##################################
# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII AccI AccII AclI AcyI
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AspEI AspHI AvaI
AviII AvrII BalI BanII BbeI
BbrPI BbvCI BciVI BglI BlnI
BlpI BmyI Bpu1102I BsaHI BsaI
BsaJI BsgI BsiEI BsiHKAI BsiWI
BsoBI Bsp1286I Bsp1407I BspDI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstNI BstUI
BstXI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoRII
EcoT14I FseI FspI HaeII HhaI
Hin1I HinP1I HpaI KasI KspI
MluI MluNI MscI MspA1I MvaI
MvnI NaeI NarI NcoI NgoMIV
NotI NruI PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI StyI Van91I XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 411 GGTACC G^GTACC
AccIII 257 TCCGGA T^CCGGA
AlwNI 2606 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 276 CCTCAGG CC^T[ACGT]AGG
Asp700I 2537 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 411 GGTACC G^GTACC
AspI 5 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 472 GGTCC G^G[AT]CC
BamHI 2342 GGATCC G^GATCC
BcgI 437 CGATCCTCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 353 TGATCA T^GATCA
BcnI 1020 CCCGG CC^[GC]GG
BsaAI 2764 CACGTA [CT]AC^GT[GA]
BseAI 257 TCCGGA T^CCGGA
BsmFI 107 GGGAC GGGAC(10/14)
BsmI 153 GCATTC GAATGC(1/-1)
BspEI 257 TCCGGA T^CCGGA
BspHI 644 TCATGA T^CATGA
BssKI 1020 CCCGG ^CC[ACGT]GG
BstBI 156 TTCGAA TT^CGAA
BstEII 1083 GGTGACC G^GT[ACGT]ACC
BstPI 1083 GGTGACC G^GT[ACGT]ACC
Bsu36I 276 CCTCAGG CC^T[ACGT]AGG
Eco81I 276 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1083 GGTGACC G^GT[ACGT]ACC
EcoRV 583 GATATC GAT^ATC
FauI 616 CCCGC CCCGC(4/6)
FbaI 353 TGATCA T^GATCA
HincII 1876 GTTGAC GT[CT]^[GA]AC
HindII 1876 GTTGAC GT[CT]^[GA]AC
HindIII 310 AAGCTT A^AGCTT
KpnI 411 GGTACC GGTAC^C
MfeI 1811 CAATTG C^AATTG
MroI 257 TCCGGA T^CCGGA
MunI 1811 CAATTG C^AATTG
MwoI 447 GCTACATTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 1020 CCCGG CC^[GC]GG
NheI 33 GCTAGC G^CTAGC
NspV 156 TTCGAA TT^CGAA
PflFI 5 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 644 TCATGA T^CATGA
ScrFI 1020 CCCGG CC^[ACGT]GG
SfuI 156 TTCGAA TT^CGAA
SwaI 1621 ATTTAAAT ATTT^AAAT
Tsp45I 1084 GTGAC ^GT[GC]AC
Tth111I 5 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1586 TCTAGA T^CTAGA
XmnI 2537 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AciI 617 CCGC CCGC(-3/-1)
782 GCGG
AflII 24 CTTAAG C^TTAAG
1749 CTTAAG
AflIII 1570 ACATGT A^C[GA][CT]GT
2182 ACATGT
BanI 411 GGTACC G^G[CT][GA]CC
792 GGCACC
BbsI 499 GAAGAC GAAGAC(2/6)
2008 GTCTTC
BfrI 24 CTTAAG C^TTAAG
1749 CTTAAG
BglII 741 AGATCT A^GATCT
1757 AGATCT
BpmI 634 CTCCAG CTGGAG(16/14)
2603 CTCCAG
BpuAI 499 GAAGAC GAAGAC(2/6)
2008 GTCTTC
BsaBI 543 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1620 GATTTAAATC
BsaWI 257 TCCGGA [AT]^CCGG[AT]
418 TCCGGT
BsiYI 810 CCAAGAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1021 CCGGAATGAGG
BslI 810 CCAAGAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1021 CCGGAATGAGG
BsmBI 3 GAGACG CGTCTC(1/5)
1309 GAGACG
BspLU11I 1570 ACATGT A^CATGT
2182 ACATGT
Cac8I 15 GCTTGC GC[ACGT]^[ACGT]GC
33 GCTAGC
Cfr13I 472 GGTCC G^G[ACGT]CC
609 GGGCC
EarI 666 GAAGAG CTCTTC(1/4)
1590 GAAGAG
EcoRI 599 GAATTC G^AATTC
1253 GAATTC
EcoT22I 1679 ATGCAT ATGCA^T
2157 ATGCAT
HaeIII 610 GGCC GG^CC
2716 GGCC
HgaI 5 GACGC GACGC(5/10)
109 GACGC
Ksp632I 666 GAAGAG CTCTTC(1/4)
1590 GAAGAG
MamI 543 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1620 GATTTAAATC
NdeI 1857 CATATG CA^TATG
2798 CATATG
NsiI 1679 ATGCAT ATGCA^T
2157 ATGCAT
PleI 2678 GACTC GAGTC(4/5)
1446 GAGTC
Sau96I 472 GGTCC G^G[ACGT]CC
609 GGGCC
ScaI 1145 AGTACT AGT^ACT
2772 AGTACT
SfaNI 1678 GATGC GCATC(5/9)
1681 GCATC
SmlI 24 CTTAAG C^T[CT][GA]AG
1749 CTTAAG
TaqI 157 TCGA T^CGA
707 TCGA
TthHB8I 157 TCGA T^CGA
707 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AseI AsnI BbvI BfaI BseRI
BsmAI BsrI BstF5I BstYI DdeI
DpnII DraI Fnu4HI FokI HapII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI NdeII
NlaIII NlaIV NspI PshBI RsaI
Sau3AI SfcI SspI TfiI Tru9I
TseI Tsp509I TspRI XhoII