##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AccI AccIII AclI AflIII AgeI
AhdI Alw44I AlwNI ApaLI AscI
AseI AsnI AspEI AviII AvrII
BalI BbeI BbrPI BbvCI BcgI
BciVI BglI BlnI BlpI Bpu1102I
BsaAI BsaWI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BspLU11I BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstZ17I CelII Cfr10I ClaI CpoI
DraIII EaeI EagI Eam1105I EclXI
Eco52I EcoNI FseI FspI HgaI
HindIII HpaI KasI KspI MluI
MluNI MroI MscI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI SspBI StuI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 1885 GGTACC G^GTACC
AccII 3047 CGCG CG^CG
AflII 1308 CTTAAG C^TTAAG
AocI 2103 CCTAAGG CC^T[ACGT]AGG
Aor51HI 1828 AGCGCT AGC^GCT
ApaI 1870 GGGCCC GGGCC^C
Asp700I 315 GAACGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1885 GGTACC G^GTACC
AspHI 2530 GTGCTC G[AT]GC[AT]^C
AvaI 3243 CCCGGG C^[CT]CG[GA]G
BclI 2824 TGATCA T^GATCA
BfrI 1308 CTTAAG C^TTAAG
BglII 476 AGATCT A^GATCT
BsaBI 2822 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 1548 GAGGAG GAGGAG(10/8)
BsiHKAI 2530 GTGCTC G[AT]GC[AT]^C
BsoBI 3243 CCCGGG C^[CT]CG[GA]G
BspMI 3188 GCAGGT ACCTGC(4/8)
BsrBI 2387 CCGCTC CCGCTC(-3/-3)
BstEII 1226 GGTCACC G^GT[ACGT]ACC
BstPI 1226 GGTCACC G^GT[ACGT]ACC
BstUI 3047 CGCG CG^CG
Bsu36I 2103 CCTAAGG CC^T[ACGT]AGG
DraI 2507 TTTAAA TTT^AAA
DrdI 372 GACCTCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1141 CCATGG C^C[GA][CT]GG
Eco47III 1828 AGCGCT AGC^GCT
Eco81I 2103 CCTAAGG CC^T[ACGT]AGG
EcoO65I 1226 GGTCACC G^GT[ACGT]ACC
FbaI 2824 TGATCA T^GATCA
HaeII 1828 AGCGCT [GA]GCGC^[CT]
HincII 2621 GTCAAC GT[CT]^[GA]AC
HindII 2621 GTCAAC GT[CT]^[GA]AC
KpnI 1885 GGTACC GGTAC^C
MamI 2822 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 859 CAATTG C^AATTG
MslI 2816 CATAAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 859 CAATTG C^AATTG
MvnI 3047 CGCG CG^CG
NcoI 1141 CCATGG C^CATGG
NdeI 2809 CATATG CA^TATG
PvuII 2073 CAGCTG CAG^CTG
SbfI 1874 CCTGCAGG CCTGCA^GG
SexAI 1273 ACCTGGT A^CC[AT]GGT
SmaI 3243 CCCGGG CCC^GGG
Sse8387I 1874 CCTGCAGG CCTGCA^GG
XbaI 121 TCTAGA T^CTAGA
XcmI 950 CCACTCTCTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 3243 CCCGGG C^CCGGG
XmaI 3243 CCCGGG C^CCGGG
XmnI 315 GAACGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AatII 1175 GACGTC GACGT^C
2728 GACGTC
AcyI 1175 GACGTC G[GA]^CG[CT]C
2728 GACGTC
AspI 3143 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3310 GACTCTGTC
AvaII 2057 GGACC G^G[AT]CC
2776 GGACC
BamHI 281 GGATCC G^GATCC
1685 GGATCC
BanI 1885 GGTACC G^G[CT][GA]CC
3191 GGTGCC
BbsI 1854 GAAGAC GAAGAC(2/6)
117 GTCTTC
BpuAI 1854 GAAGAC GAAGAC(2/6)
117 GTCTTC
BsaHI 1175 GACGTC G[GA]^CG[CT]C
2728 GACGTC
BsaI 3118 GAGACC GGTCTC(1/5)
3141 GAGACC
BsgI 72 GTGCAG GTGCAG(16/14)
2149 GTGCAG
BsmBI 3148 CGTCTC CGTCTC(1/5)
1173 GAGACG
BsmI 491 GAATGC GAATGC(1/-1)
3284 GCATTC
BspHI 569 TCATGA T^CATGA
2126 TCATGA
BsrDI 153 GCAATG GCAATG(2/0)
442 GCAATG
BssSI 1916 CACGAG CACGAG(-5/-1)
3101 CACGAG
BstXI 1259 CCAGTGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3070 CCAGCACTTTGG
EcoRI 712 GAATTC G^AATTC
1427 GAATTC
EcoRV 1080 GATATC GAT^ATC
2286 GATATC
EcoT22I 233 ATGCAT ATGCA^T
997 ATGCAT
FauI 1435 CCCGC CCCGC(4/6)
2217 CCCGC
HapII 3176 CCGG C^CGG
3244 CCGG
Hin1I 1175 GACGTC G[GA]^CG[CT]C
2728 GACGTC
HpaII 3176 CCGG C^CGG
3244 CCGG
MaeII 1176 ACGT A^CGT
2729 ACGT
MspA1I 1453 CAGCGG C[AC]G^C[GT]G
2073 CAGCTG
MspI 3176 CCGG C^CGG
3244 CCGG
NsiI 233 ATGCAT ATGCA^T
997 ATGCAT
NspI 841 ACATGC [GA]CATG^[CT]
1705 ACATGC
PflFI 3143 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3310 GACTCTGTC
PstI 1875 CTGCAG CTGCA^G
3220 CTGCAG
RcaI 569 TCATGA T^CATGA
2126 TCATGA
ScaI 1072 AGTACT AGT^ACT
1576 AGTACT
SspI 35 AATATT AAT^ATT
2277 AATATT
TaqI 427 TCGA T^CGA
2227 TCGA
Tth111I 3143 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3310 GACTCTGTC
TthHB8I 427 TCGA T^CGA
2227 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HhaI HinP1I HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XhoII