##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AclI AflII AgeI AhdI Alw44I
AocI ApaI ApaLI AseI AsnI
AspEI AvrII BanII BbrPI BbvCI
BclI BfrI BglII BlnI BsiEI
BsiWI Bsp1407I BsrDI BsrFI BsrGI
BssSI Bst1107I BstBI BstZ17I Bsu36I
Cfr10I CpoI DraI EagI Eam1105I
EclXI Eco52I Eco81I EcoT22I FbaI
FseI HpaI KspI MfeI MluI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI SspI StuI SwaI Van91I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 3021 GTAGAC GT^[AC][GT]AC
Aor51HI 3220 AGCGCT AGC^GCT
AscI 172 GGCGCGCC GG^CGCGCC
AspHI 1956 GAGCAC G[AT]GC[AT]^C
AspI 2817 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1748 TGCGCA TGC^GCA
BbeI 22 GGCGCC GGCGC^C
BciVI 2021 GTATCC GTATCC(6/5)
BlpI 2222 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2222 GCTGAGC GC^T[ACGT]AGC
BsaAI 491 TACGTG [CT]AC^GT[GA]
BsaBI 224 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 1956 GAGCAC G[AT]GC[AT]^C
BspHI 1963 TCATGA T^CATGA
BspMI 2422 ACCTGC ACCTGC(4/8)
BssHII 173 GCGCGC G^CGCGC
BstEII 1121 GGTAACC G^GT[ACGT]ACC
BstPI 1121 GGTAACC G^GT[ACGT]ACC
CelII 2222 GCTGAGC GC^T[ACGT]AGC
DraIII 3189 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2610 GACTTGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 2067 GAAGAG CTCTTC(1/4)
Eco47III 3220 AGCGCT AGC^GCT
EcoO65I 1121 GGTAACC G^GT[ACGT]ACC
EcoRV 2625 GATATC GAT^ATC
FspI 1748 TGCGCA TGC^GCA
HgaI 84 GCGTC GACGC(5/10)
KasI 22 GGCGCC G^GCGCC
Ksp632I 2067 GAAGAG CTCTTC(1/4)
MamI 224 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 22 GGCGCC GG^CGCC
NcoI 2176 CCATGG C^CATGG
PflFI 2817 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 1836 CTGCAG CTGCA^G
RcaI 1963 TCATGA T^CATGA
SfoI 22 GGCGCC GGC^GCC
SpeI 3454 ACTAGT A^CTAGT
Tth111I 2817 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AatII 1062 GACGTC GACGT^C
1503 GACGTC
Acc65I 274 GGTACC G^GTACC
592 GGTACC
AccII 46 CGCG CG^CG
174 CGCG
AccIII 94 TCCGGA T^CCGGA
220 TCCGGA
Asp700I 1772 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3218 GAAGCGCTTC
Asp718I 274 GGTACC G^GTACC
592 GGTACC
BalI 226 TGGCCA TGG^CCA
2057 TGGCCA
BcgI 644 CGAGTACAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
979 GCATGGTGTTCG
BmyI 991 GTGCCC G[AGT]GC[ACT]^C
1956 GAGCAC
BsaI 2860 GAGACC GGTCTC(1/5)
2822 GGTCTC
BseAI 94 TCCGGA T^CCGGA
220 TCCGGA
BsmBI 1505 CGTCTC CGTCTC(1/5)
2851 CGTCTC
BsmI 1011 GAATGC GAATGC(1/-1)
2652 GAATGC
Bsp1286I 991 GTGCCC G[AGT]GC[ACT]^C
1956 GAGCAC
BspDI 2490 ATCGAT AT^CGAT
2622 ATCGAT
BspEI 94 TCCGGA T^CCGGA
220 TCCGGA
BspLU11I 2788 ACATGT A^CATGT
2905 ACATGT
BstUI 46 CGCG CG^CG
174 CGCG
BstXI 1078 CCAAGTGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2345 CCACGACTTTGG
ClaI 2490 ATCGAT AT^CGAT
2622 ATCGAT
DraII 1881 AGGTCCT [GA]G^G[ACGT]CC[CT]
2131 AGGACCT
DsaI 1213 CCGTGG C^C[GA][CT]GG
2176 CCATGG
EcoO109I 1881 AGGTCCT [GA]G^G[ACGT]CC[CT]
2131 AGGACCT
EcoRI 1134 GAATTC G^AATTC
3208 GAATTC
FauI 2762 CCCGC CCCGC(4/6)
169 GCGGG
HindIII 819 AAGCTT A^AGCTT
3475 AAGCTT
KpnI 274 GGTACC GGTAC^C
592 GGTACC
MluNI 226 TGGCCA TGG^CCA
2057 TGGCCA
MroI 94 TCCGGA T^CCGGA
220 TCCGGA
MscI 226 TGGCCA TGG^CCA
2057 TGGCCA
MvnI 46 CGCG CG^CG
174 CGCG
PpuMI 1881 AGGTCCT [GA]G^G[AT]CC[CT]
2131 AGGACCT
PvuII 1023 CAGCTG CAG^CTG
1714 CAGCTG
XmnI 1772 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3218 GAAGCGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI AlwNI ApoI AvaI
AvaII BamHI BanI BbsI BbvI
BcnI BfaI BglI BpmI BpuAI
BsaHI BsaJI BsaWI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsoBI
BsrBI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII EaeI EcoNI EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII