##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AflIII AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BamHI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BlnI BpmI BsaAI
BseAI BseRI BsiEI BsmBI BspDI
BspEI BspLU11I BspMI BssHII Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV FbaI
FseI FspI HgaI HindIII MluI
MroI NaeI NdeI NgoMIV NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SalI SbfI ScaI
SfiI SfuI SmaI SnaBI SpeI
SphI Sse8387I StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 119 GGTACC G^GTACC
AccII 18 CGCG CG^CG
AcyI 79 GGCGCC G[GA]^CG[CT]C
AgeI 358 ACCGGT A^CCGGT
ApaI 236 GGGCCC GGGCC^C
Asp718I 119 GGTACC G^GTACC
AvaI 212 CCCGAG C^[CT]CG[GA]G
AvaII 1336 GGACC G^G[AT]CC
BbeI 79 GGCGCC GGCGC^C
BbsI 670 GAAGAC GAAGAC(2/6)
BpuAI 670 GAAGAC GAAGAC(2/6)
BsaBI 1411 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 79 GGCGCC G[GA]^CG[CT]C
BsaWI 358 ACCGGT [AT]^CCGG[AT]
BsiWI 159 CGTACG C^GTACG
BsmI 2455 GAATGC GAATGC(1/-1)
BsoBI 212 CCCGAG C^[CT]CG[GA]G
Bsp1407I 2376 TGTACA T^GTACA
BspHI 2123 TCATGA T^CATGA
BsrBI 105 GAGCGG CCGCTC(-3/-3)
BsrGI 2376 TGTACA T^GTACA
BssSI 148 CACGAG CACGAG(-5/-1)
BstAPI 724 GCAAATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 1809 GGATG GGATG(2/0)
BstUI 18 CGCG CG^CG
DrdI 1151 GACAGACTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1576 CCTTTTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 1809 GGATG GGATG(9/13)
Hin1I 79 GGCGCC G[GA]^CG[CT]C
KasI 79 GGCGCC G^GCGCC
KpnI 119 GGTACC GGTAC^C
KspI 17 CCGCGG CCGC^GG
MaeII 158 ACGT A^CGT
MamI 1411 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 18 CGCG CG^CG
NarI 79 GGCGCC GG^CGCC
NcoI 1628 CCATGG C^CATGG
PflMI 147 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 358 ACCGGT A^CCGGT
PstI 554 CTGCAG CTGCA^G
RcaI 2123 TCATGA T^CATGA
SacII 17 CCGCGG CCGC^GG
SfoI 79 GGCGCC GGC^GCC
SgrAI 357 CACCGGTG C[GA]^CCGG[CT]G
SspBI 2376 TGTACA T^GTACA
Van91I 147 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 83 CCAGACAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AspHI 887 GTGCTC G[AT]GC[AT]^C
934 GTGCTC
BglII 572 AGATCT A^GATCT
856 AGATCT
BlpI 126 GCTGAGC GC^T[ACGT]AGC
1099 GCTGAGC
Bpu1102I 126 GCTGAGC GC^T[ACGT]AGC
1099 GCTGAGC
BsaI 215 GAGACC GGTCTC(1/5)
54 GGTCTC
BsgI 1781 CTGCAC GTGCAG(16/14)
1934 CTGCAC
BsiHKAI 887 GTGCTC G[AT]GC[AT]^C
934 GTGCTC
BsrDI 2196 CATTGC GCAATG(2/0)
1991 GCAATG
BsrFI 122 ACCGGC [GA]^CCGG[CT]
358 ACCGGT
CelII 126 GCTGAGC GC^T[ACGT]AGC
1099 GCTGAGC
Cfr10I 122 ACCGGC [GA]^CCGG[CT]
358 ACCGGT
DraII 164 GGGGCCT [GA]G^G[ACGT]CC[CT]
236 GGGCCCC
DsaI 17 CCGCGG C^C[GA][CT]GG
1628 CCATGG
EcoO109I 164 GGGGCCT [GA]G^G[ACGT]CC[CT]
236 GGGCCCC
EcoT14I 1375 CCTTGG C^C[AT][AT]GG
1628 CCATGG
EcoT22I 312 ATGCAT ATGCA^T
381 ATGCAT
FauI 13 CCCGC CCCGC(4/6)
227 CCCGC
HaeII 26 GGCGCT [GA]GCGC^[CT]
79 GGCGCC
HpaI 1380 GTTAAC GTT^AAC
1800 GTTAAC
HphI 946 GGTGA GGTGA(8/7)
1231 GGTGA
MfeI 1316 CAATTG C^AATTG
2091 CAATTG
MslI 1123 CATCTTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1966 CACCCCAGTG
MunI 1316 CAATTG C^AATTG
2091 CAATTG
NsiI 312 ATGCAT ATGCA^T
381 ATGCAT
PleI 1155 GACTC GAGTC(4/5)
36 GAGTC
SapI 5 GCTCTTC GCTCTTC(1/4)
1538 GCTCTTC
SmlI 324 CTCAAG C^T[CT][GA]AG
1825 CTTGAG
SspI 1693 AATATT AAT^ATT
1749 AATATT
StyI 1375 CCTTGG C^C[AT][AT]GG
1628 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI BalI BanI BanII
BbvI BcnI BfaI BmyI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EaeI
EarI EcoRII Fnu4HI HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PvuII RsaI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII