##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaLI AscI Asp700I
AspEI AvrII BamHI BbeI BbvCI
BciVI BglII BlnI BsaBI BsmBI
Bsp1407I BsrBI BsrGI BssHII Bst1107I
BstEII BstPI BstZ17I Bsu36I CpoI
DraI DrdI Eam1105I Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FseI HaeII HindIII HpaI KasI
MamI NarI NdeI NheI NotI
NruI NsiI PacI PaeR7I PinAI
PmeI PvuI RsrII SacI SbfI
SexAI SfoI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SwaI XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
Acc65I 700 GGTACC G^GTACC
AccI 627 GTCGAC GT^[AC][GT]AC
AccIII 445 TCCGGA T^CCGGA
AclI 2211 AACGTT AA^CGTT
AcsI 2163 AAATTT [GA]^AATT[CT]
AcyI 630 GACGCC G[GA]^CG[CT]C
AflII 2446 CTTAAG C^TTAAG
ApaI 55 GGGCCC GGGCC^C
ApoI 2163 AAATTT [GA]^AATT[CT]
AseI 2487 ATTAAT AT^TAAT
AsnI 2487 ATTAAT AT^TAAT
Asp718I 700 GGTACC G^GTACC
AspI 2389 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 1913 GAAGAC GAAGAC(2/6)
BcgI 106 GCAAGGCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1035 CTAG C^TAG
BfrI 2446 CTTAAG C^TTAAG
BlpI 1324 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1324 GCTCAGC GC^T[ACGT]AGC
BpuAI 1913 GAAGAC GAAGAC(2/6)
BsaHI 630 GACGCC G[GA]^CG[CT]C
BsaI 1776 GAGACC GGTCTC(1/5)
BsaWI 445 TCCGGA [AT]^CCGG[AT]
BseAI 445 TCCGGA T^CCGGA
BsiWI 1319 CGTACG C^GTACG
BsmI 981 GCATTC GAATGC(1/-1)
BspDI 495 ATCGAT AT^CGAT
BspEI 445 TCCGGA T^CCGGA
BspLU11I 2366 ACATGT A^CATGT
BsrDI 1343 CATTGC GCAATG(2/0)
BssSI 1608 CACGAG CACGAG(-5/-1)
BstAPI 2463 GCACTTTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1851 TTCGAA TT^CGAA
CelII 1324 GCTCAGC GC^T[ACGT]AGC
ClaI 495 ATCGAT AT^CGAT
DraIII 1086 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
Hin1I 630 GACGCC G[GA]^CG[CT]C
HincII 627 GTCGAC GT[CT]^[GA]AC
HindII 627 GTCGAC GT[CT]^[GA]AC
KpnI 700 GGTACC GGTAC^C
KspI 1297 CCGCGG CCGC^GG
MaeI 1035 CTAG C^TAG
MluI 1475 ACGCGT A^CGCGT
MroI 445 TCCGGA T^CCGGA
NaeI 102 GCCGGC GCC^GGC
NgoMIV 102 GCCGGC G^CCGGC
NspV 1851 TTCGAA TT^CGAA
PflFI 2389 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 227 CCAGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1424 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2487 ATTAAT AT^TAAT
Psp1406I 2211 AACGTT AA^CGTT
SacII 1297 CCGCGG CCGC^GG
SalI 627 GTCGAC G^TCGAC
SapI 1854 GAAGAGC GCTCTTC(1/4)
ScaI 1492 AGTACT AGT^ACT
SfiI 101 GGCCGGCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1851 TTCGAA TT^CGAA
SspI 834 AATATT AAT^ATT
Tth111I 2389 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 227 CCAGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AviII 175 TGCGCA TGC^GCA
1519 TGCGCA
BbrPI 934 CACGTG CAC^GTG
954 CACGTG
BclI 1244 TGATCA T^GATCA
1546 TGATCA
BglI 102 GCCGGCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1447 GCCAAGGAGGC
BsmAI 1776 GAGAC GTCTC(1/5)
279 GTCTC
BspHI 637 TCATGA T^CATGA
2305 TCATGA
BspMI 373 ACCTGC ACCTGC(4/8)
1719 ACCTGC
BsrFI 102 GCCGGC [GA]^CCGG[CT]
1443 ACCGGC
Cfr10I 102 GCCGGC [GA]^CCGG[CT]
1443 ACCGGC
EagI 1315 CGGCCG C^GGCCG
1825 CGGCCG
EclXI 1315 CGGCCG C^GGCCG
1825 CGGCCG
Eco52I 1315 CGGCCG C^GGCCG
1825 CGGCCG
FbaI 1244 TGATCA T^GATCA
1546 TGATCA
FspI 175 TGCGCA TGC^GCA
1519 TGCGCA
MfeI 859 CAATTG C^AATTG
1262 CAATTG
MseI 2447 TTAA T^TAA
2488 TTAA
MunI 859 CAATTG C^AATTG
1262 CAATTG
PmaCI 934 CACGTG CAC^GTG
954 CACGTG
PmlI 934 CACGTG CAC^GTG
954 CACGTG
PpuMI 663 AGGACCT [GA]G^G[AT]CC[CT]
969 GGGACCC
RcaI 637 TCATGA T^CATGA
2305 TCATGA
Tru9I 2447 TTAA T^TAA
2488 TTAA
XcmI 765 CCAGAGGAAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2182 CCATTTGTGTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AspHI AvaI AvaII BalI
BanI BanII BbvI BcnI BmyI
BpmI BsaAI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StuI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmaCI XmaI