##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII Acc65I AccI AcsI AflII
AhdI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AvrII
BamHI BclI BfrI BglII BlnI
BsiWI BsmBI Bsp1407I BspDI BspHI
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DrdI Eam1105I Eco47III EcoO65I EcoRI
EcoRV EcoT22I FbaI HindIII HpaI
KpnI MfeI MluI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PmeI PshAI PshBI
PvuI RcaI RsrII SalI SapI
SfiI SfuI SnaBI SpeI SphI
SspBI SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AclI 1830 AACGTT AA^CGTT
AflIII 965 ACATGT A^C[GA][CT]GT
AgeI 1153 ACCGGT A^CCGGT
BbrPI 1354 CACGTG CAC^GTG
BbsI 2031 GAAGAC GAAGAC(2/6)
BbvCI 1197 CCTCAGC CCTCAGC(-5/-2)
BcgI 1304 CGATGGCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 139 GGATAC GTATCC(6/5)
BfaI 2122 CTAG C^TAG
BglI 307 GCCTTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1494 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1494 GCTCAGC GC^T[ACGT]AGC
BpuAI 2031 GAAGAC GAAGAC(2/6)
BsaBI 2331 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 957 GAATGC GAATGC(1/-1)
BspLU11I 965 ACATGT A^CATGT
CelII 1494 GCTCAGC GC^T[ACGT]AGC
EagI 1171 CGGCCG C^GGCCG
EarI 742 CTCTTC CTCTTC(1/4)
EclXI 1171 CGGCCG C^GGCCG
Eco52I 1171 CGGCCG C^GGCCG
FseI 1172 GGCCGGCC GGCCGG^CC
HincII 2320 GTCAAC GT[CT]^[GA]AC
HindII 2320 GTCAAC GT[CT]^[GA]AC
Ksp632I 742 CTCTTC CTCTTC(1/4)
KspI 734 CCGCGG CCGC^GG
MaeI 2122 CTAG C^TAG
MamI 2331 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1173 GCCGGC GCC^GGC
NgoMIV 1173 GCCGGC G^CCGGC
PinAI 1153 ACCGGT A^CCGGT
PmaCI 1354 CACGTG CAC^GTG
PmlI 1354 CACGTG CAC^GTG
Psp1406I 1830 AACGTT AA^CGTT
SacI 2416 GAGCTC GAGCT^C
SacII 734 CCGCGG CCGC^GG
ScaI 236 AGTACT AGT^ACT
SgrAI 270 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 2604 CCCGGG CCC^GGG
SspI 1638 AATATT AAT^ATT
Tsp509I 1555 AATT ^AATT
XmaCI 2604 CCCGGG C^CCGGG
XmaI 2604 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AocI 1955 CCTCAGG CC^T[ACGT]AGG
2587 CCTCAGG
ApaI 2712 GGGCCC GGGCC^C
3037 GGGCCC
BsaAI 340 TACGTG [CT]AC^GT[GA]
1354 CACGTG
BsaI 407 GAGACC GGTCTC(1/5)
2845 GAGACC
BsiEI 731 CGACCG CG[GA][CT]^CG
1171 CGGCCG
BsrDI 539 GCAATG GCAATG(2/0)
2228 GCAATG
BssHII 1372 GCGCGC G^CGCGC
1510 GCGCGC
Bsu36I 1955 CCTCAGG CC^T[ACGT]AGG
2587 CCTCAGG
Eco81I 1955 CCTCAGG CC^T[ACGT]AGG
2587 CCTCAGG
EcoNI 1293 CCTCAATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1840 CCTGACACAGG
MseI 1801 TTAA T^TAA
3109 TTAA
NcoI 932 CCATGG C^CATGG
2969 CCATGG
PflMI 962 CCAACATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1350 CCAGCACGTGG
SbfI 1212 CCTGCAGG CCTGCA^GG
2838 CCTGCAGG
SmlI 2802 CTTGAG C^T[CT][GA]AG
2890 CTCAAG
Sse8387I 1212 CCTGCAGG CCTGCA^GG
2838 CCTGCAGG
StuI 599 AGGCCT AGG^CCT
1952 AGGCCT
Tru9I 1801 TTAA T^TAA
3109 TTAA
Tsp45I 1934 GTGAC ^GT[GC]AC
1946 GTGAC
Van91I 962 CCAACATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1350 CCAGCACGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AluI Alw44I AlwI AlwNI ApaLI
AspHI AspI AvaI AvaII AviII
BalI BanI BanII BbeI BbvI
BcnI BmyI BpmI BsaHI BsaJI
BsaWI BseAI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspEI BspMI BsrBI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI MaeII MaeIII MboI
MboII MflI MluNI MnlI MroI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV NspI PflFI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SfoI StyI TaqI TfiI TseI
TspRI Tth111I TthHB8I XcmI XhoII