##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
BamHI BbeI BbrPI BbvCI BglI
BglII BlpI Bpu1102I BsaAI BsaBI
BsiWI Bsp1407I BspDI BsrGI BstAPI
BstBI Bsu36I CelII ClaI CpoI
DraI Eam1105I Eco47III Eco81I EcoRV
EcoT22I FseI HindIII HpaI KasI
KpnI MamI MluI NarI NdeI
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI PvuII RsrII SacI
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI XbaI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccIII 702 TCCGGA T^CCGGA
AclI 3222 AACGTT AA^CGTT
AcyI 96 GACGCC G[GA]^CG[CT]C
AflII 668 CTTAAG C^TTAAG
AspI 2057 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2247 TGCGCA TGC^GCA
AvrII 550 CCTAGG C^CTAGG
BanI 1675 GGTGCC G^G[CT][GA]CC
BcgI 2 CGAAGCGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 668 CTTAAG C^TTAAG
BlnI 550 CCTAGG C^CTAGG
BsaHI 96 GACGCC G[GA]^CG[CT]C
BsaI 1568 GAGACC GGTCTC(1/5)
BseAI 702 TCCGGA T^CCGGA
BseRI 1136 GAGGAG GAGGAG(10/8)
BsmBI 454 GAGACG CGTCTC(1/5)
BspEI 702 TCCGGA T^CCGGA
BsrDI 1855 CATTGC GCAATG(2/0)
BssSI 2019 CTCGTG CACGAG(-5/-1)
BstEII 1684 GGTAACC G^GT[ACGT]ACC
BstPI 1684 GGTAACC G^GT[ACGT]ACC
BstXI 2265 CCAAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 1909 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2169 GACCTGGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 245 CGGCCG C^GGCCG
EclXI 245 CGGCCG C^GGCCG
Eco52I 245 CGGCCG C^GGCCG
EcoO65I 1684 GGTAACC G^GT[ACGT]ACC
EcoRI 1374 GAATTC G^AATTC
FspI 2247 TGCGCA TGC^GCA
Hin1I 96 GACGCC G[GA]^CG[CT]C
HincII 1249 GTTGAC GT[CT]^[GA]AC
HindII 1249 GTTGAC GT[CT]^[GA]AC
KspI 225 CCGCGG CCGC^GG
MfeI 960 CAATTG C^AATTG
MroI 702 TCCGGA T^CCGGA
MunI 960 CAATTG C^AATTG
NaeI 243 GCCGGC GCC^GGC
NgoMIV 243 GCCGGC G^CCGGC
NheI 901 GCTAGC G^CTAGC
PaeR7I 1029 CTCGAG C^TCGAG
PflFI 2057 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 162 CCACCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 3222 AACGTT AA^CGTT
PstI 889 CTGCAG CTGCA^G
SacII 225 CCGCGG CCGC^GG
SapI 172 GAAGAGC GCTCTTC(1/4)
Tth111I 2057 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 162 CCACCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1029 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 662 GTATAC GT^[AC][GT]AC
2648 GTATAC
AcsI 1374 GAATTC [GA]^AATT[CT]
1769 AAATTC
AflIII 1282 ACATGT A^C[GA][CT]GT
3201 ACATGT
ApoI 1374 GAATTC [GA]^AATT[CT]
1769 AAATTC
AspHI 1507 GTGCTC G[AT]GC[AT]^C
1757 GAGCAC
AvaI 1029 CTCGAG C^[CT]CG[GA]G
3050 CCCGAG
BanII 180 GAGCCC G[GA]GC[CT]^C
3053 GAGCCC
BbsI 1694 GAAGAC GAAGAC(2/6)
2657 GAAGAC
BciVI 603 GTATCC GTATCC(6/5)
1747 GTATCC
BclI 1068 TGATCA T^GATCA
2182 TGATCA
BcnI 184 CCCGG CC^[GC]GG
248 CCGGG
BpuAI 1694 GAAGAC GAAGAC(2/6)
2657 GAAGAC
BsaWI 702 TCCGGA [AT]^CCGG[AT]
1620 TCCGGT
BsiHKAI 1507 GTGCTC G[AT]GC[AT]^C
1757 GAGCAC
BsmI 1150 GAATGC GAATGC(1/-1)
1865 GAATGC
BsoBI 1029 CTCGAG C^[CT]CG[GA]G
3050 CCCGAG
BspHI 1086 TCATGA T^CATGA
1587 TCATGA
BspLU11I 1282 ACATGT A^CATGT
3201 ACATGT
BspMI 2244 ACCTGC ACCTGC(4/8)
3011 ACCTGC
BsrBI 208 CCGCTC CCGCTC(-3/-3)
1226 GAGCGG
Bst1107I 662 GTATAC GTA^TAC
2648 GTATAC
BstZ17I 662 GTATAC GTA^TAC
2648 GTATAC
FbaI 1068 TGATCA T^GATCA
2182 TGATCA
HaeII 2126 GGCGCT [GA]GCGC^[CT]
2746 GGCGCT
MslI 1475 CACGGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1481 CATGTGGGTG
NciI 184 CCCGG CC^[GC]GG
248 CCGGG
NcoI 264 CCATGG C^CATGG
2421 CCATGG
PpuMI 1894 GGGTCCT [GA]G^G[AT]CC[CT]
2974 GGGACCT
RcaI 1086 TCATGA T^CATGA
1587 TCATGA
Tsp45I 426 GTGAC ^GT[GC]AC
2508 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AvaII BalI BbvI BfaI
BmyI BpmI BsaJI BsgI BsiEI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrFI BsrI BssHII BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
XcmI XhoII