##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BbeI BbrPI BbvCI
BcgI BclI BlnI BsaBI BseAI
BsiWI Bsp1407I BspEI BspLU11I BspMI
BsrFI BsrGI BssHII BssSI Bst1107I
BstEII BstPI BstZ17I Cfr10I CpoI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoT22I
FbaI FseI FspI KasI KpnI
KspI MamI MluI MroI NaeI
NarI NcoI NgoMIV NheI NotI
NsiI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SgrAI SnaBI
SpeI Sse8387I SspBI StuI SwaI
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 1421 GTCTAC GT^[AC][GT]AC
AclI 2396 AACGTT AA^CGTT
AcyI 199 GGCGTC G[GA]^CG[CT]C
AflII 2498 CTTAAG C^TTAAG
AflIII 2896 ACACGT A^C[GA][CT]GT
AlwNI 2342 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
BalI 802 TGGCCA TGG^CCA
BfrI 2498 CTTAAG C^TTAAG
BglI 2213 GCCAAATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 446 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 446 GCTCAGC GC^T[ACGT]AGC
BsaAI 2897 CACGTA [CT]AC^GT[GA]
BsaHI 199 GGCGTC G[GA]^CG[CT]C
BsaI 3408 GAGACC GGTCTC(1/5)
BsaWI 778 TCCGGT [AT]^CCGG[AT]
BsgI 3396 GTGCAG GTGCAG(16/14)
BsiEI 710 CGATCG CG[GA][CT]^CG
BspDI 1622 ATCGAT AT^CGAT
BspHI 700 TCATGA T^CATGA
BsrBI 207 GAGCGG CCGCTC(-3/-3)
BsrDI 1866 CATTGC GCAATG(2/0)
BstAPI 3263 GCAAAACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2352 CCATCTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 446 GCTCAGC GC^T[ACGT]AGC
ClaI 1622 ATCGAT AT^CGAT
DraI 3507 TTTAAA TTT^AAA
EaeI 802 TGGCCA [CT]^GGCC[GA]
EcoNI 2039 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2867 GATATC GAT^ATC
HaeII 108 AGCGCC [GA]GCGC^[CT]
HgaI 200 GCGTC GACGC(5/10)
Hin1I 199 GGCGTC G[GA]^CG[CT]C
HincII 791 GTTAAC GT[CT]^[GA]AC
HindII 791 GTTAAC GT[CT]^[GA]AC
HpaI 791 GTTAAC GTT^AAC
MluNI 802 TGGCCA TGG^CCA
MscI 802 TGGCCA TGG^CCA
NdeI 2379 CATATG CA^TATG
NruI 423 TCGCGA TCG^CGA
Psp1406I 2396 AACGTT AA^CGTT
PvuI 710 CGATCG CGAT^CG
PvuII 784 CAGCTG CAG^CTG
RcaI 700 TCATGA T^CATGA
ScaI 1819 AGTACT AGT^ACT
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AfaI 1820 GTAC GT^AC
3528 GTAC
AocI 67 CCTCAGG CC^T[ACGT]AGG
754 CCTCAGG
AspHI 1920 GAGCTC G[AT]GC[AT]^C
2551 GAGCTC
AvaII 1847 GGTCC G^G[AT]CC
2003 GGACC
BamHI 1588 GGATCC G^GATCC
2326 GGATCC
BanI 3 GGTGCC G^G[CT][GA]CC
78 GGTGCC
BanII 1920 GAGCTC G[GA]GC[CT]^C
2551 GAGCTC
BciVI 1965 GGATAC GTATCC(6/5)
2586 GTATCC
BglII 1288 AGATCT A^GATCT
2203 AGATCT
BpmI 16 CTGGAG CTGGAG(16/14)
2405 CTGGAG
BsiHKAI 1920 GAGCTC G[AT]GC[AT]^C
2551 GAGCTC
BsmBI 1379 CGTCTC CGTCTC(1/5)
2576 CGTCTC
BsmFI 2002 GGGAC GGGAC(10/14)
21 GTCCC
BsmI 671 GAATGC GAATGC(1/-1)
3401 GCATTC
BstBI 1233 TTCGAA TT^CGAA
1803 TTCGAA
Bsu36I 67 CCTCAGG CC^T[ACGT]AGG
754 CCTCAGG
Eco81I 67 CCTCAGG CC^T[ACGT]AGG
754 CCTCAGG
EcoT14I 2023 CCTTGG C^C[AT][AT]GG
3428 CCAAGG
HindIII 842 AAGCTT A^AGCTT
2683 AAGCTT
MslI 1574 CACTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3449 CATCCCTGTG
MspA1I 784 CAGCTG C[AC]G^C[GT]G
2805 CAGCGG
NspV 1233 TTCGAA TT^CGAA
1803 TTCGAA
PflMI 2018 CCACCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2752 CCAGTCTTTGG
PpuMI 1846 AGGTCCT [GA]G^G[AT]CC[CT]
2002 GGGACCT
RsaI 1820 GTAC GT^AC
3528 GTAC
SacI 1920 GAGCTC GAGCT^C
2551 GAGCTC
SfaNI 2637 GCATC GCATC(5/9)
2948 GCATC
SfuI 1233 TTCGAA TT^CGAA
1803 TTCGAA
SphI 1185 GCATGC GCATG^C
2373 GCATGC
SspI 869 AATATT AAT^ATT
1530 AATATT
StyI 2023 CCTTGG C^C[AT][AT]GG
3428 CCAAGG
Van91I 2018 CCACCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2752 CCAGTCTTTGG
XbaI 1069 TCTAGA T^CTAGA
3376 TCTAGA
XcmI 579 CCAAACGTGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
805 CCATGAAGGTCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
ApoI Asp700I AvaI BbsI BbvI
BcnI BfaI BmyI BpuAI BsaJI
BseRI BsiYI BslI BsmAI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRI EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MfeI
MflI MnlI MseI MspI MunI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
Sau3AI Sau96I ScrFI SfcI SmaI
SmlI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmaCI XmaI XmnI