##################################
# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AgeI AhdI AocI ApaI
AscI Asp700I Asp718I AspEI AspI
AvaI AvrII BalI BanI BbeI
BbrPI BciVI BglII BlnI BpmI
BsaHI BsaI BseAI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoO65I FseI Hin1I HindIII HpaI
KasI KpnI KspI MfeI MluI
MluNI MroI MscI MunI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
Tth111I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccII 137 CGCG CG^CG
AflII 834 CTTAAG C^TTAAG
AflIII 587 ACGTGT A^C[GA][CT]GT
Aor51HI 2220 AGCGCT AGC^GCT
AviII 2731 TGCGCA TGC^GCA
BamHI 1891 GGATCC G^GATCC
BanII 333 GAGCCC G[GA]GC[CT]^C
BbvCI 157 GCTGAGG CCTCAGC(-5/-2)
BcnI 83 CCCGG CC^[GC]GG
BfrI 834 CTTAAG C^TTAAG
BglI 3 GCCCGTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2754 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 2754 GCTTAGC GC^T[ACGT]AGC
BsaWI 769 TCCGGT [AT]^CCGG[AT]
BspHI 917 TCATGA T^CATGA
BsrBI 1726 GAGCGG CCGCTC(-3/-3)
BsrDI 2566 GCAATG GCAATG(2/0)
BstUI 137 CGCG CG^CG
CelII 2754 GCTTAGC GC^T[ACGT]AGC
DraII 2194 AGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 146 CCGTGG C^C[GA][CT]GG
Eco47III 2220 AGCGCT AGC^GCT
EcoO109I 2194 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 409 GAATTC G^AATTC
FspI 2731 TGCGCA TGC^GCA
HincII 804 GTCAAC GT[CT]^[GA]AC
HindII 804 GTCAAC GT[CT]^[GA]AC
MvnI 137 CGCG CG^CG
NciI 83 CCCGG CC^[GC]GG
NdeI 721 CATATG CA^TATG
PvuII 2090 CAGCTG CAG^CTG
RcaI 917 TCATGA T^CATGA
SapI 495 GAAGAGC GCTCTTC(1/4)
SspI 667 AATATT AAT^ATT
SwaI 2929 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Alw44I 1240 GTGCAC G^TGCAC
2035 GTGCAC
ApaLI 1240 GTGCAC G^TGCAC
2035 GTGCAC
AspHI 1240 GTGCAC G[AT]GC[AT]^C
2035 GTGCAC
BcgI 179 GCAGGCGGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
404 GCATCGAATTCG
BclI 2535 TGATCA T^GATCA
3298 TGATCA
BsaAI 586 TACGTG [CT]AC^GT[GA]
2838 TACGTG
BsaBI 2160 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3299 GATCACCATC
BsgI 2274 CTGCAC GTGCAG(16/14)
3372 GTGCAG
BsiHKAI 1240 GTGCAC G[AT]GC[AT]^C
2035 GTGCAC
BstYI 1891 GGATCC [GA]^GATC[CT]
2266 AGATCC
EaeI 937 CGGCCA [CT]^GGCC[GA]
3044 TGGCCG
EcoNI 1251 CCTTTTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2663 CCTTTTTAAGG
EcoRV 351 GATATC GAT^ATC
1157 GATATC
EcoT14I 337 CCTTGG C^C[AT][AT]GG
1773 CCTTGG
EcoT22I 2798 ATGCAT ATGCA^T
2854 ATGCAT
FbaI 2535 TGATCA T^GATCA
3298 TGATCA
HapII 84 CCGG C^CGG
770 CCGG
HpaII 84 CCGG C^CGG
770 CCGG
MamI 2160 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3299 GATCACCATC
MflI 1891 GGATCC [GA]^GATC[CT]
2266 AGATCC
MspA1I 2090 CAGCTG C[AC]G^C[GT]G
2271 CCGCTG
MspI 84 CCGG C^CGG
770 CCGG
NsiI 2798 ATGCAT ATGCA^T
2854 ATGCAT
NspI 441 GCATGT [GA]CATG^[CT]
907 GCATGT
SmlI 834 CTTAAG C^T[CT][GA]AG
2789 CTCAAG
StyI 337 CCTTGG C^C[AT][AT]GG
1773 CCTTGG
XhoII 1891 GGATCC [GA]^GATC[CT]
2266 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI AvaII
BbsI BbvI BfaI BmyI BpuAI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraI EarI EcoRII FauI
Fnu4HI FokI HaeII HaeIII HgaI
HhaI HinP1I HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV PflMI
PleI PshBI PstI RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I