##################################
# NO CUT ENZYMES (  132 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        Asp718I     AspEI       AspHI       AviII       
BanI        BanII       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BfrI        BglI        
BlpI        BmyI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmBI       Bsp1286I    Bsp1407I    BspDI       
BspLU11I    BsrBI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRV       EcoT22I     
FbaI        FseI        FspI        HaeII       HgaI        
Hin1I       KasI        KpnI        KspI        MamI        
MfeI        MluI        MspA1I      MunI        MvnI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       Psp1406I    PvuI        PvuII       RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SphI        Sse8387I    SspBI       
StuI        SwaI        Van91I      XbaI        XcmI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AccIII        288  TCCGGA               T^CCGGA
AfaI         1216  GTAC                 GT^AC
AlwNI         725  CAGCAACTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I      1394  GAAATTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         2659  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         1347  CTCGAG               C^[CT]CG[GA]G
BalI          732  TGGCCA               TGG^CCA
BamHI        2611  GGATCC               G^GATCC
BbsI          828  GAAGAC               GAAGAC(2/6)
BcnI         2556  CCGGG                CC^[GC]GG
BglII         820  AGATCT               A^GATCT
BpmI           42  CTGGAG               CTGGAG(16/14)
BpuAI         828  GAAGAC               GAAGAC(2/6)
BsaI         2657  GAGACC               GGTCTC(1/5)
BseAI         288  TCCGGA               T^CCGGA
BsmI         1458  GAATGC               GAATGC(1/-1)
BsoBI        1347  CTCGAG               C^[CT]CG[GA]G
BspEI         288  TCCGGA               T^CCGGA
BspHI        1258  TCATGA               T^CATGA
BstAPI       1408  GCAATATTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI         2342  GCGC                 GCG^C
EaeI          732  TGGCCA               [CT]^GGCC[GA]
HhaI         2342  GCGC                 GCG^C
HinP1I       2342  GCGC                 G^CGC
MluNI         732  TGGCCA               TGG^CCA
MroI          288  TCCGGA               T^CCGGA
MscI          732  TGGCCA               TGG^CCA
NciI         2556  CCGGG                CC^[GC]GG
PaeR7I       1347  CTCGAG               C^TCGAG
PflFI        2659  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI        1443  AGGTCCT              [GA]G^G[AT]CC[CT]
RcaI         1258  TCATGA               T^CATGA
RsaI         1216  GTAC                 GT^AC
SexAI          94  ACCTGGT              A^CC[AT]GGT
TaqI         1348  TCGA                 T^CGA
Tth111I      2659  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I      1348  TCGA                 T^CGA
XhoI         1347  CTCGAG               C^TCGAG
XmnI         1394  GAAATTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AseI           19  ATTAAT               AT^TAAT
             1942  ATTAAT              
AsnI           19  ATTAAT               AT^TAAT
             1942  ATTAAT              
AvrII          56  CCTAGG               C^CTAGG
             2549  CCTAGG              
BlnI           56  CCTAGG               C^CTAGG
             2549  CCTAGG              
BsaWI         288  TCCGGA               [AT]^CCGG[AT]
              711  TCCGGT              
BspMI        1607  ACCTGC               ACCTGC(4/8)
             1734  ACCTGC              
BstXI          92  CCACCTGGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              428  CCACCTGGATGG        
DraII          52  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             1443  AGGTCCT             
DsaI         1044  CCATGG               C^C[GA][CT]GG
             1057  CCATGG              
EarI          442  GAAGAG               CTCTTC(1/4)
             1573  GAAGAG              
EcoO109I       52  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             1443  AGGTCCT             
EcoRI         582  GAATTC               G^AATTC
             2416  GAATTC              
HindIII      1371  AAGCTT               A^AGCTT
             2249  AAGCTT              
HpaI         2511  GTTAAC               GTT^AAC
             2544  GTTAAC              
Ksp632I       442  GAAGAG               CTCTTC(1/4)
             1573  GAAGAG              
NcoI         1044  CCATGG               C^CATGG
             1057  CCATGG              
NlaIV        1604  GGAACC               GG[ACGT]^[ACGT]CC
             2611  GGATCC              
NspI         1492  ACATGC               [GA]CATG^[CT]
             2166  ACATGC              
PshBI          19  ATTAAT               AT^TAAT
             1942  ATTAAT              
PstI         1589  CTGCAG               CTGCA^G
             2160  CTGCAG              
SfaNI        1427  GATGC                GCATC(5/9)
             2387  GCATC               
SpeI         2014  ACTAGT               A^CTAGT
             2079  ACTAGT              


#########################################
# 3 OR MORE CUT ENZYMES (   65 enzymes) #
#########################################

AciI        AcsI        AluI        AlwI        ApoI        
AvaII       BbvI        BfaI        BsaJI       BsiYI       
BslI        BsmAI       BsmFI       BsrDI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HincII      HindII      HinfI       HpaII       HphI        
ItaI        MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspI        MvaI        MwoI        NdeII       NlaIII      
PleI        Sau3AI      Sau96I      ScrFI       SfcI        
SmlI        SspI        StyI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       XhoII