################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AviII BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I Bsp1407I BspDI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HgaI Hin1I KasI KpnI KspI MamI MfeI MluI MspA1I MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI StuI SwaI Van91I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccIII 288 TCCGGA T^CCGGA AfaI 1216 GTAC GT^AC AlwNI 725 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 1394 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 2659 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1347 CTCGAG C^[CT]CG[GA]G BalI 732 TGGCCA TGG^CCA BamHI 2611 GGATCC G^GATCC BbsI 828 GAAGAC GAAGAC(2/6) BcnI 2556 CCGGG CC^[GC]GG BglII 820 AGATCT A^GATCT BpmI 42 CTGGAG CTGGAG(16/14) BpuAI 828 GAAGAC GAAGAC(2/6) BsaI 2657 GAGACC GGTCTC(1/5) BseAI 288 TCCGGA T^CCGGA BsmI 1458 GAATGC GAATGC(1/-1) BsoBI 1347 CTCGAG C^[CT]CG[GA]G BspEI 288 TCCGGA T^CCGGA BspHI 1258 TCATGA T^CATGA BstAPI 1408 GCAATATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CfoI 2342 GCGC GCG^C EaeI 732 TGGCCA [CT]^GGCC[GA] HhaI 2342 GCGC GCG^C HinP1I 2342 GCGC G^CGC MluNI 732 TGGCCA TGG^CCA MroI 288 TCCGGA T^CCGGA MscI 732 TGGCCA TGG^CCA NciI 2556 CCGGG CC^[GC]GG PaeR7I 1347 CTCGAG C^TCGAG PflFI 2659 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 1443 AGGTCCT [GA]G^G[AT]CC[CT] RcaI 1258 TCATGA T^CATGA RsaI 1216 GTAC GT^AC SexAI 94 ACCTGGT A^CC[AT]GGT TaqI 1348 TCGA T^CGA Tth111I 2659 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1348 TCGA T^CGA XhoI 1347 CTCGAG C^TCGAG XmnI 1394 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AseI 19 ATTAAT AT^TAAT 1942 ATTAAT AsnI 19 ATTAAT AT^TAAT 1942 ATTAAT AvrII 56 CCTAGG C^CTAGG 2549 CCTAGG BlnI 56 CCTAGG C^CTAGG 2549 CCTAGG BsaWI 288 TCCGGA [AT]^CCGG[AT] 711 TCCGGT BspMI 1607 ACCTGC ACCTGC(4/8) 1734 ACCTGC BstXI 92 CCACCTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 428 CCACCTGGATGG DraII 52 AGGCCCT [GA]G^G[ACGT]CC[CT] 1443 AGGTCCT DsaI 1044 CCATGG C^C[GA][CT]GG 1057 CCATGG EarI 442 GAAGAG CTCTTC(1/4) 1573 GAAGAG EcoO109I 52 AGGCCCT [GA]G^G[ACGT]CC[CT] 1443 AGGTCCT EcoRI 582 GAATTC G^AATTC 2416 GAATTC HindIII 1371 AAGCTT A^AGCTT 2249 AAGCTT HpaI 2511 GTTAAC GTT^AAC 2544 GTTAAC Ksp632I 442 GAAGAG CTCTTC(1/4) 1573 GAAGAG NcoI 1044 CCATGG C^CATGG 1057 CCATGG NlaIV 1604 GGAACC GG[ACGT]^[ACGT]CC 2611 GGATCC NspI 1492 ACATGC [GA]CATG^[CT] 2166 ACATGC PshBI 19 ATTAAT AT^TAAT 1942 ATTAAT PstI 1589 CTGCAG CTGCA^G 2160 CTGCAG SfaNI 1427 GATGC GCATC(5/9) 2387 GCATC SpeI 2014 ACTAGT A^CTAGT 2079 ACTAGT ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI AvaII BbvI BfaI BsaJI BsiYI BslI BsmAI BsmFI BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NdeII NlaIII PleI Sau3AI Sau96I ScrFI SfcI SmlI SspI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII