##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
Aor51HI AscI Asp718I AspI AviII
AvrII BamHI BbrPI BcgI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaI BsiEI BsrBI BssSI
Bst1107I BstAPI BstBI BstZ17I CelII
CpoI DraI EagI EclXI Eco47III
Eco52I EcoRV FbaI FspI HpaI
KpnI MluI NdeI NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacI SalI SbfI
ScaI SfiI SfuI SgrAI SnaBI
SphI Sse8387I SspI StuI SwaI
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AatII 840 GACGTC GACGT^C
AccIII 279 TCCGGA T^CCGGA
AcsI 2334 GAATTC [GA]^AATT[CT]
AhdI 1336 GACAGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1413 GTGCAC G^TGCAC
ApaLI 1413 GTGCAC G^TGCAC
ApoI 2334 GAATTC [GA]^AATT[CT]
AseI 1092 ATTAAT AT^TAAT
AsnI 1092 ATTAAT AT^TAAT
AspEI 1336 GACAGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1305 CCCGGG C^[CT]CG[GA]G
BbeI 1557 GGCGCC GGCGC^C
BbvCI 299 GCTGAGG CCTCAGC(-5/-2)
BpmI 2407 CTCCAG CTGGAG(16/14)
BsaBI 692 GATATACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 279 TCCGGA T^CCGGA
BseRI 1876 GAGGAG GAGGAG(10/8)
BsiWI 61 CGTACG C^GTACG
BsoBI 1305 CCCGGG C^[CT]CG[GA]G
BspDI 1019 ATCGAT AT^CGAT
BspEI 279 TCCGGA T^CCGGA
BspHI 2009 TCATGA T^CATGA
BspLU11I 1938 ACATGT A^CATGT
BssHII 730 GCGCGC G^CGCGC
BstEII 2608 GGTCACC G^GT[ACGT]ACC
BstPI 2608 GGTCACC G^GT[ACGT]ACC
ClaI 1019 ATCGAT AT^CGAT
DraIII 2379 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1336 GACAGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2608 GGTCACC G^GT[ACGT]ACC
EcoRI 2334 GAATTC G^AATTC
EcoT22I 2310 ATGCAT ATGCA^T
FseI 5 GGCCGGCC GGCCGG^CC
HindIII 2053 AAGCTT A^AGCTT
KasI 1557 GGCGCC G^GCGCC
KspI 2207 CCGCGG CCGC^GG
MamI 692 GATATACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1438 CAATTG C^AATTG
MroI 279 TCCGGA T^CCGGA
MunI 1438 CAATTG C^AATTG
NarI 1557 GGCGCC GG^CGCC
NcoI 874 CCATGG C^CATGG
NsiI 2310 ATGCAT ATGCA^T
NspI 1938 ACATGT [GA]CATG^[CT]
PpuMI 581 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 2013 GACCCTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1092 ATTAAT AT^TAAT
RcaI 2009 TCATGA T^CATGA
SacII 2207 CCGCGG CCGC^GG
SapI 463 GCTCTTC GCTCTTC(1/4)
SexAI 1153 ACCAGGT A^CC[AT]GGT
SfoI 1557 GGCGCC GGC^GCC
SmaI 1305 CCCGGG CCC^GGG
XmaCI 1305 CCCGGG C^CCGGG
XmaI 1305 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AflIII 1938 ACATGT A^C[GA][CT]GT
2039 ACACGT
AlwNI 897 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1026 CAGTGTCTG
AocI 135 CCTAAGG CC^T[ACGT]AGG
179 CCTAAGG
ApaI 1523 GGGCCC GGGCC^C
2001 GGGCCC
Asp700I 74 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1364 GAAGTGCTTC
BalI 244 TGGCCA TGG^CCA
541 TGGCCA
BciVI 311 GGATAC GTATCC(6/5)
1311 GGATAC
BglI 10 GCCCCGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
530 GCCATACCGGC
BsaWI 279 TCCGGA [AT]^CCGG[AT]
1701 TCCGGT
BsgI 993 CTGCAC GTGCAG(16/14)
1782 CTGCAC
BsmI 2150 GAATGC GAATGC(1/-1)
570 GCATTC
BspMI 928 ACCTGC ACCTGC(4/8)
95 GCAGGT
BstYI 304 GGATCT [GA]^GATC[CT]
1762 AGATCC
Bsu36I 135 CCTAAGG CC^T[ACGT]AGG
179 CCTAAGG
DrdI 1452 GACGGCAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2594 GACCGAGGTGTC
Eco81I 135 CCTAAGG CC^T[ACGT]AGG
179 CCTAAGG
EcoNI 89 CCTCCCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1346 CCTGCTGAAGG
EcoT14I 874 CCATGG C^C[AT][AT]GG
1519 CCAAGG
HaeII 1557 GGCGCC [GA]GCGC^[CT]
2399 AGCGCC
HincII 630 GTCAAC GT[CT]^[GA]AC
2551 GTTGAC
HindII 630 GTCAAC GT[CT]^[GA]AC
2551 GTTGAC
MflI 304 GGATCT [GA]^GATC[CT]
1762 AGATCC
MluNI 244 TGGCCA TGG^CCA
541 TGGCCA
MscI 244 TGGCCA TGG^CCA
541 TGGCCA
NaeI 6 GCCGGC GCC^GGC
526 GCCGGC
NgoMIV 6 GCCGGC G^CCGGC
526 GCCGGC
PvuII 900 CAGCTG CAG^CTG
1196 CAGCTG
SmlI 2145 CTTGAG C^T[CT][GA]AG
2662 CTCAAG
SpeI 2291 ACTAGT A^CTAGT
2543 ACTAGT
StyI 874 CCATGG C^C[AT][AT]GG
1519 CCAAGG
XcmI 350 CCAGCCTTTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1162 CCAGGATGACCGTGG
XhoII 304 GGATCT [GA]^GATC[CT]
1762 AGATCC
XmnI 74 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1364 GAAGTGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaII BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpuAI BsaHI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI Bsp1286I
Bsp1407I BsrDI BsrFI BsrGI BsrI
BssKI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SspBI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I