##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
Alw44I AocI Aor51HI ApaLI AscI
Asp718I AviII AvrII BamHI BbeI
BbrPI BcgI BciVI BglII BlnI
BsaBI BsaHI BsgI BsiWI BspDI
BspHI BspLU11I BspMI BssHII Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I ClaI CpoI DrdI Eco47III
Eco81I EcoO65I EcoRV EcoT22I FseI
FspI Hin1I KasI KpnI MamI
MfeI MunI NarI NheI NotI
NruI NsiI NspV PacI PflMI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RcaI RsrII SalI SbfI
SexAI SfiI SfoI SfuI SmaI
SnaBI SphI Sse8387I SwaI TfiI
Van91I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 65 enzymes) #
###################################
AccIII 1423 TCCGGA T^CCGGA
AflIII 655 ACGCGT A^C[GA][CT]GT
AgeI 2385 ACCGGT A^CCGGT
AhdI 1958 GACAATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1984 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 597 GGGCCC GGGCC^C
AseI 2648 ATTAAT AT^TAAT
AsnI 2648 ATTAAT AT^TAAT
Asp700I 2268 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1958 GACAATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1789 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2283 CTCGAG C^[CT]CG[GA]G
BalI 1567 TGGCCA TGG^CCA
BanI 1815 GGTGCC G^G[CT][GA]CC
BbsI 1092 GAAGAC GAAGAC(2/6)
BbvCI 2052 CCTCAGC CCTCAGC(-5/-2)
BglI 472 GCCCTGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1596 GCTCAGC GC^T[ACGT]AGC
BpmI 2290 CTCCAG CTGGAG(16/14)
Bpu1102I 1596 GCTCAGC GC^T[ACGT]AGC
BpuAI 1092 GAAGAC GAAGAC(2/6)
BsaAI 534 TACGTG [CT]AC^GT[GA]
BseAI 1423 TCCGGA T^CCGGA
BsoBI 2283 CTCGAG C^[CT]CG[GA]G
BspEI 1423 TCCGGA T^CCGGA
BsrDI 2494 CATTGC GCAATG(2/0)
BssSI 1915 CACGAG CACGAG(-5/-1)
BstAPI 1983 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1118 GGATCT [GA]^GATC[CT]
CelII 1596 GCTCAGC GC^T[ACGT]AGC
DraII 541 GGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 1930 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1958 GACAATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1608 GAAGAG CTCTTC(1/4)
EcoNI 1704 CCTCCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 541 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 1059 GAATTC G^AATTC
HindIII 1202 AAGCTT A^AGCTT
HpaI 2373 GTTAAC GTT^AAC
Ksp632I 1608 GAAGAG CTCTTC(1/4)
KspI 211 CCGCGG CCGC^GG
MflI 1118 GGATCT [GA]^GATC[CT]
MluI 655 ACGCGT A^CGCGT
MluNI 1567 TGGCCA TGG^CCA
MroI 1423 TCCGGA T^CCGGA
MscI 1567 TGGCCA TGG^CCA
NcoI 520 CCATGG C^CATGG
NdeI 2277 CATATG CA^TATG
NspI 2242 GCATGT [GA]CATG^[CT]
PaeR7I 2283 CTCGAG C^TCGAG
PflFI 1789 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2385 ACCGGT A^CCGGT
PpuMI 541 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 2648 ATTAAT AT^TAAT
SacII 211 CCGCGG CCGC^GG
SapI 1608 GAAGAGC GCTCTTC(1/4)
ScaI 1884 AGTACT AGT^ACT
SgrAI 2384 CACCGGTG C[GA]^CCGG[CT]G
SpeI 1455 ACTAGT A^CTAGT
SspI 2476 AATATT AAT^ATT
StuI 252 AGGCCT AGG^CCT
Tth111I 1789 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2283 CTCGAG C^TCGAG
XhoII 1118 GGATCT [GA]^GATC[CT]
XmnI 2268 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BclI 631 TGATCA T^GATCA
2409 TGATCA
BsiEI 340 CGGCCG CG[GA][CT]^CG
592 CGGCCG
BsmI 973 GAATGC GAATGC(1/-1)
2705 GCATTC
Bsp1407I 920 TGTACA T^GTACA
2108 TGTACA
BsrBI 395 CCGCTC CCGCTC(-3/-3)
641 CCGCTC
BsrGI 920 TGTACA T^GTACA
2108 TGTACA
DsaI 211 CCGCGG C^C[GA][CT]GG
520 CCATGG
EagI 340 CGGCCG C^GGCCG
592 CGGCCG
EclXI 340 CGGCCG C^GGCCG
592 CGGCCG
Eco52I 340 CGGCCG C^GGCCG
592 CGGCCG
FbaI 631 TGATCA T^GATCA
2409 TGATCA
HaeII 1108 AGCGCC [GA]GCGC^[CT]
1612 AGCGCC
HincII 1925 GTCAAC GT[CT]^[GA]AC
2373 GTTAAC
HindII 1925 GTCAAC GT[CT]^[GA]AC
2373 GTTAAC
SacI 1257 GAGCTC GAGCT^C
1288 GAGCTC
SspBI 920 TGTACA T^GTACA
2108 TGTACA
Tsp45I 558 GTGAC ^GT[GC]AC
2297 GTCAC
XcmI 511 CCAGCTACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1791 CCAAGTCCTCGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AluI AlwI ApoI AspHI AvaII
BanII BbvI BcnI BfaI BfrI
BmyI BsaI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
EaeI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI Tru9I TseI Tsp509I TspRI
TthHB8I