##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AflIII AgeI AocI Aor51HI AscI
AseI AsnI Asp718I AviII AvrII
BamHI BbvCI BclI BfrI BglI
BlnI BlpI Bpu1102I BsaBI BsaWI
BseAI BsiWI BsmBI BspDI BspEI
BspHI BspLU11I BsrFI BssHII BssSI
Bst1107I BstBI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MamI MfeI MluI MroI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI SexAI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SwaI XbaI
XcmI XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 2284 GACGTC GACGT^C
AccII 545 CGCG CG^CG
AhdI 2284 GACGTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 524 GTGCAC G^TGCAC
ApaI 1581 GGGCCC GGGCC^C
ApaLI 524 GTGCAC G^TGCAC
Asp700I 2326 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2284 GACGTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1958 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1709 CCCGGG C^[CT]CG[GA]G
BalI 2048 TGGCCA TGG^CCA
BbeI 516 GGCGCC GGCGC^C
BciVI 1975 GGATAC GTATCC(6/5)
BsaI 1895 GAGACC GGTCTC(1/5)
BsgI 1508 CTGCAC GTGCAG(16/14)
BsiEI 1764 CGACCG CG[GA][CT]^CG
BsoBI 1709 CCCGGG C^[CT]CG[GA]G
BstAPI 1363 GCACTGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1727 GGTTACC G^GT[ACGT]ACC
BstPI 1727 GGTTACC G^GT[ACGT]ACC
BstUI 545 CGCG CG^CG
BstXI 586 CCATCAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Eam1105I 2284 GACGTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 26 CCTCTGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1727 GGTTACC G^GT[ACGT]ACC
HaeII 516 GGCGCC [GA]GCGC^[CT]
KasI 516 GGCGCC G^GCGCC
MluNI 2048 TGGCCA TGG^CCA
MscI 2048 TGGCCA TGG^CCA
MslI 2122 CACAAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 545 CGCG CG^CG
NarI 516 GGCGCC GG^CGCC
PflFI 1958 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 1416 GAGCTC GAGCT^C
SapI 1025 GCTCTTC GCTCTTC(1/4)
ScaI 949 AGTACT AGT^ACT
SfoI 516 GGCGCC GGC^GCC
SmaI 1709 CCCGGG CCC^GGG
SspI 283 AATATT AAT^ATT
StuI 1441 AGGCCT AGG^CCT
Tth111I 1958 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1709 CCCGGG C^CCGGG
XmaI 1709 CCCGGG C^CCGGG
XmnI 2326 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AlwI 809 GATCC GGATC(4/5)
1805 GATCC
BcgI 416 CGAAGGATTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
773 CGAGTCGGGTGC
BglII 186 AGATCT A^GATCT
2161 AGATCT
BsmI 2001 GAATGC GAATGC(1/-1)
2023 GAATGC
Bsp1407I 1717 TGTACA T^GTACA
2342 TGTACA
BsrDI 1301 CATTGC GCAATG(2/0)
90 GCAATG
BsrGI 1717 TGTACA T^GTACA
2342 TGTACA
DraIII 318 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1364 CACTGTGTG
DsaI 752 CCGTGG C^C[GA][CT]GG
795 CCGTGG
EaeI 1649 TGGCCG [CT]^GGCC[GA]
2048 TGGCCA
EcoRI 800 GAATTC G^AATTC
1272 GAATTC
EcoT14I 348 CCAAGG C^C[AT][AT]GG
1723 CCAAGG
HgaI 67 GACGC GACGC(5/10)
1602 GACGC
SspBI 1717 TGTACA T^GTACA
2342 TGTACA
StyI 348 CCAAGG C^C[AT][AT]GG
1723 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwNI ApoI AspHI AvaII BanI
BanII BbrPI BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaAI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrBI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PmaCI PmlI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII