##################################
# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspHI AviII
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BlpI BmyI Bpu1102I BsaAI BsaBI
BsaHI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I Bsp1407I BspDI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
Hin1I KasI KpnI KspI MamI
MfeI MluI MspA1I MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
StuI SwaI Van91I XbaI XcmI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccIII 288 TCCGGA T^CCGGA
AfaI 1216 GTAC GT^AC
AlwNI 725 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 1394 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2659 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1347 CTCGAG C^[CT]CG[GA]G
BalI 732 TGGCCA TGG^CCA
BamHI 2611 GGATCC G^GATCC
BbsI 828 GAAGAC GAAGAC(2/6)
BcnI 2556 CCGGG CC^[GC]GG
BglII 820 AGATCT A^GATCT
BpmI 42 CTGGAG CTGGAG(16/14)
BpuAI 828 GAAGAC GAAGAC(2/6)
BsaI 2657 GAGACC GGTCTC(1/5)
BseAI 288 TCCGGA T^CCGGA
BsmI 1458 GAATGC GAATGC(1/-1)
BsoBI 1347 CTCGAG C^[CT]CG[GA]G
BspEI 288 TCCGGA T^CCGGA
BspHI 1258 TCATGA T^CATGA
BstAPI 1408 GCAATATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 2342 GCGC GCG^C
EaeI 732 TGGCCA [CT]^GGCC[GA]
HhaI 2342 GCGC GCG^C
HinP1I 2342 GCGC G^CGC
MluNI 732 TGGCCA TGG^CCA
MroI 288 TCCGGA T^CCGGA
MscI 732 TGGCCA TGG^CCA
NciI 2556 CCGGG CC^[GC]GG
PaeR7I 1347 CTCGAG C^TCGAG
PflFI 2659 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1443 AGGTCCT [GA]G^G[AT]CC[CT]
RcaI 1258 TCATGA T^CATGA
RsaI 1216 GTAC GT^AC
SexAI 94 ACCTGGT A^CC[AT]GGT
TaqI 1348 TCGA T^CGA
Tth111I 2659 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1348 TCGA T^CGA
XhoI 1347 CTCGAG C^TCGAG
XmnI 1394 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AseI 19 ATTAAT AT^TAAT
1942 ATTAAT
AsnI 19 ATTAAT AT^TAAT
1942 ATTAAT
AvrII 56 CCTAGG C^CTAGG
2549 CCTAGG
BlnI 56 CCTAGG C^CTAGG
2549 CCTAGG
BsaWI 288 TCCGGA [AT]^CCGG[AT]
711 TCCGGT
BspMI 1607 ACCTGC ACCTGC(4/8)
1734 ACCTGC
BstXI 92 CCACCTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
428 CCACCTGGATGG
DraII 52 AGGCCCT [GA]G^G[ACGT]CC[CT]
1443 AGGTCCT
DsaI 1044 CCATGG C^C[GA][CT]GG
1057 CCATGG
EarI 442 GAAGAG CTCTTC(1/4)
1573 GAAGAG
EcoO109I 52 AGGCCCT [GA]G^G[ACGT]CC[CT]
1443 AGGTCCT
EcoRI 582 GAATTC G^AATTC
2416 GAATTC
HindIII 1371 AAGCTT A^AGCTT
2249 AAGCTT
HpaI 2511 GTTAAC GTT^AAC
2544 GTTAAC
Ksp632I 442 GAAGAG CTCTTC(1/4)
1573 GAAGAG
NcoI 1044 CCATGG C^CATGG
1057 CCATGG
NlaIV 1604 GGAACC GG[ACGT]^[ACGT]CC
2611 GGATCC
NspI 1492 ACATGC [GA]CATG^[CT]
2166 ACATGC
PshBI 19 ATTAAT AT^TAAT
1942 ATTAAT
PstI 1589 CTGCAG CTGCA^G
2160 CTGCAG
SfaNI 1427 GATGC GCATC(5/9)
2387 GCATC
SpeI 2014 ACTAGT A^CTAGT
2079 ACTAGT
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AvaII BbvI BfaI BsaJI BsiYI
BslI BsmAI BsmFI BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HincII HindII HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NdeII NlaIII
PleI Sau3AI Sau96I ScrFI SfcI
SmlI SspI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII