##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccIII AflII AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BamHI BbrPI BbsI BbvCI
BcgI BclI BfrI BlnI BpuAI
BsaBI BseAI BsiWI BsmBI BspDI
BspEI BspLU11I BsrBI Bst1107I BstBI
BstZ17I Bsu36I ClaI CpoI DrdI
DsaI Eam1105I Eco47III Eco81I EcoNI
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HindIII HpaI KpnI
KspI MamI MfeI MluI MroI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PaeR7I PmaCI PmeI PmlI PshAI
PshBI PvuI RsrII SacII SbfI
ScaI SfiI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I StuI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
AccI 773 GTCGAC GT^[AC][GT]AC
AclI 574 AACGTT AA^CGTT
AcyI 51 GGCGCC G[GA]^CG[CT]C
AflIII 616 ACGTGT A^C[GA][CT]GT
AgeI 2723 ACCGGT A^CCGGT
ApaI 44 GGGCCC GGGCC^C
Asp700I 1420 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2182 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2424 TGGCCA TGG^CCA
BbeI 51 GGCGCC GGCGC^C
BsaAI 615 TACGTG [CT]AC^GT[GA]
BsaHI 51 GGCGCC G[GA]^CG[CT]C
BsaI 173 GGTCTC GGTCTC(1/5)
BsaWI 2723 ACCGGT [AT]^CCGG[AT]
BsgI 732 CTGCAC GTGCAG(16/14)
BsiEI 30 CGGCCG CG[GA][CT]^CG
BsmI 869 GAATGC GAATGC(1/-1)
Bsp1407I 517 TGTACA T^GTACA
BspHI 2124 TCATGA T^CATGA
BspMI 901 GCAGGT ACCTGC(4/8)
BsrGI 517 TGTACA T^GTACA
BssHII 98 GCGCGC G^CGCGC
BssSI 1064 CACGAG CACGAG(-5/-1)
BstAPI 2460 GCATTTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2567 GGTCACC G^GT[ACGT]ACC
BstPI 2567 GGTCACC G^GT[ACGT]ACC
BstXI 1925 CCAAGCCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 2990 TTTAAA TTT^AAA
DraIII 165 CACGCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 30 CGGCCG C^GGCCG
EclXI 30 CGGCCG C^GGCCG
Eco52I 30 CGGCCG C^GGCCG
EcoO65I 2567 GGTCACC G^GT[ACGT]ACC
EcoT14I 2018 CCAAGG C^C[AT][AT]GG
HaeII 51 GGCGCC [GA]GCGC^[CT]
HgaI 2231 GACGC GACGC(5/10)
Hin1I 51 GGCGCC G[GA]^CG[CT]C
HincII 773 GTCGAC GT[CT]^[GA]AC
HindII 773 GTCGAC GT[CT]^[GA]AC
KasI 51 GGCGCC G^GCGCC
MluNI 2424 TGGCCA TGG^CCA
MscI 2424 TGGCCA TGG^CCA
NarI 51 GGCGCC GG^CGCC
NspI 3143 GCATGT [GA]CATG^[CT]
PacI 763 TTAATTAA TTAAT^TAA
PflFI 2182 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2723 ACCGGT A^CCGGT
Psp1406I 574 AACGTT AA^CGTT
PvuII 1415 CAGCTG CAG^CTG
RcaI 2124 TCATGA T^CATGA
SalI 773 GTCGAC G^TCGAC
SapI 2152 GCTCTTC GCTCTTC(1/4)
SexAI 2220 ACCTGGT A^CC[AT]GGT
SfoI 51 GGCGCC GGC^GCC
SmaI 22 CCCGGG CCC^GGG
SspBI 517 TGTACA T^GTACA
StyI 2018 CCAAGG C^C[AT][AT]GG
Tth111I 2182 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1720 TCTAGA T^CTAGA
XcmI 226 CCACACCTGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 22 CCCGGG C^CCGGG
XmaI 22 CCCGGG C^CCGGG
XmnI 1420 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwNI 1154 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
2216 CAGGACCTG
AspHI 13 GAGCTC G[AT]GC[AT]^C
1779 GAGCTC
BciVI 2301 GGATAC GTATCC(6/5)
538 GTATCC
BglI 84 GCCCTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
111 GCCGGTGCGGC
BglII 1306 AGATCT A^GATCT
2561 AGATCT
BlpI 1599 GCTGAGC GC^T[ACGT]AGC
2455 GCTGAGC
Bpu1102I 1599 GCTGAGC GC^T[ACGT]AGC
2455 GCTGAGC
BseRI 151 CTCCTC GAGGAG(10/8)
3012 GAGGAG
BsiHKAI 13 GAGCTC G[AT]GC[AT]^C
1779 GAGCTC
BsrDI 1738 CATTGC GCAATG(2/0)
1396 GCAATG
BsrFI 111 GCCGGT [GA]^CCGG[CT]
2723 ACCGGT
BstYI 1306 AGATCT [GA]^GATC[CT]
2561 AGATCT
CelII 1599 GCTGAGC GC^T[ACGT]AGC
2455 GCTGAGC
Cfr10I 111 GCCGGT [GA]^CCGG[CT]
2723 ACCGGT
EaeI 30 CGGCCG [CT]^GGCC[GA]
2424 TGGCCA
MflI 1306 AGATCT [GA]^GATC[CT]
2561 AGATCT
MspA1I 55 CCGCTG C[AC]G^C[GT]G
1415 CAGCTG
PflMI 164 CCACGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2381 CCAATTTCTGG
SacI 13 GAGCTC GAGCT^C
1779 GAGCTC
SspI 561 AATATT AAT^ATT
3038 AATATT
Van91I 164 CCACGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2381 CCAATTTCTGG
XhoII 1306 AGATCT [GA]^GATC[CT]
2561 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AvaI AvaII BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I