##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AccI ApaI AscI
Asp718I AspI AvaI AviII BanII
BbrPI BcgI BciVI BclI BcnI
BglI BlpI Bpu1102I BsaAI BsaBI
BsiEI BsiWI BsmBI BsoBI BspDI
BspMI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I CelII ClaI
DraIII EagI EclXI Eco52I EcoNI
EcoO65I FbaI FseI FspI HindIII
KpnI KspI MamI NaeI NciI
NdeI NgoMIV NheI NotI NruI
NspV PaeR7I PflFI PmaCI PmeI
PmlI PshAI PvuI SacI SacII
SalI SbfI ScaI SfiI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
StuI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
AccIII 24 TCCGGA T^CCGGA
AclI 733 AACGTT AA^CGTT
AcyI 3300 GGCGCC G[GA]^CG[CT]C
AflII 2911 CTTAAG C^TTAAG
AgeI 2056 ACCGGT A^CCGGT
AhdI 2055 GACCGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2269 GTGCAC G^TGCAC
AlwNI 1867 CAGTAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 599 CCTGAGG CC^T[ACGT]AGG
Aor51HI 3256 AGCGCT AGC^GCT
ApaLI 2269 GTGCAC G^TGCAC
AspEI 2055 GACCGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 197 CCTAGG C^CTAGG
BalI 1735 TGGCCA TGG^CCA
BanI 3300 GGCGCC G^G[CT][GA]CC
BbeI 3300 GGCGCC GGCGC^C
BbvCI 2605 GCTGAGG CCTCAGC(-5/-2)
BfrI 2911 CTTAAG C^TTAAG
BlnI 197 CCTAGG C^CTAGG
BsaHI 3300 GGCGCC G[GA]^CG[CT]C
BsaI 2062 GGTCTC GGTCTC(1/5)
BseAI 24 TCCGGA T^CCGGA
BseRI 1537 GAGGAG GAGGAG(10/8)
BsmI 264 GAATGC GAATGC(1/-1)
BspEI 24 TCCGGA T^CCGGA
BspLU11I 531 ACATGT A^CATGT
BsrBI 8 GAGCGG CCGCTC(-3/-3)
BsrFI 2056 ACCGGT [GA]^CCGG[CT]
BssSI 90 CTCGTG CACGAG(-5/-1)
BstXI 2881 CCAGTCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 599 CCTGAGG CC^T[ACGT]AGG
Cfr10I 2056 ACCGGT [GA]^CCGG[CT]
CpoI 26 CGGACCG CG^G[AT]CCG
DraI 935 TTTAAA TTT^AAA
DraII 1755 AGGTCCT [GA]G^G[ACGT]CC[CT]
DrdI 1748 GACTAGTAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2163 CCATGG C^C[GA][CT]GG
EaeI 1735 TGGCCA [CT]^GGCC[GA]
Eam1105I 2055 GACCGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3256 AGCGCT AGC^GCT
Eco81I 599 CCTGAGG CC^T[ACGT]AGG
EcoO109I 1755 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 1329 GATATC GAT^ATC
FauI 2956 GCGGG CCCGC(4/6)
Hin1I 3300 GGCGCC G[GA]^CG[CT]C
KasI 3300 GGCGCC G^GCGCC
MluI 880 ACGCGT A^CGCGT
MluNI 1735 TGGCCA TGG^CCA
MroI 24 TCCGGA T^CCGGA
MscI 1735 TGGCCA TGG^CCA
NarI 3300 GGCGCC GG^CGCC
NcoI 2163 CCATGG C^CATGG
PacI 750 TTAATTAA TTAAT^TAA
PinAI 2056 ACCGGT A^CCGGT
PpuMI 1755 AGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 733 AACGTT AA^CGTT
RsrII 26 CGGACCG CG^G[AT]CCG
SapI 3241 GAAGAGC GCTCTTC(1/4)
SfoI 3300 GGCGCC GGC^GCC
SpeI 1749 ACTAGT A^CTAGT
SwaI 934 ATTTAAAT ATTT^AAAT
XbaI 2864 TCTAGA T^CTAGA
XcmI 2241 CCACCTGTATAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 38 CGCG CG^CG
881 CGCG
Asp700I 954 GAAACCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2752 GAAGATCTTC
BamHI 12 GGATCC G^GATCC
53 GGATCC
BglII 2754 AGATCT A^GATCT
3136 AGATCT
BpmI 5 CTGGAG CTGGAG(16/14)
356 CTGGAG
BsaWI 24 TCCGGA [AT]^CCGG[AT]
2056 ACCGGT
Bsp1407I 593 TGTACA T^GTACA
2538 TGTACA
BsrDI 663 GCAATG GCAATG(2/0)
1637 GCAATG
BsrGI 593 TGTACA T^GTACA
2538 TGTACA
BstUI 38 CGCG CG^CG
881 CGCG
CfoI 3257 GCGC GCG^C
3301 GCGC
EcoRI 501 GAATTC G^AATTC
3218 GAATTC
EcoT22I 643 ATGCAT ATGCA^T
3021 ATGCAT
HaeII 3256 AGCGCT [GA]GCGC^[CT]
3300 GGCGCC
HaeIII 551 GGCC GG^CC
1736 GGCC
HgaI 36 GACGC GACGC(5/10)
879 GACGC
HhaI 3257 GCGC GCG^C
3301 GCGC
HinP1I 3257 GCGC G^CGC
3301 GCGC
HincII 3007 GTTAAC GT[CT]^[GA]AC
3076 GTTAAC
HindII 3007 GTTAAC GT[CT]^[GA]AC
3076 GTTAAC
HpaI 3007 GTTAAC GTT^AAC
3076 GTTAAC
MaeII 734 ACGT A^CGT
2087 ACGT
MfeI 124 CAATTG C^AATTG
1531 CAATTG
MunI 124 CAATTG C^AATTG
1531 CAATTG
MvnI 38 CGCG CG^CG
881 CGCG
NsiI 643 ATGCAT ATGCA^T
3021 ATGCAT
PflMI 1155 CCAAGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3365 CCATGTTCTGG
SmlI 388 CTCAAG C^T[CT][GA]AG
2911 CTTAAG
SspBI 593 TGTACA T^GTACA
2538 TGTACA
SspI 745 AATATT AAT^ATT
2388 AATATT
Van91I 1155 CCAAGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3365 CCATGTTCTGG
XmnI 954 GAAACCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2752 GAAGATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AseI AsnI AspHI
AvaII BbsI BbvI BfaI BmyI
BpuAI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EarI
EcoRII EcoT14I Fnu4HI FokI HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PshBI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII