##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
AvrII BamHI BbeI BcgI BciVI
BfaI BfrI BglII BlnI BsaI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstXI BstZ17I ClaI CpoI DrdI
Eco47III EcoO65I EcoRI EcoRV FseI
HincII HindII HpaI KasI KpnI
KspI MaeI MfeI MluI MroI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NruI NspI NspV
PacI PflFI PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 2168 GACGTC GACGT^C
AflIII 1437 ACGTGT A^C[GA][CT]GT
AhdI 548 GACTTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 548 GACTTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1268 GAGCTC G[AT]GC[AT]^C
AviII 1935 TGCGCA TGC^GCA
BbrPI 1436 CACGTG CAC^GTG
BbvCI 1877 CCTCAGC CCTCAGC(-5/-2)
BclI 1530 TGATCA T^GATCA
BglI 128 GCCAGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1453 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1453 GCTCAGC GC^T[ACGT]AGC
BsaBI 1531 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 552 TCCGGT [AT]^CCGG[AT]
BsiHKAI 1268 GAGCTC G[AT]GC[AT]^C
BsrDI 1938 GCAATG GCAATG(2/0)
BsrFI 1418 GCCGGT [GA]^CCGG[CT]
BssSI 1687 CTCGTG CACGAG(-5/-1)
CelII 1453 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1418 GCCGGT [GA]^CCGG[CT]
DraI 2252 TTTAAA TTT^AAA
Eam1105I 548 GACTTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 587 GAAGAG CTCTTC(1/4)
EcoT22I 2285 ATGCAT ATGCA^T
FbaI 1530 TGATCA T^GATCA
FspI 1935 TGCGCA TGC^GCA
HindIII 1259 AAGCTT A^AGCTT
Ksp632I 587 GAAGAG CTCTTC(1/4)
MamI 1531 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 1273 CATCCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NotI 313 GCGGCCGC GC^GGCCGC
NsiI 2285 ATGCAT ATGCA^T
PaeR7I 1755 CTCGAG C^TCGAG
PflMI 502 CCAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1436 CACGTG CAC^GTG
PmlI 1436 CACGTG CAC^GTG
PshAI 433 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1268 GAGCTC GAGCT^C
ScaI 834 AGTACT AGT^ACT
SmaI 912 CCCGGG CCC^GGG
TfiI 1898 GATTC G^A[AT]TC
Van91I 502 CCAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1755 CTCGAG C^TCGAG
XmaCI 912 CCCGGG C^CCGGG
XmaI 912 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 2255 AAATTT [GA]^AATT[CT]
2600 AAATTC
AcyI 1385 GACGCC G[GA]^CG[CT]C
2168 GACGTC
AocI 470 CCTGAGG CC^T[ACGT]AGG
1632 CCTGAGG
ApoI 2255 AAATTT [GA]^AATT[CT]
2600 AAATTC
BbsI 382 GAAGAC GAAGAC(2/6)
1111 GAAGAC
BpuAI 382 GAAGAC GAAGAC(2/6)
1111 GAAGAC
BsaAI 1436 CACGTG [CT]AC^GT[GA]
1902 CACGTA
BsaHI 1385 GACGCC G[GA]^CG[CT]C
2168 GACGTC
BspMI 981 ACCTGC ACCTGC(4/8)
1961 GCAGGT
BstAPI 1961 GCAGGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2070 GCACCAGATGC
BstYI 729 AGATCC [GA]^GATC[CT]
1287 AGATCC
Bsu36I 470 CCTGAGG CC^T[ACGT]AGG
1632 CCTGAGG
DraIII 564 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
620 CACCGCGTG
DsaI 2397 CCACGG C^C[GA][CT]GG
2529 CCACGG
EagI 314 CGGCCG C^GGCCG
1171 CGGCCG
EclXI 314 CGGCCG C^GGCCG
1171 CGGCCG
Eco52I 314 CGGCCG C^GGCCG
1171 CGGCCG
Eco81I 470 CCTGAGG CC^T[ACGT]AGG
1632 CCTGAGG
EcoT14I 756 CCAAGG C^C[AT][AT]GG
1389 CCAAGG
HaeII 145 AGCGCC [GA]GCGC^[CT]
953 GGCGCT
Hin1I 1385 GACGCC G[GA]^CG[CT]C
2168 GACGTC
MflI 729 AGATCC [GA]^GATC[CT]
1287 AGATCC
PleI 466 GACTC GAGTC(4/5)
2458 GACTC
SexAI 1496 ACCAGGT A^CC[AT]GGT
2062 ACCTGGT
StyI 756 CCAAGG C^C[AT][AT]GG
1389 CCAAGG
XhoII 729 AGATCC [GA]^GATC[CT]
1287 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AvaI AvaII BalI
BanI BanII BbvI BcnI BmyI
BpmI BsaJI BseRI BsgI BsiEI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrBI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EcoNI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI