 CCAGGCCTTTGG
CfoI 484 GCGC GCG^C
861 GCGC
DraIII 143 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
672 CACCTTGTG
EcoT14I 1691 CCTTGG C^C[AT][AT]GG
3551 CCTTGG
EcoT22I 622 ATGCAT ATGCA^T
1081 ATGCAT
FbaI 2371 TGATCA T^GATCA
3616 TGATCA
HhaI 484 GCGC GCG^C
861 GCGC
HinP1I 484 GCGC G^CGC
861 GCGC
HindIII 1246 AAGCTT A^AGCTT
1546 AAGCTT
HpaI 1145 GTTAAC GTT^AAC
3631 GTTAAC
MfeI 1006 CAATTG C^AATTG
2776 CAATTG
MunI 1006 CAATTG C^AATTG
2776 CAATTG
NsiI 622 ATGCAT ATGCA^T
1081 ATGCAT
PmaCI 2550 CACGTG CAC^GTG
3150 CACGTG
PmlI 2550 CACGTG CAC^GTG
3150 CACGTG
PstI 85 CTGCAG CTGCA^G
607 CTGCAG
SspBI 893 TGTACA T^GTACA
2399 TGTACA
StuI 1105 AGGCCT AGG^CCT
4274 AGGCCT
StyI 1691 CCTTGG C^C[AT][AT]GG
3551 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BanII
BbsI BbvI BciVI BcnI BfaI
BmyI BpuAI BsaAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr10I Cfr13I DdeI DpnII DraI
DraII EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII