##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
Acc65I AclI AgeI AhdI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BbeI
BcgI BciVI BcnI BlnI BsaBI
BsgI BsiEI BsiWI BspDI BspHI
BsrBI BsrFI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I Cfr10I ClaI
CpoI EagI Eam1105I EclXI Eco52I
EcoO65I EcoRV FseI FspI HindIII
HpaI KasI KpnI MamI MfeI
MluI MunI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SbfI ScaI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SwaI Tth111I XbaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 3018 GACGTC GACGT^C
AccI 1049 GTCGAC GT^[AC][GT]AC
AflII 1582 CTTAAG C^TTAAG
Alw44I 200 GTGCAC G^TGCAC
AocI 1054 CCTCAGG CC^T[ACGT]AGG
Aor51HI 16 AGCGCT AGC^GCT
ApaLI 200 GTGCAC G^TGCAC
BamHI 3035 GGATCC G^GATCC
BbrPI 2606 CACGTG CAC^GTG
BbvCI 895 CCTCAGC CCTCAGC(-5/-2)
BclI 639 TGATCA T^GATCA
BfrI 1582 CTTAAG C^TTAAG
BglII 1481 AGATCT A^GATCT
BlpI 67 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 67 GCTCAGC GC^T[ACGT]AGC
BsmI 697 GAATGC GAATGC(1/-1)
BssSI 1117 CACGAG CACGAG(-5/-1)
BstAPI 1153 GCAGGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1054 CCTCAGG CC^T[ACGT]AGG
CelII 67 GCTCAGC GC^T[ACGT]AGC
DraIII 2304 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 16 AGCGCT AGC^GCT
Eco81I 1054 CCTCAGG CC^T[ACGT]AGG
EcoNI 2393 CCTCCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 56 GAATTC G^AATTC
EcoT22I 1379 ATGCAT ATGCA^T
FbaI 639 TGATCA T^GATCA
HincII 1049 GTCGAC GT[CT]^[GA]AC
HindII 1049 GTCGAC GT[CT]^[GA]AC
KspI 1791 CCGCGG CCGC^GG
NcoI 452 CCATGG C^CATGG
NsiI 1379 ATGCAT ATGCA^T
PmaCI 2606 CACGTG CAC^GTG
PmlI 2606 CACGTG CAC^GTG
PpuMI 1514 AGGACCT [GA]G^G[AT]CC[CT]
SacI 440 GAGCTC GAGCT^C
SacII 1791 CCGCGG CCGC^GG
SalI 1049 GTCGAC G^TCGAC
SapI 2078 GCTCTTC GCTCTTC(1/4)
SexAI 1670 ACCTGGT A^CC[AT]GGT
SfiI 223 GGCCCACCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 273 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccIII 549 TCCGGA T^CCGGA
2271 TCCGGA
AcsI 56 GAATTC [GA]^AATT[CT]
1411 GAATTT
ApoI 56 GAATTC [GA]^AATT[CT]
1411 GAATTT
AvaI 1244 CCCGAG C^[CT]CG[GA]G
1657 CTCGGG
BglI 224 GCCCACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1232 GCCCGCATGGC
BsaAI 218 TACGTG [CT]AC^GT[GA]
2606 CACGTG
BsaWI 549 TCCGGA [AT]^CCGG[AT]
2271 TCCGGA
BseAI 549 TCCGGA T^CCGGA
2271 TCCGGA
BsmBI 1643 CGTCTC CGTCTC(1/5)
2794 CGTCTC
BsoBI 1244 CCCGAG C^[CT]CG[GA]G
1657 CTCGGG
Bsp1407I 432 TGTACA T^GTACA
3029 TGTACA
BspEI 549 TCCGGA T^CCGGA
2271 TCCGGA
BspLU11I 1089 ACATGT A^CATGT
3108 ACATGT
BsrDI 907 CATTGC GCAATG(2/0)
1762 CATTGC
BsrGI 432 TGTACA T^GTACA
3029 TGTACA
DraII 1514 AGGACCT [GA]G^G[ACGT]CC[CT]
1685 AGGCCCT
DrdI 1516 GACCTTTAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3012 GACGGTGACGTC
EcoO109I 1514 AGGACCT [GA]G^G[ACGT]CC[CT]
1685 AGGCCCT
HaeII 16 AGCGCT [GA]GCGC^[CT]
1156 GGCGCT
MroI 549 TCCGGA T^CCGGA
2271 TCCGGA
PshAI 1042 GACTGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2788 GACCCCCGTC
SspBI 432 TGTACA T^GTACA
3029 TGTACA
TaqI 1050 TCGA T^CGA
1176 TCGA
TthHB8I 1050 TCGA T^CGA
1176 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AspHI AvaII
BalI BanI BanII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DsaI EaeI
EarI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XcmI XhoII