##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
AocI Aor51HI AscI Asp718I AspI
AviII BalI BamHI BbrPI BbsI
BcgI BfrI BglI BlpI Bpu1102I
BpuAI BsaBI BsaI BseAI BsiWI
BspEI BspHI BspMI BsrFI BssHII
BssSI BstBI BstEII BstPI Bsu36I
CelII Cfr10I DraIII Eco47III Eco81I
EcoO65I EcoRI EcoRV FseI FspI
HindIII HpaI KpnI MamI MluI
MluNI MroI MscI NaeI NcoI
NdeI NgoMIV NheI NruI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PpuMI PvuI RcaI SalI
SapI SbfI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
StuI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AclI 431 AACGTT AA^CGTT
AhdI 526 GACAATATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1781 GTGCAC G^TGCAC
ApaLI 1781 GTGCAC G^TGCAC
AseI 2421 ATTAAT AT^TAAT
AsnI 2421 ATTAAT AT^TAAT
Asp700I 2134 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 526 GACAATATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 124 CCTAGG C^CTAGG
BbeI 172 GGCGCC GGCGC^C
BciVI 1576 GGATAC GTATCC(6/5)
BclI 3357 TGATCA T^GATCA
BglII 1805 AGATCT A^GATCT
BlnI 124 CCTAGG C^CTAGG
BsaAI 1579 TACGTG [CT]AC^GT[GA]
BsaWI 2052 ACCGGA [AT]^CCGG[AT]
BsiEI 135 CGGCCG CG[GA][CT]^CG
BsmBI 811 CGTCTC CGTCTC(1/5)
BsmI 1447 GCATTC GAATGC(1/-1)
BspDI 2513 ATCGAT AT^CGAT
BstXI 1199 CCAGTATACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1805 AGATCT [GA]^GATC[CT]
ClaI 2513 ATCGAT AT^CGAT
CpoI 223 CGGTCCG CG^G[AT]CCG
DrdI 1732 GACCACGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 135 CGGCCG C^GGCCG
Eam1105I 526 GACAATATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 2363 CTCTTC CTCTTC(1/4)
EclXI 135 CGGCCG C^GGCCG
Eco52I 135 CGGCCG C^GGCCG
EcoT22I 2016 ATGCAT ATGCA^T
FbaI 3357 TGATCA T^GATCA
HincII 1616 GTCAAC GT[CT]^[GA]AC
HindII 1616 GTCAAC GT[CT]^[GA]AC
KasI 172 GGCGCC G^GCGCC
Ksp632I 2363 CTCTTC CTCTTC(1/4)
MflI 1805 AGATCT [GA]^GATC[CT]
NarI 172 GGCGCC GG^CGCC
NotI 134 GCGGCCGC GC^GGCCGC
NsiI 2016 ATGCAT ATGCA^T
PaeR7I 216 CTCGAG C^TCGAG
PflMI 142 CCAGGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 2421 ATTAAT AT^TAAT
Psp1406I 431 AACGTT AA^CGTT
RsrII 223 CGGTCCG CG^G[AT]CCG
ScaI 2136 AGTACT AGT^ACT
SfoI 172 GGCGCC GGC^GCC
SmaI 208 CCCGGG CCC^GGG
SmlI 216 CTCGAG C^T[CT][GA]AG
Van91I 142 CCAGGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 216 CTCGAG C^TCGAG
XhoII 1805 AGATCT [GA]^GATC[CT]
XmaCI 208 CCCGGG C^CCGGG
XmaI 208 CCCGGG C^CCGGG
XmnI 2134 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 1202 GTATAC GT^[AC][GT]AC
2914 GTATAC
AcyI 172 GGCGCC G[GA]^CG[CT]C
277 GACGCC
AflIII 408 ACATGT A^C[GA][CT]GT
852 ACATGT
AlwI 463 GGATC GGATC(4/5)
1939 GGATC
BanI 172 GGCGCC G^G[CT][GA]CC
1721 GGTGCC
BbvCI 1252 CCTCAGC CCTCAGC(-5/-2)
253 GCTGAGG
BsaHI 172 GGCGCC G[GA]^CG[CT]C
277 GACGCC
Bsp1407I 1746 TGTACA T^GTACA
2604 TGTACA
BspLU11I 408 ACATGT A^CATGT
852 ACATGT
BsrDI 1497 CATTGC GCAATG(2/0)
3094 GCAATG
BsrGI 1746 TGTACA T^GTACA
2604 TGTACA
Bst1107I 1202 GTATAC GTA^TAC
2914 GTATAC
BstAPI 1882 GCACAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1891 GCAGCAGCTGC
BstZ17I 1202 GTATAC GTA^TAC
2914 GTATAC
EcoT14I 124 CCTAGG C^C[AT][AT]GG
2805 CCAAGG
HaeII 172 GGCGCC [GA]GCGC^[CT]
250 GGCGCT
Hin1I 172 GGCGCC G[GA]^CG[CT]C
277 GACGCC
KspI 132 CCGCGG CCGC^GG
342 CCGCGG
MfeI 638 CAATTG C^AATTG
3366 CAATTG
MunI 638 CAATTG C^AATTG
3366 CAATTG
NspI 408 ACATGT [GA]CATG^[CT]
852 ACATGT
PleI 368 GACTC GAGTC(4/5)
1135 GAGTC
PshAI 2002 GACCTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2176 GACTGAGGTC
PvuII 1895 CAGCTG CAG^CTG
1976 CAGCTG
SacII 132 CCGCGG CCGC^GG
342 CCGCGG
SspBI 1746 TGTACA T^GTACA
2604 TGTACA
StyI 124 CCTAGG C^C[AT][AT]GG
2805 CCAAGG
XcmI 597 CCATAGACAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1166 CCAACATTCCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwNI ApaI ApoI AspHI AvaI
AvaII BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EcoNI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PstI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SspI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I