##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI AhdI Alw44I ApaLI AscI
Asp700I Asp718I AspEI AviII AvrII
BbeI BbrPI BcgI BciVI BclI
BlnI BlpI Bpu1102I BsaAI BsaWI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspLU11I BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI DraIII EagI
Eam1105I EclXI Eco52I EcoO65I EcoT22I
FbaI FseI FspI HindIII HpaI
KasI KpnI KspI MfeI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI SexAI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI TaqI TthHB8I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 2626 GTCTAC GT^[AC][GT]AC
AccII 1506 CGCG CG^CG
AcyI 1036 GACGCC G[GA]^CG[CT]C
AflII 1595 CTTAAG C^TTAAG
Aor51HI 1623 AGCGCT AGC^GCT
AseI 899 ATTAAT AT^TAAT
AsnI 899 ATTAAT AT^TAAT
AspHI 1025 GTGCTC G[AT]GC[AT]^C
AspI 1914 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1428 GGATCC G^GATCC
BbsI 206 GAAGAC GAAGAC(2/6)
BfrI 1595 CTTAAG C^TTAAG
BpuAI 206 GAAGAC GAAGAC(2/6)
BsaBI 1664 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1036 GACGCC G[GA]^CG[CT]C
BsiHKAI 1025 GTGCTC G[AT]GC[AT]^C
BspHI 1857 TCATGA T^CATGA
BsrDI 2095 CATTGC GCAATG(2/0)
BsrFI 2396 GCCGGT [GA]^CCGG[CT]
BssSI 2121 CTCGTG CACGAG(-5/-1)
BstAPI 1462 GCAGAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1506 CGCG CG^CG
Cfr10I 2396 GCCGGT [GA]^CCGG[CT]
DraI 377 TTTAAA TTT^AAA
DrdI 964 GACCTGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 144 CCATGG C^C[GA][CT]GG
EarI 917 GAAGAG CTCTTC(1/4)
Eco47III 1623 AGCGCT AGC^GCT
EcoRI 2331 GAATTC G^AATTC
EcoRV 1643 GATATC GAT^ATC
Hin1I 1036 GACGCC G[GA]^CG[CT]C
HincII 2581 GTTGAC GT[CT]^[GA]AC
HindII 2581 GTTGAC GT[CT]^[GA]AC
Ksp632I 917 GAAGAG CTCTTC(1/4)
MamI 1664 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 1506 CGCG CG^CG
NcoI 144 CCATGG C^CATGG
PflFI 1914 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 899 ATTAAT AT^TAAT
RcaI 1857 TCATGA T^CATGA
ScaI 1584 AGTACT AGT^ACT
SmaI 2202 CCCGGG CCC^GGG
SmlI 1595 CTTAAG C^T[CT][GA]AG
Tth111I 1914 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2563 TCTAGA T^CTAGA
XmaCI 2202 CCCGGG C^CCGGG
XmaI 2202 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AocI 99 CCTGAGG CC^T[ACGT]AGG
1059 CCTCAGG
BanI 1612 GGTGCC G^G[CT][GA]CC
2453 GGTGCC
BbvCI 191 GCTGAGG CCTCAGC(-5/-2)
2189 GCTGAGG
BglII 249 AGATCT A^GATCT
1948 AGATCT
BseRI 176 CTCCTC GAGGAG(10/8)
1829 CTCCTC
BspMI 1467 ACCTGC ACCTGC(4/8)
1485 ACCTGC
BsrBI 120 CCGCTC CCGCTC(-3/-3)
2371 CCGCTC
Bsu36I 99 CCTGAGG CC^T[ACGT]AGG
1059 CCTCAGG
Eco81I 99 CCTGAGG CC^T[ACGT]AGG
1059 CCTCAGG
HaeII 212 AGCGCC [GA]GCGC^[CT]
1623 AGCGCT
HgaI 1036 GACGC GACGC(5/10)
1918 GCGTC
MaeII 1102 ACGT A^CGT
1687 ACGT
MaeIII 989 GTGAC ^GT[ACGT]AC
2124 GTGAC
NspI 1691 GCATGC [GA]CATG^[CT]
2595 GCATGC
PflMI 1221 CCATTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1401 CCACTGACTGG
SfiI 577 GGCCTTCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
751 GGCCAAAATGGCC
SphI 1691 GCATGC GCATG^C
2595 GCATGC
Tsp45I 989 GTGAC ^GT[GC]AC
2124 GTGAC
Van91I 1221 CCATTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1401 CCACTGACTGG
XcmI 597 CCACTACTAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2336 CCATTTACCTTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApaI ApoI AvaI AvaII
BalI BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaI BsaJI
BsgI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI StyI
TfiI Tru9I TseI Tsp509I TspRI
XhoII