##################################
# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI Asp700I Asp718I
AspEI AspI AviII AvrII BanI
BbeI BbrPI BcgI BclI BfrI
BglI BlnI BlpI Bpu1102I BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BsrBI BsrFI BsrGI BssHII Bst1107I
BstEII BstPI BstUI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
Hin1I HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NdeI NgoMIV
NotI NruI NsiI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Tth111I XbaI
XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AseI 2145 ATTAAT AT^TAAT
AsnI 2145 ATTAAT AT^TAAT
AvaI 3 CCCGGG C^[CT]CG[GA]G
BalI 1027 TGGCCA TGG^CCA
BamHI 1371 GGATCC G^GATCC
BbvCI 2512 CCTCAGC CCTCAGC(-5/-2)
BsaAI 312 CACGTA [CT]AC^GT[GA]
BsaI 2825 GAGACC GGTCTC(1/5)
BsoBI 3 CCCGGG C^[CT]CG[GA]G
BspLU11I 1968 ACATGT A^CATGT
BspMI 402 ACCTGC ACCTGC(4/8)
BsrDI 2036 GCAATG GCAATG(2/0)
BstAPI 712 GCAGATTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 262 TTCGAA TT^CGAA
CfoI 201 GCGC GCG^C
DraII 3039 AGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 1739 CCATGG C^C[GA][CT]GG
EaeI 1027 TGGCCA [CT]^GGCC[GA]
EcoO109I 3039 AGGTCCT [GA]G^G[ACGT]CC[CT]
HgaI 653 GACGC GACGC(5/10)
HhaI 201 GCGC GCG^C
HinP1I 201 GCGC G^CGC
HincII 2719 GTCAAC GT[CT]^[GA]AC
HindII 2719 GTCAAC GT[CT]^[GA]AC
HindIII 258 AAGCTT A^AGCTT
MluNI 1027 TGGCCA TGG^CCA
MscI 1027 TGGCCA TGG^CCA
NcoI 1739 CCATGG C^CATGG
NheI 2791 GCTAGC G^CTAGC
NspV 262 TTCGAA TT^CGAA
PpuMI 3039 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 2145 ATTAAT AT^TAAT
SacI 39 GAGCTC GAGCT^C
SfuI 262 TTCGAA TT^CGAA
SmaI 3 CCCGGG CCC^GGG
SspI 2879 AATATT AAT^ATT
XmaCI 3 CCCGGG C^CCGGG
XmaI 3 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 888 GTCTAC GT^[AC][GT]AC
2500 GTCTAC
AflIII 1968 ACATGT A^C[GA][CT]GT
2496 ACACGT
AlwNI 1282 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1399 CAGGATCTG
AocI 1681 CCTGAGG CC^T[ACGT]AGG
3064 CCTGAGG
BanII 39 GAGCTC G[GA]GC[CT]^C
1749 GGGCTC
BbsI 573 GAAGAC GAAGAC(2/6)
1713 GTCTTC
BciVI 485 GTATCC GTATCC(6/5)
832 GTATCC
BglII 188 AGATCT A^GATCT
920 AGATCT
BpuAI 573 GAAGAC GAAGAC(2/6)
1713 GTCTTC
BseRI 2312 GAGGAG GAGGAG(10/8)
3035 GAGGAG
BsmFI 2317 GGGAC GGGAC(10/14)
2166 GTCCC
BspHI 2726 TCATGA T^CATGA
2993 TCATGA
BssSI 2333 CACGAG CACGAG(-5/-1)
2132 CTCGTG
Bsu36I 1681 CCTGAGG CC^T[ACGT]AGG
3064 CCTGAGG
Eco81I 1681 CCTGAGG CC^T[ACGT]AGG
3064 CCTGAGG
EcoNI 1362 CCTTCCGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3060 CCTTCCTGAGG
PflMI 1115 CCAAGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2653 CCAGTGAATGG
RcaI 2726 TCATGA T^CATGA
2993 TCATGA
Van91I 1115 CCAAGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2653 CCAGTGAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AclI AcsI AfaI AluI
AlwI ApoI AspHI AvaII BbvI
BcnI BfaI BmyI BpmI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI Psp1406I PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII