##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI Asp718I AspEI AspI
AviII AvrII BamHI BclI BfrI
BglI BlnI BseAI BsiWI BsmBI
BspDI BspEI BspLU11I BstBI Bsu36I
ClaI CpoI DraII DraIII DrdI
Eam1105I Eco47III Eco81I EcoO109I EcoRI
EcoRV FbaI FseI FspI HpaI
KpnI MluI MroI NdeI NheI
NruI NspV PacI PflFI PinAI
PpuMI Psp1406I RsrII SalI SbfI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspI SwaI
Tth111I XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 1648 GACGTC GACGT^C
ApaI 1902 GGGCCC GGGCC^C
AseI 2423 ATTAAT AT^TAAT
AsnI 2423 ATTAAT AT^TAAT
BbeI 135 GGCGCC GGCGC^C
BbvCI 2004 GCTGAGG CCTCAGC(-5/-2)
BglII 795 AGATCT A^GATCT
BsaBI 2209 GATTCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2529 TCCGGT [AT]^CCGG[AT]
BsmI 1358 GCATTC GAATGC(1/-1)
BspHI 972 TCATGA T^CATGA
BspMI 2186 GCAGGT ACCTGC(4/8)
BssHII 39 GCGCGC G^CGCGC
BssSI 463 CTCGTG CACGAG(-5/-1)
Bst1107I 2829 GTATAC GTA^TAC
BstEII 3321 GGTAACC G^GT[ACGT]ACC
BstPI 3321 GGTAACC G^GT[ACGT]ACC
BstXI 642 CCAGTTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2829 GTATAC GTA^TAC
EcoNI 2172 CCTGGCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3321 GGTAACC G^GT[ACGT]ACC
EcoT22I 244 ATGCAT ATGCA^T
KasI 135 GGCGCC G^GCGCC
KspI 80 CCGCGG CCGC^GG
MamI 2209 GATTCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3217 CAATTG C^AATTG
MunI 3217 CAATTG C^AATTG
NaeI 154 GCCGGC GCC^GGC
NarI 135 GGCGCC GG^CGCC
NgoMIV 154 GCCGGC G^CCGGC
NsiI 244 ATGCAT ATGCA^T
PaeR7I 1879 CTCGAG C^TCGAG
PflMI 1197 CCAAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmeI 3476 GTTTAAAC GTTT^AAAC
PshBI 2423 ATTAAT AT^TAAT
PstI 1037 CTGCAG CTGCA^G
PvuI 2214 CGATCG CGAT^CG
RcaI 972 TCATGA T^CATGA
SacII 80 CCGCGG CCGC^GG
SfoI 135 GGCGCC GGC^GCC
Van91I 1197 CCAAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2814 TCTAGA T^CTAGA
XhoI 1879 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 43 enzymes) #
###################################
AccI 637 GTAGAC GT^[AC][GT]AC
2829 GTATAC
AcyI 135 GGCGCC G[GA]^CG[CT]C
1648 GACGTC
Asp700I 316 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
319 GAAGTTCTTC
AvaI 1268 CCCGAG C^[CT]CG[GA]G
1879 CTCGAG
BalI 1967 TGGCCA TGG^CCA
2668 TGGCCA
BbrPI 1495 CACGTG CAC^GTG
2680 CACGTG
BcgI 165 CGAGGCTCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1029 CGAGACCTCTGC
BciVI 2747 GGATAC GTATCC(6/5)
345 GTATCC
BlpI 2 GCTAAGC GC^T[ACGT]AGC
205 GCTGAGC
Bpu1102I 2 GCTAAGC GC^T[ACGT]AGC
205 GCTGAGC
BsaAI 1495 CACGTG [CT]AC^GT[GA]
2680 CACGTG
BsaHI 135 GGCGCC G[GA]^CG[CT]C
1648 GACGTC
BsgI 1601 GTGCAG GTGCAG(16/14)
2982 GTGCAG
BsoBI 1268 CCCGAG C^[CT]CG[GA]G
1879 CTCGAG
Bsp1407I 3253 TGTACA T^GTACA
3468 TGTACA
BsrBI 526 GAGCGG CCGCTC(-3/-3)
1888 GAGCGG
BsrFI 154 GCCGGC [GA]^CCGG[CT]
3067 ACCGGC
BsrGI 3253 TGTACA T^GTACA
3468 TGTACA
BstAPI 175 GCAGCCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2231 GCAGCCGCTGC
CelII 2 GCTAAGC GC^T[ACGT]AGC
205 GCTGAGC
Cfr10I 154 GCCGGC [GA]^CCGG[CT]
3067 ACCGGC
EagI 83 CGGCCG C^GGCCG
3111 CGGCCG
EclXI 83 CGGCCG C^GGCCG
3111 CGGCCG
Eco52I 83 CGGCCG C^GGCCG
3111 CGGCCG
HaeII 135 GGCGCC [GA]GCGC^[CT]
147 GGCGCT
Hin1I 135 GGCGCC G[GA]^CG[CT]C
1648 GACGTC
HincII 1444 GTTGAC GT[CT]^[GA]AC
2547 GTCAAC
HindII 1444 GTTGAC GT[CT]^[GA]AC
2547 GTCAAC
HindIII 1211 AAGCTT A^AGCTT
3207 AAGCTT
MluNI 1967 TGGCCA TGG^CCA
2668 TGGCCA
MscI 1967 TGGCCA TGG^CCA
2668 TGGCCA
NotI 82 GCGGCCGC GC^GGCCGC
3110 GCGGCCGC
PmaCI 1495 CACGTG CAC^GTG
2680 CACGTG
PmlI 1495 CACGTG CAC^GTG
2680 CACGTG
PshAI 1291 GACCGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1918 GACTTCAGTC
SacI 1582 GAGCTC GAGCT^C
1659 GAGCTC
SapI 1885 GAAGAGC GCTCTTC(1/4)
2071 GAAGAGC
ScaI 329 AGTACT AGT^ACT
2291 AGTACT
SmlI 29 CTTGAG C^T[CT][GA]AG
1879 CTCGAG
SphI 617 GCATGC GCATG^C
1053 GCATGC
SspBI 3253 TGTACA T^GTACA
3468 TGTACA
StuI 1367 AGGCCT AGG^CCT
1875 AGGCCT
XmnI 316 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
319 GAAGTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DsaI EaeI EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII