##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflII
AgeI AhdI Alw44I ApaLI AscI
Asp718I AspEI AspHI AspI AviII
AvrII BbeI BcgI BciVI BfrI
BglII BlnI BsaBI BsaHI BsaWI
BseAI BsiHKAI BsiWI BsmBI Bsp1407I
BspEI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstEII BstPI BstXI
BstZ17I CpoI DraIII DrdI Eam1105I
EcoO65I EcoRI EcoRV FspI Hin1I
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MroI MunI
NarI NotI NruI PacI PflFI
PinAI PmeI PvuI RsrII SacI
SacII SalI ScaI SexAI SfiI
SfoI SgrAI SnaBI SpeI SspBI
SspI SwaI Tth111I XcmI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccII 1053 CGCG CG^CG
AclI 728 AACGTT AA^CGTT
AflIII 1052 ACGCGT A^C[GA][CT]GT
ApaI 215 GGGCCC GGGCC^C
Asp700I 362 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 2665 GGCACC G^G[CT][GA]CC
BbrPI 2747 CACGTG CAC^GTG
BclI 691 TGATCA T^GATCA
BlpI 2099 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2099 GCTCAGC GC^T[ACGT]AGC
BsaI 3274 GGTCTC GGTCTC(1/5)
BsiEI 2813 CGGCCG CG[GA][CT]^CG
BspDI 1913 ATCGAT AT^CGAT
BspHI 3318 TCATGA T^CATGA
BsrFI 2811 GCCGGC [GA]^CCGG[CT]
BstUI 1053 CGCG CG^CG
CelII 2099 GCTCAGC GC^T[ACGT]AGC
Cfr10I 2811 GCCGGC [GA]^CCGG[CT]
ClaI 1913 ATCGAT AT^CGAT
EagI 2813 CGGCCG C^GGCCG
EclXI 2813 CGGCCG C^GGCCG
Eco52I 2813 CGGCCG C^GGCCG
EcoT14I 1893 CCATGG C^C[AT][AT]GG
EcoT22I 3362 ATGCAT ATGCA^T
FbaI 691 TGATCA T^GATCA
FseI 2810 GGCCGGCC GGCCGG^CC
MluI 1052 ACGCGT A^CGCGT
MvnI 1053 CGCG CG^CG
NaeI 2811 GCCGGC GCC^GGC
NcoI 1893 CCATGG C^CATGG
NdeI 915 CATATG CA^TATG
NgoMIV 2811 GCCGGC G^CCGGC
NheI 67 GCTAGC G^CTAGC
NsiI 3362 ATGCAT ATGCA^T
PaeR7I 462 CTCGAG C^TCGAG
PmaCI 2747 CACGTG CAC^GTG
PmlI 2747 CACGTG CAC^GTG
Psp1406I 728 AACGTT AA^CGTT
RcaI 3318 TCATGA T^CATGA
SapI 426 GCTCTTC GCTCTTC(1/4)
SbfI 1108 CCTGCAGG CCTGCA^GG
SphI 758 GCATGC GCATG^C
Sse8387I 1108 CCTGCAGG CCTGCA^GG
StuI 983 AGGCCT AGG^CCT
StyI 1893 CCATGG C^C[AT][AT]GG
XbaI 130 TCTAGA T^CTAGA
XhoI 462 CTCGAG C^TCGAG
XmnI 362 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AfaI 1129 GTAC GT^AC
3384 GTAC
AocI 1463 CCTCAGG CC^T[ACGT]AGG
1982 CCTGAGG
Aor51HI 55 AGCGCT AGC^GCT
975 AGCGCT
AseI 1472 ATTAAT AT^TAAT
3401 ATTAAT
AsnI 1472 ATTAAT AT^TAAT
3401 ATTAAT
BamHI 118 GGATCC G^GATCC
2336 GGATCC
BanII 215 GGGCCC G[GA]GC[CT]^C
2832 GGGCTC
BbsI 1198 GAAGAC GAAGAC(2/6)
2054 GAAGAC
BpuAI 1198 GAAGAC GAAGAC(2/6)
2054 GAAGAC
BsaAI 1739 CACGTA [CT]AC^GT[GA]
2747 CACGTG
BstAPI 1111 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2398 GCAGGACCTGC
BstBI 369 TTCGAA TT^CGAA
1428 TTCGAA
Bsu36I 1463 CCTCAGG CC^T[ACGT]AGG
1982 CCTGAGG
DraI 1538 TTTAAA TTT^AAA
3193 TTTAAA
DsaI 1860 CCGTGG C^C[GA][CT]GG
1893 CCATGG
Eco47III 55 AGCGCT AGC^GCT
975 AGCGCT
Eco81I 1463 CCTCAGG CC^T[ACGT]AGG
1982 CCTGAGG
EcoNI 2530 CCTGATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3249 CCTGGAGAAGG
FauI 1923 CCCGC CCCGC(4/6)
2490 GCGGG
HgaI 1000 GCGTC GACGC(5/10)
2043 GCGTC
HindIII 1421 AAGCTT A^AGCTT
2351 AAGCTT
NspV 369 TTCGAA TT^CGAA
1428 TTCGAA
PflMI 253 CCATGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1888 CCAGCCCATGG
PshAI 82 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2038 GACGAGCGTC
PshBI 1472 ATTAAT AT^TAAT
3401 ATTAAT
RsaI 1129 GTAC GT^AC
3384 GTAC
SfuI 369 TTCGAA TT^CGAA
1428 TTCGAA
SmaI 1728 CCCGGG CCC^GGG
2085 CCCGGG
Van91I 253 CCATGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1888 CCAGCCCATGG
XmaCI 1728 CCCGGG C^CCGGG
2085 CCCGGG
XmaI 1728 CCCGGG C^CCGGG
2085 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccI AciI AcsI AluI AlwI
AlwNI ApoI AvaI AvaII BalI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII