################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## AatII Acc65I AccIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BbeI BbrPI BbvCI BcgI BclI BlnI BsaBI BseAI BsiWI Bsp1407I BspEI BspLU11I BspMI BsrFI BsrGI BssHII BssSI Bst1107I BstEII BstPI BstZ17I Cfr10I CpoI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoT22I FbaI FseI FspI KasI KpnI KspI MamI MluI MroI NaeI NarI NcoI NgoMIV NheI NotI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SgrAI SnaBI SpeI Sse8387I SspBI StuI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccI 1421 GTCTAC GT^[AC][GT]AC AclI 2396 AACGTT AA^CGTT AcyI 199 GGCGTC G[GA]^CG[CT]C AflII 2498 CTTAAG C^TTAAG AflIII 2896 ACACGT A^C[GA][CT]GT AlwNI 2342 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG BalI 802 TGGCCA TGG^CCA BfrI 2498 CTTAAG C^TTAAG BglI 2213 GCCAAATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 446 GCTCAGC GC^T[ACGT]AGC Bpu1102I 446 GCTCAGC GC^T[ACGT]AGC BsaAI 2897 CACGTA [CT]AC^GT[GA] BsaHI 199 GGCGTC G[GA]^CG[CT]C BsaI 3408 GAGACC GGTCTC(1/5) BsaWI 778 TCCGGT [AT]^CCGG[AT] BsgI 3396 GTGCAG GTGCAG(16/14) BsiEI 710 CGATCG CG[GA][CT]^CG BspDI 1622 ATCGAT AT^CGAT BspHI 700 TCATGA T^CATGA BsrBI 207 GAGCGG CCGCTC(-3/-3) BsrDI 1866 CATTGC GCAATG(2/0) BstAPI 3263 GCAAAACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 2352 CCATCTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 446 GCTCAGC GC^T[ACGT]AGC ClaI 1622 ATCGAT AT^CGAT DraI 3507 TTTAAA TTT^AAA EaeI 802 TGGCCA [CT]^GGCC[GA] EcoNI 2039 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 2867 GATATC GAT^ATC HaeII 108 AGCGCC [GA]GCGC^[CT] HgaI 200 GCGTC GACGC(5/10) Hin1I 199 GGCGTC G[GA]^CG[CT]C HincII 791 GTTAAC GT[CT]^[GA]AC HindII 791 GTTAAC GT[CT]^[GA]AC HpaI 791 GTTAAC GTT^AAC MluNI 802 TGGCCA TGG^CCA MscI 802 TGGCCA TGG^CCA NdeI 2379 CATATG CA^TATG NruI 423 TCGCGA TCG^CGA Psp1406I 2396 AACGTT AA^CGTT PvuI 710 CGATCG CGAT^CG PvuII 784 CAGCTG CAG^CTG RcaI 700 TCATGA T^CATGA ScaI 1819 AGTACT AGT^ACT ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AfaI 1820 GTAC GT^AC 3528 GTAC AocI 67 CCTCAGG CC^T[ACGT]AGG 754 CCTCAGG AspHI 1920 GAGCTC G[AT]GC[AT]^C 2551 GAGCTC AvaII 1847 GGTCC G^G[AT]CC 2003 GGACC BamHI 1588 GGATCC G^GATCC 2326 GGATCC BanI 3 GGTGCC G^G[CT][GA]CC 78 GGTGCC BanII 1920 GAGCTC G[GA]GC[CT]^C 2551 GAGCTC BciVI 1965 GGATAC GTATCC(6/5) 2586 GTATCC BglII 1288 AGATCT A^GATCT 2203 AGATCT BpmI 16 CTGGAG CTGGAG(16/14) 2405 CTGGAG BsiHKAI 1920 GAGCTC G[AT]GC[AT]^C 2551 GAGCTC BsmBI 1379 CGTCTC CGTCTC(1/5) 2576 CGTCTC BsmFI 2002 GGGAC GGGAC(10/14) 21 GTCCC BsmI 671 GAATGC GAATGC(1/-1) 3401 GCATTC BstBI 1233 TTCGAA TT^CGAA 1803 TTCGAA Bsu36I 67 CCTCAGG CC^T[ACGT]AGG 754 CCTCAGG Eco81I 67 CCTCAGG CC^T[ACGT]AGG 754 CCTCAGG EcoT14I 2023 CCTTGG C^C[AT][AT]GG 3428 CCAAGG HindIII 842 AAGCTT A^AGCTT 2683 AAGCTT MslI 1574 CACTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 3449 CATCCCTGTG MspA1I 784 CAGCTG C[AC]G^C[GT]G 2805 CAGCGG NspV 1233 TTCGAA TT^CGAA 1803 TTCGAA PflMI 2018 CCACCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2752 CCAGTCTTTGG PpuMI 1846 AGGTCCT [GA]G^G[AT]CC[CT] 2002 GGGACCT RsaI 1820 GTAC GT^AC 3528 GTAC SacI 1920 GAGCTC GAGCT^C 2551 GAGCTC SfaNI 2637 GCATC GCATC(5/9) 2948 GCATC SfuI 1233 TTCGAA TT^CGAA 1803 TTCGAA SphI 1185 GCATGC GCATG^C 2373 GCATGC SspI 869 AATATT AAT^ATT 1530 AATATT StyI 2023 CCTTGG C^C[AT][AT]GG 3428 CCAAGG Van91I 2018 CCACCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2752 CCAGTCTTTGG XbaI 1069 TCTAGA T^CTAGA 3376 TCTAGA XcmI 579 CCAAACGTGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 805 CCATGAAGGTCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 88 enzymes) # ######################################### AccII AciI AcsI AluI AlwI ApoI Asp700I AvaI BbsI BbvI BcnI BfaI BmyI BpuAI BsaJI BseRI BsiYI BslI BsmAI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRI EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MfeI MflI MnlI MseI MspI MunI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI Sau3AI Sau96I ScrFI SfcI SmaI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmaCI XmaI XmnI