##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AhdI Alw44I ApaLI AscI AseI
AsnI AspEI AspI AviII AvrII
BbvCI BcgI BfrI BlnI BsaBI
BseAI BsiWI BsmBI Bsp1407I BspDI
BspEI BspLU11I BsrDI BsrGI BstAPI
BstBI BstEII BstPI ClaI CpoI
DraI Eam1105I EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HindIII HpaI
MamI MfeI MluI MroI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SalI SfiI SfuI SgrAI SnaBI
SpeI SphI SspBI SspI StuI
SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 1825 GGTACC G^GTACC
AccI 1740 GTATAC GT^[AC][GT]AC
AcsI 1932 AAATTC [GA]^AATT[CT]
AgeI 1642 ACCGGT A^CCGGT
AlwNI 763 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 212 CCTGAGG CC^T[ACGT]AGG
Aor51HI 165 AGCGCT AGC^GCT
ApoI 1932 AAATTC [GA]^AATT[CT]
Asp700I 335 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1825 GGTACC G^GTACC
BamHI 1967 GGATCC G^GATCC
BbeI 2362 GGCGCC GGCGC^C
BbrPI 1669 CACGTG CAC^GTG
BciVI 714 GGATAC GTATCC(6/5)
BclI 682 TGATCA T^GATCA
BsaAI 1669 CACGTG [CT]AC^GT[GA]
BsaWI 1642 ACCGGT [AT]^CCGG[AT]
BspHI 679 TCATGA T^CATGA
BspMI 2038 ACCTGC ACCTGC(4/8)
BsrBI 2870 CCGCTC CCGCTC(-3/-3)
BssHII 196 GCGCGC G^CGCGC
Bst1107I 1740 GTATAC GTA^TAC
BstZ17I 1740 GTATAC GTA^TAC
Bsu36I 212 CCTGAGG CC^T[ACGT]AGG
DraIII 2779 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1175 GACCGCCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 165 AGCGCT AGC^GCT
Eco81I 212 CCTGAGG CC^T[ACGT]AGG
FbaI 682 TGATCA T^GATCA
HincII 2918 GTCAAC GT[CT]^[GA]AC
HindII 2918 GTCAAC GT[CT]^[GA]AC
KasI 2362 GGCGCC G^GCGCC
KpnI 1825 GGTACC GGTAC^C
KspI 84 CCGCGG CCGC^GG
MaeII 1670 ACGT A^CGT
NaeI 1269 GCCGGC GCC^GGC
NarI 2362 GGCGCC GG^CGCC
NgoMIV 1269 GCCGGC G^CCGGC
NspI 2813 GCATGT [GA]CATG^[CT]
PinAI 1642 ACCGGT A^CCGGT
PmaCI 1669 CACGTG CAC^GTG
PmlI 1669 CACGTG CAC^GTG
PstI 2040 CTGCAG CTGCA^G
RcaI 679 TCATGA T^CATGA
SacII 84 CCGCGG CCGC^GG
SbfI 2039 CCTGCAGG CCTGCA^GG
ScaI 865 AGTACT AGT^ACT
SexAI 2289 ACCTGGT A^CC[AT]GGT
SfoI 2362 GGCGCC GGC^GCC
SmlI 2534 CTCAAG C^T[CT][GA]AG
Sse8387I 2039 CCTGCAGG CCTGCA^GG
XcmI 1543 CCACCCCCACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 335 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 1664 GACGCC G[GA]^CG[CT]C
2362 GGCGCC
AvaI 141 CCCGGG C^[CT]CG[GA]G
190 CCCGGG
BbsI 1907 GAAGAC GAAGAC(2/6)
1221 GTCTTC
BfaI 2145 CTAG C^TAG
2219 CTAG
BglI 1428 GCCCTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1679 GCCCATGGGGC
BglII 1573 AGATCT A^GATCT
1624 AGATCT
BpuAI 1907 GAAGAC GAAGAC(2/6)
1221 GTCTTC
BsaHI 1664 GACGCC G[GA]^CG[CT]C
2362 GGCGCC
BsaI 456 GAGACC GGTCTC(1/5)
1173 GAGACC
BsoBI 141 CCCGGG C^[CT]CG[GA]G
190 CCCGGG
EagI 923 CGGCCG C^GGCCG
1271 CGGCCG
EclXI 923 CGGCCG C^GGCCG
1271 CGGCCG
Eco52I 923 CGGCCG C^GGCCG
1271 CGGCCG
EcoNI 1344 CCTGACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2561 CCTGGCACAGG
HgaI 148 GACGC GACGC(5/10)
1664 GACGC
Hin1I 1664 GACGCC G[GA]^CG[CT]C
2362 GGCGCC
MaeI 2145 CTAG C^TAG
2219 CTAG
MseI 1888 TTAA T^TAA
2299 TTAA
PpuMI 206 GGGTCCC [GA]G^G[AT]CC[CT]
2226 AGGACCC
PvuII 908 CAGCTG CAG^CTG
1082 CAGCTG
SacI 1500 GAGCTC GAGCT^C
1716 GAGCTC
SapI 2108 GAAGAGC GCTCTTC(1/4)
2474 GCTCTTC
SfcI 2040 CTGCAG C^T[GA][CT]AG
2262 CTACAG
SmaI 141 CCCGGG CCC^GGG
190 CCCGGG
TfiI 655 GAATC G^A[AT]TC
2303 GATTC
Tru9I 1888 TTAA T^TAA
2299 TTAA
XmaCI 141 CCCGGG C^CCGGG
190 CCCGGG
XmaI 141 CCCGGG C^CCGGG
190 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
ApaI AspHI AvaII BalI BanI
BanII BbvI BcnI BlpI BmyI
BpmI Bpu1102I BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BsrFI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PflMI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII