##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AccIII AclI AflII AflIII AgeI
AscI AseI AsnI AspI AvrII
BamHI BbrPI BciVI BclI BfrI
BglII BlnI BsaAI BsaBI BseAI
BsiWI BspDI BspEI BspHI BspLU11I
Bst1107I BstBI BstEII BstPI BstYI
BstZ17I ClaI CpoI EcoO65I EcoRV
EcoT22I FbaI FseI HindIII HpaI
MamI MflI MluI MroI NheI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I RcaI RsrII SapI SbfI
ScaI SexAI SfiI SfuI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI TfiI Tth111I XbaI XhoII
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AatII 363 GACGTC GACGT^C
Acc65I 254 GGTACC G^GTACC
AcsI 1624 GAATTC [GA]^AATT[CT]
AhdI 1948 GACAACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2523 GTGCAC G^TGCAC
AlwI 249 GGATC GGATC(4/5)
AocI 1855 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1246 AGCGCT AGC^GCT
ApaLI 2523 GTGCAC G^TGCAC
ApoI 1624 GAATTC [GA]^AATT[CT]
Asp700I 402 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 254 GGTACC G^GTACC
AspEI 1948 GACAACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 667 CTCGAG C^[CT]CG[GA]G
BbvCI 621 GCTGAGG CCTCAGC(-5/-2)
BcgI 2329 GCACTGACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 1249 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1249 GCTGAGC GC^T[ACGT]AGC
BsaI 841 GAGACC GGTCTC(1/5)
BseRI 1366 CTCCTC GAGGAG(10/8)
BsmI 1047 GAATGC GAATGC(1/-1)
BsoBI 667 CTCGAG C^[CT]CG[GA]G
BspMI 1439 ACCTGC ACCTGC(4/8)
BssHII 1345 GCGCGC G^CGCGC
BssSI 1122 CACGAG CACGAG(-5/-1)
Bsu36I 1855 CCTGAGG CC^T[ACGT]AGG
CelII 1249 GCTGAGC GC^T[ACGT]AGC
DraI 2380 TTTAAA TTT^AAA
DraIII 2526 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2458 GACAAGAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1662 CGGCCG C^GGCCG
Eam1105I 1948 GACAACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 2137 CTCTTC CTCTTC(1/4)
EclXI 1662 CGGCCG C^GGCCG
Eco47III 1246 AGCGCT AGC^GCT
Eco52I 1662 CGGCCG C^GGCCG
Eco81I 1855 CCTGAGG CC^T[ACGT]AGG
EcoRI 1624 GAATTC G^AATTC
HincII 1945 GTCGAC GT[CT]^[GA]AC
HindII 1945 GTCGAC GT[CT]^[GA]AC
KpnI 254 GGTACC GGTAC^C
Ksp632I 2137 CTCTTC CTCTTC(1/4)
KspI 75 CCGCGG CCGC^GG
MaeII 364 ACGT A^CGT
NdeI 1325 CATATG CA^TATG
NotI 1661 GCGGCCGC GC^GGCCGC
NspI 989 ACATGC [GA]CATG^[CT]
PaeR7I 667 CTCGAG C^TCGAG
PvuI 1090 CGATCG CGAT^CG
SacI 1582 GAGCTC GAGCT^C
SacII 75 CCGCGG CCGC^GG
SalI 1945 GTCGAC G^TCGAC
SgrAI 7 CGCCGGCG C[GA]^CCGG[CT]G
XhoI 667 CTCGAG C^TCGAG
XmnI 402 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 1918 GTAGAC GT^[AC][GT]AC
1945 GTCGAC
AviII 980 TGCGCA TGC^GCA
1235 TGCGCA
BalI 1148 TGGCCA TGG^CCA
2343 TGGCCA
BbeI 5 GGCGCC GGCGC^C
166 GGCGCC
BbsI 1588 GTCTTC GAAGAC(2/6)
2809 GTCTTC
BglI 1274 GCCCGGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1892 GCCTCATGGGC
BpuAI 1588 GTCTTC GAAGAC(2/6)
2809 GTCTTC
BsaWI 1293 TCCGGT [AT]^CCGG[AT]
1922 ACCGGA
BsiEI 1090 CGATCG CG[GA][CT]^CG
1662 CGGCCG
BsmBI 387 CGTCTC CGTCTC(1/5)
562 GAGACG
BsrBI 997 GAGCGG CCGCTC(-3/-3)
1033 GAGCGG
BsrDI 433 GCAATG GCAATG(2/0)
2165 GCAATG
FspI 980 TGCGCA TGC^GCA
1235 TGCGCA
KasI 5 GGCGCC G^GCGCC
166 GGCGCC
MfeI 707 CAATTG C^AATTG
1571 CAATTG
MluNI 1148 TGGCCA TGG^CCA
2343 TGGCCA
MscI 1148 TGGCCA TGG^CCA
2343 TGGCCA
MseI 2381 TTAA T^TAA
2799 TTAA
MunI 707 CAATTG C^AATTG
1571 CAATTG
NaeI 8 GCCGGC GCC^GGC
356 GCCGGC
NarI 5 GGCGCC GG^CGCC
166 GGCGCC
NcoI 170 CCATGG C^CATGG
2154 CCATGG
NgoMIV 8 GCCGGC G^CCGGC
356 GCCGGC
PshAI 2611 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2725 GACAGGAGTC
SfoI 5 GGCGCC GGC^GCC
166 GGCGCC
SmlI 667 CTCGAG C^T[CT][GA]AG
1069 CTTGAG
StuI 554 AGGCCT AGG^CCT
1022 AGGCCT
Tru9I 2381 TTAA T^TAA
2799 TTAA
Tsp45I 1204 GTCAC ^GT[GC]AC
2594 GTGAC
XcmI 127 CCAACGCGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1982 CCAATGGCAAACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI ApaI AspHI AvaII BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaHI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BsrFI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspBI StyI
TaqI TseI Tsp509I TspRI TthHB8I
Van91I