##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII AclI AcyI AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AspEI AspI AviII AvrII BbeI
BbvCI BfrI BlnI BlpI Bpu1102I
BsaHI BsiEI BsiWI BspDI BspHI
BsrFI BssHII CelII Cfr10I ClaI
CpoI DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoRV EcoT22I
FseI FspI HaeII HgaI Hin1I
KasI KspI MluI NaeI NarI
NgoMIV NheI NotI NruI NsiI
PacI PflFI PinAI PmeI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SwaI Tth111I XbaI XcmI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
Acc65I 1287 GGTACC G^GTACC
AccII 40 CGCG CG^CG
Asp718I 1287 GGTACC G^GTACC
AvaI 2557 CTCGAG C^[CT]CG[GA]G
BanI 1287 GGTACC G^G[CT][GA]CC
BbrPI 2474 CACGTG CAC^GTG
BcgI 1347 CGAAGATTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2895 GGATAC GTATCC(6/5)
BcnI 2054 CCCGG CC^[GC]GG
BglI 3392 GCCTTAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1509 AGATCT A^GATCT
BsaAI 2474 CACGTG [CT]AC^GT[GA]
BsaBI 1192 GATCGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 124 GAGACG CGTCTC(1/5)
BsoBI 2557 CTCGAG C^[CT]CG[GA]G
Bsp1407I 458 TGTACA T^GTACA
BspLU11I 1920 ACATGT A^CATGT
BsrBI 31 GAGCGG CCGCTC(-3/-3)
BsrGI 458 TGTACA T^GTACA
BssSI 1496 CACGAG CACGAG(-5/-1)
Bst1107I 2422 GTATAC GTA^TAC
BstAPI 3499 GCACAGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1345 TTCGAA TT^CGAA
BstEII 2657 GGTGACC G^GT[ACGT]ACC
BstPI 2657 GGTGACC G^GT[ACGT]ACC
BstUI 40 CGCG CG^CG
BstXI 3443 CCACCTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2422 GTATAC GTA^TAC
CfoI 1414 GCGC GCG^C
DraI 185 TTTAAA TTT^AAA
DrdI 917 GACTCCGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2657 GGTGACC G^GT[ACGT]ACC
HhaI 1414 GCGC GCG^C
HinP1I 1414 GCGC G^CGC
HincII 685 GTTAAC GT[CT]^[GA]AC
HindII 685 GTTAAC GT[CT]^[GA]AC
HpaI 685 GTTAAC GTT^AAC
KpnI 1287 GGTACC GGTAC^C
MamI 1192 GATCGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3763 CAATTG C^AATTG
MslI 3797 CACCTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 3763 CAATTG C^AATTG
MvnI 40 CGCG CG^CG
NciI 2054 CCCGG CC^[GC]GG
NspI 1920 ACATGT [GA]CATG^[CT]
NspV 1345 TTCGAA TT^CGAA
PaeR7I 2557 CTCGAG C^TCGAG
PflMI 2987 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2474 CACGTG CAC^GTG
PmlI 2474 CACGTG CAC^GTG
PshAI 1027 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 259 GAGCTC GAGCT^C
SfuI 1345 TTCGAA TT^CGAA
SspBI 458 TGTACA T^GTACA
Van91I 2987 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2557 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
ApaI 738 GGGCCC GGGCC^C
864 GGGCCC
AseI 209 ATTAAT AT^TAAT
2180 ATTAAT
AsnI 209 ATTAAT AT^TAAT
2180 ATTAAT
Asp700I 1196 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1519 GAAGGCCTTC
BalI 3180 TGGCCA TGG^CCA
3679 TGGCCA
BclI 282 TGATCA T^GATCA
3199 TGATCA
BsgI 1914 CTGCAC GTGCAG(16/14)
2436 GTGCAG
BsmI 727 GAATGC GAATGC(1/-1)
147 GCATTC
BsrDI 2301 CATTGC GCAATG(2/0)
3388 CATTGC
EaeI 3180 TGGCCA [CT]^GGCC[GA]
3679 TGGCCA
EcoNI 1447 CCTCATAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2934 CCTCGCTCAGG
FauI 38 CCCGC CCCGC(4/6)
1723 GCGGG
FbaI 282 TGATCA T^GATCA
3199 TGATCA
HindIII 1107 AAGCTT A^AGCTT
2599 AAGCTT
MluNI 3180 TGGCCA TGG^CCA
3679 TGGCCA
MscI 3180 TGGCCA TGG^CCA
3679 TGGCCA
NdeI 3427 CATATG CA^TATG
3810 CATATG
PpuMI 2343 AGGTCCC [GA]G^G[AT]CC[CT]
3055 AGGACCT
PshBI 209 ATTAAT AT^TAAT
2180 ATTAAT
PstI 329 CTGCAG CTGCA^G
3713 CTGCAG
PvuII 2291 CAGCTG CAG^CTG
3482 CAGCTG
SexAI 1290 ACCTGGT A^CC[AT]GGT
3312 ACCTGGT
SfcI 329 CTGCAG C^T[GA][CT]AG
3713 CTGCAG
XmnI 1196 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1519 GAAGGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccI AccIII AciI AcsI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI AspHI AvaII BamHI BanII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaI BsaJI BsaWI BseAI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspEI BspMI BsrI
BssKI BstF5I BstNI BstYI Bsu36I
Cac8I Cfr13I DdeI DpnII DraII
DsaI EarI Eco81I EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MroI MseI MspA1I
MspI MvaI MwoI NcoI NdeII
NlaIII NlaIV PleI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SmlI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII