##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflIII
AgeI AhdI AocI Aor51HI ApaI
AscI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BcgI BglI
BlnI BsaAI BsaBI BsaHI BsaWI
BseAI BsiWI BsmBI BsmI BsoBI
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CfoI Cfr10I ClaI CpoI
DraII DraIII DrdI Eam1105I Eco47III
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I FseI FspI HaeII
HhaI Hin1I HinP1I KasI KpnI
MamI MluI MroI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PstI PvuI PvuII RcaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
StuI StyI SwaI Tth111I Van91I
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccI 1865 GTAGAC GT^[AC][GT]AC
AclI 1296 AACGTT AA^CGTT
AflII 1517 CTTAAG C^TTAAG
Alw44I 1497 GTGCAC G^TGCAC
AlwI 595 GGATC GGATC(4/5)
AlwNI 412 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1497 GTGCAC G^TGCAC
AseI 1407 ATTAAT AT^TAAT
AsnI 1407 ATTAAT AT^TAAT
AspHI 1497 GTGCAC G[AT]GC[AT]^C
BalI 1060 TGGCCA TGG^CCA
BanII 2328 GGGCTC G[GA]GC[CT]^C
BbsI 788 GTCTTC GAAGAC(2/6)
BciVI 340 GGATAC GTATCC(6/5)
BclI 2373 TGATCA T^GATCA
BcnI 138 CCCGG CC^[GC]GG
BfrI 1517 CTTAAG C^TTAAG
BglII 1231 AGATCT A^GATCT
BlpI 711 GCTAAGC GC^T[ACGT]AGC
BpmI 2319 CTGGAG CTGGAG(16/14)
Bpu1102I 711 GCTAAGC GC^T[ACGT]AGC
BpuAI 788 GTCTTC GAAGAC(2/6)
BsaI 2655 GAGACC GGTCTC(1/5)
BsiEI 39 CGGCCG CG[GA][CT]^CG
BsiHKAI 1497 GTGCAC G[AT]GC[AT]^C
BsiYI 77 CCCTGCTGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 77 CCCTGCTGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 142 GTCCC GGGAC(10/14)
Bsp1407I 1439 TGTACA T^GTACA
BsrDI 637 CATTGC GCAATG(2/0)
BsrGI 1439 TGTACA T^GTACA
BssSI 11 CTCGTG CACGAG(-5/-1)
CelII 711 GCTAAGC GC^T[ACGT]AGC
EagI 39 CGGCCG C^GGCCG
EclXI 39 CGGCCG C^GGCCG
Eco52I 39 CGGCCG C^GGCCG
EcoT22I 612 ATGCAT ATGCA^T
FbaI 2373 TGATCA T^GATCA
HapII 139 CCGG C^CGG
HgaI 114 GCGTC GACGC(5/10)
HindIII 2065 AAGCTT A^AGCTT
HpaI 2565 GTTAAC GTT^AAC
HpaII 139 CCGG C^CGG
HphI 1557 TCACC GGTGA(8/7)
MluNI 1060 TGGCCA TGG^CCA
MscI 1060 TGGCCA TGG^CCA
MspI 139 CCGG C^CGG
NciI 138 CCCGG CC^[GC]GG
NsiI 612 ATGCAT ATGCA^T
PacI 996 TTAATTAA TTAAT^TAA
PshBI 1407 ATTAAT AT^TAAT
Psp1406I 1296 AACGTT AA^CGTT
SpeI 584 ACTAGT A^CTAGT
SspBI 1439 TGTACA T^GTACA
XbaI 2367 TCTAGA T^CTAGA
XcmI 1063 CCAGTGCTAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AvaII 141 GGTCC G^G[AT]CC
2386 GGACC
BbvI 922 GCAGC GCAGC(8/12)
81 GCTGC
BsgI 1819 GTGCAG GTGCAG(16/14)
2239 GTGCAG
BstF5I 615 CATCC GGATG(2/0)
2253 GGATG
BstYI 595 GGATCT [GA]^GATC[CT]
1231 AGATCT
DsaI 33 CCGCGG C^C[GA][CT]GG
49 CCGCGG
EaeI 39 CGGCCG [CT]^GGCC[GA]
1060 TGGCCA
FauI 32 CCCGC CCCGC(4/6)
111 CCCGC
FokI 615 CATCC GGATG(9/13)
2253 GGATG
HincII 257 GTTGAC GT[CT]^[GA]AC
2565 GTTAAC
HindII 257 GTTGAC GT[CT]^[GA]AC
2565 GTTAAC
KspI 33 CCGCGG CCGC^GG
49 CCGCGG
MaeII 1297 ACGT A^CGT
2022 ACGT
MfeI 298 CAATTG C^AATTG
1784 CAATTG
MflI 595 GGATCT [GA]^GATC[CT]
1231 AGATCT
MslI 1369 CATTTCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2195 CACGATTATG
MunI 298 CAATTG C^AATTG
1784 CAATTG
SacII 33 CCGCGG CCGC^GG
49 CCGCGG
TaqI 1965 TCGA T^CGA
2674 TCGA
TseI 81 GCTGC G^C[AT]GC
922 GCAGC
Tsp45I 1083 GTCAC ^GT[GC]AC
1752 GTGAC
TthHB8I 1965 TCGA T^CGA
2674 TCGA
XhoII 595 GGATCT [GA]^GATC[CT]
1231 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI BfaI BmyI BsaJI BseRI
BsmAI Bsp1286I BsrI BssKI BstNI
BstUI Cac8I Cfr13I DdeI DpnII
DraI EarI EcoRII Fnu4HI HaeIII
HinfI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MspA1I
MvaI MvnI MwoI NdeII NlaIII
NlaIV NspI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI TfiI Tru9I Tsp509I TspRI