##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Aor51HI AscI AseI AsnI
Asp718I AspEI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BfrI BglI BlnI BsaI
BsiEI BsiWI BsmBI BspDI BssHII
BssSI BstBI BstEII BstPI ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV FseI FspI HaeII KasI
KpnI MluI MluNI MscI NaeI
NarI NdeI NgoMIV NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacI SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SphI Sse8387I SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccIII 29 TCCGGA T^CCGGA
AcyI 270 GACGCC G[GA]^CG[CT]C
Alw44I 427 GTGCAC G^TGCAC
AocI 148 CCTGAGG CC^T[ACGT]AGG
ApaI 262 GGGCCC GGGCC^C
ApaLI 427 GTGCAC G^TGCAC
AspI 1182 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 2068 GGATAC GTATCC(6/5)
BsaAI 2395 TACGTA [CT]AC^GT[GA]
BsaBI 651 GATTAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 270 GACGCC G[GA]^CG[CT]C
BsaWI 29 TCCGGA [AT]^CCGG[AT]
BseAI 29 TCCGGA T^CCGGA
BsmAI 2881 GAGAC GTCTC(1/5)
BspEI 29 TCCGGA T^CCGGA
BspLU11I 3632 ACATGT A^CATGT
BsrBI 59 GAGCGG CCGCTC(-3/-3)
BsrFI 2329 GCCGGT [GA]^CCGG[CT]
Bst1107I 399 GTATAC GTA^TAC
BstAPI 1935 GCAAATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 299 CCAATTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 399 GTATAC GTA^TAC
Bsu36I 148 CCTGAGG CC^T[ACGT]AGG
Cfr10I 2329 GCCGGT [GA]^CCGG[CT]
EaeI 2110 TGGCCG [CT]^GGCC[GA]
Eco81I 148 CCTGAGG CC^T[ACGT]AGG
EcoT22I 1404 ATGCAT ATGCA^T
Hin1I 270 GACGCC G[GA]^CG[CT]C
HpaI 1805 GTTAAC GTT^AAC
MamI 651 GATTAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2478 CAATTG C^AATTG
MroI 29 TCCGGA T^CCGGA
MunI 2478 CAATTG C^AATTG
NheI 3115 GCTAGC G^CTAGC
NsiI 1404 ATGCAT ATGCA^T
PflFI 1182 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 3463 GGGACCT [GA]G^G[AT]CC[CT]
SmaI 188 CCCGGG CCC^GGG
SnaBI 2395 TACGTA TAC^GTA
StuI 1622 AGGCCT AGG^CCT
TaqI 1041 TCGA T^CGA
Tth111I 1182 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1041 TCGA T^CGA
XcmI 413 CCAAATTGTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 188 CCCGGG C^CCGGG
XmaI 188 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 399 GTATAC GT^[AC][GT]AC
1776 GTAGAC
AflIII 2171 ACGTGT A^C[GA][CT]GT
3632 ACATGT
AspHI 427 GTGCAC G[AT]GC[AT]^C
950 GTGCTC
BanII 248 GAGCCC G[GA]GC[CT]^C
262 GGGCCC
BbsI 701 GAAGAC GAAGAC(2/6)
1814 GAAGAC
BbvI 1791 GCAGC GCAGC(8/12)
1102 GCTGC
BclI 318 TGATCA T^GATCA
1395 TGATCA
BglII 787 AGATCT A^GATCT
2100 AGATCT
BlpI 887 GCTAAGC GC^T[ACGT]AGC
2207 GCTGAGC
Bpu1102I 887 GCTAAGC GC^T[ACGT]AGC
2207 GCTGAGC
BpuAI 701 GAAGAC GAAGAC(2/6)
1814 GAAGAC
BsiHKAI 427 GTGCAC G[AT]GC[AT]^C
950 GTGCTC
BspHI 1717 TCATGA T^CATGA
2557 TCATGA
BsrI 2042 ACTGG ACTGG(1/-1)
2459 ACTGG
CelII 887 GCTAAGC GC^T[ACGT]AGC
2207 GCTGAGC
DraIII 471 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
2913 CACATTGTG
EarI 3529 CTCTTC CTCTTC(1/4)
1903 GAAGAG
FbaI 318 TGATCA T^GATCA
1395 TGATCA
HincII 1805 GTTAAC GT[CT]^[GA]AC
2372 GTTGAC
HindII 1805 GTTAAC GT[CT]^[GA]AC
2372 GTTGAC
HindIII 2540 AAGCTT A^AGCTT
2957 AAGCTT
Ksp632I 3529 CTCTTC CTCTTC(1/4)
1903 GAAGAG
KspI 90 CCGCGG CCGC^GG
207 CCGCGG
NcoI 2767 CCATGG C^CATGG
3251 CCATGG
PstI 1831 CTGCAG CTGCA^G
1858 CTGCAG
RcaI 1717 TCATGA T^CATGA
2557 TCATGA
SacII 90 CCGCGG CCGC^GG
207 CCGCGG
SmlI 602 CTCAAG C^T[CT][GA]AG
2878 CTTGAG
SpeI 2072 ACTAGT A^CTAGT
3756 ACTAGT
TseI 1102 GCTGC G^C[AT]GC
1791 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AvaI
AvaII BcnI BfaI BmyI BpmI
BsaJI BseRI BsgI BsiYI BslI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspMI BsrDI BsrGI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SspBI
SspI StyI TfiI Tru9I Tsp45I
Tsp509I TspRI Van91I XhoII XmnI