##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AccI AclI AgeI Alw44I
ApaLI AscI AspI AviII AvrII
BamHI BbeI BbvCI BcgI BciVI
BlnI BpmI BsiWI BsmI BspDI
BspLU11I BspMI BsrBI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraIII EcoO65I EcoRI EcoT22I
FseI FspI HindIII HpaI KasI
MfeI MluI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI Psp1406I PvuI RsrII SalI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI StuI SwaI
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 2009 GGTACC G^GTACC
AccIII 1012 TCCGGA T^CCGGA
AcsI 973 AAATTT [GA]^AATT[CT]
AcyI 1314 GGCGTC G[GA]^CG[CT]C
AocI 618 CCTCAGG CC^T[ACGT]AGG
ApoI 973 AAATTT [GA]^AATT[CT]
Asp700I 1878 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2009 GGTACC G^GTACC
BclI 611 TGATCA T^GATCA
BglII 559 AGATCT A^GATCT
BsaBI 273 GATTATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1314 GGCGTC G[GA]^CG[CT]C
BsaWI 1012 TCCGGA [AT]^CCGG[AT]
BseAI 1012 TCCGGA T^CCGGA
BsiEI 78 CGGCCG CG[GA][CT]^CG
Bsp1407I 2111 TGTACA T^GTACA
BspEI 1012 TCCGGA T^CCGGA
BspHI 1434 TCATGA T^CATGA
BsrGI 2111 TGTACA T^GTACA
BssSI 248 CACGAG CACGAG(-5/-1)
BstAPI 1972 GCATCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 755 CCACTACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 618 CCTCAGG CC^T[ACGT]AGG
DraI 2342 TTTAAA TTT^AAA
DrdI 2157 GACTATTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 78 CGGCCG C^GGCCG
EclXI 78 CGGCCG C^GGCCG
Eco52I 78 CGGCCG C^GGCCG
Eco81I 618 CCTCAGG CC^T[ACGT]AGG
FbaI 611 TGATCA T^GATCA
Hin1I 1314 GGCGTC G[GA]^CG[CT]C
KpnI 2009 GGTACC GGTAC^C
MamI 273 GATTATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1012 TCCGGA T^CCGGA
NcoI 1345 CCATGG C^CATGG
NspI 2478 GCATGT [GA]CATG^[CT]
PpuMI 39 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 1434 TCATGA T^CATGA
SacI 1546 GAGCTC GAGCT^C
SapI 1065 GCTCTTC GCTCTTC(1/4)
SbfI 921 CCTGCAGG CCTGCA^GG
SexAI 188 ACCTGGT A^CC[AT]GGT
Sse8387I 921 CCTGCAGG CCTGCA^GG
SspBI 2111 TGTACA T^GTACA
SspI 1763 AATATT AAT^ATT
XmnI 1878 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AflII 1416 CTTAAG C^TTAAG
1815 CTTAAG
AhdI 187 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1893 GACAGGTTGTC
Aor51HI 170 AGCGCT AGC^GCT
706 AGCGCT
AseI 1841 ATTAAT AT^TAAT
2634 ATTAAT
AsnI 1841 ATTAAT AT^TAAT
2634 ATTAAT
AspEI 187 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1893 GACAGGTTGTC
AspHI 8 GTGCTC G[AT]GC[AT]^C
1546 GAGCTC
AvaII 40 GGACC G^G[AT]CC
536 GGACC
BbrPI 698 CACGTG CAC^GTG
815 CACGTG
BbsI 143 GTCTTC GAAGAC(2/6)
1901 GTCTTC
BfrI 1416 CTTAAG C^TTAAG
1815 CTTAAG
BpuAI 143 GTCTTC GAAGAC(2/6)
1901 GTCTTC
BsaI 185 GAGACC GGTCTC(1/5)
1201 GAGACC
BseRI 543 GAGGAG GAGGAG(10/8)
1395 GAGGAG
BsiHKAI 8 GTGCTC G[AT]GC[AT]^C
1546 GAGCTC
BsmBI 1104 GAGACG CGTCTC(1/5)
1516 GAGACG
BsrDI 750 CATTGC GCAATG(2/0)
2223 CATTGC
BsrFI 53 GCCGGC [GA]^CCGG[CT]
1249 GCCGGC
Cfr10I 53 GCCGGC [GA]^CCGG[CT]
1249 GCCGGC
Eam1105I 187 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1893 GACAGGTTGTC
Eco47III 170 AGCGCT AGC^GCT
706 AGCGCT
EcoNI 1701 CCTGGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1862 CCTCCGGGAGG
EcoRV 1438 GATATC GAT^ATC
1772 GATATC
HaeII 170 AGCGCT [GA]GCGC^[CT]
706 AGCGCT
KspI 45 CCGCGG CCGC^GG
836 CCGCGG
NaeI 53 GCCGGC GCC^GGC
1249 GCCGGC
NgoMIV 53 GCCGGC G^CCGGC
1249 GCCGGC
PflMI 13 CCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1657 CCAGAGGCTGG
PmaCI 698 CACGTG CAC^GTG
815 CACGTG
PmlI 698 CACGTG CAC^GTG
815 CACGTG
PshBI 1841 ATTAAT AT^TAAT
2634 ATTAAT
SacII 45 CCGCGG CCGC^GG
836 CCGCGG
SmaI 1636 CCCGGG CCC^GGG
2004 CCCGGG
SmlI 1416 CTTAAG C^T[CT][GA]AG
1815 CTTAAG
Van91I 13 CCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1657 CCAGAGGCTGG
XcmI 85 CCAGGGAGGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2055 CCAGAGAGTCCCTGG
XmaCI 1636 CCCGGG C^CCGGG
2004 CCCGGG
XmaI 1636 CCCGGG C^CCGGG
2004 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI ApaI AvaI BalI
BanI BanII BbvI BcnI BfaI
BglI BlpI BmyI Bpu1102I BsaAI
BsaJI BsgI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII