################################## # NO CUT ENZYMES ( 95 enzymes) # ################################## AatII Acc65I AccIII AcyI AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BamHI BbeI BbrPI BcgI BlnI BsaAI BsaHI BsaWI BseAI BsiWI Bsp1407I BspDI BspEI BsrBI BsrFI BsrGI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I Cfr10I ClaI CpoI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT22I FseI FspI Hin1I KasI KpnI MluI MroI NaeI NarI NgoMIV NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RsrII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SwaI Tth111I Van91I XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 2841 GTCTAC GT^[AC][GT]AC AclI 1523 AACGTT AA^CGTT AflIII 2883 ACATGT A^C[GA][CT]GT AhdI 657 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 1090 GATCC GGATC(4/5) AspEI 657 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BanI 574 GGTGCC G^G[CT][GA]CC BbsI 2196 GAAGAC GAAGAC(2/6) BbvCI 2671 CCTCAGC CCTCAGC(-5/-2) BciVI 3065 GGATAC GTATCC(6/5) BglI 76 GCCCGAACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 2196 GAAGAC GAAGAC(2/6) BsaI 1992 GGTCTC GGTCTC(1/5) BspHI 1059 TCATGA T^CATGA BspLU11I 2883 ACATGT A^CATGT BsrDI 2238 CATTGC GCAATG(2/0) BssHII 290 GCGCGC G^CGCGC BssSI 283 CACGAG CACGAG(-5/-1) Eam1105I 657 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 202 GAAGAG CTCTTC(1/4) EcoRV 2525 GATATC GAT^ATC FauI 197 GCGGG CCCGC(4/6) HgaI 300 GACGC GACGC(5/10) HindIII 444 AAGCTT A^AGCTT HpaI 1234 GTTAAC GTT^AAC Ksp632I 202 GAAGAG CTCTTC(1/4) KspI 62 CCGCGG CCGC^GG MaeII 1524 ACGT A^CGT NcoI 2555 CCATGG C^CATGG NdeI 2078 CATATG CA^TATG PaeR7I 295 CTCGAG C^TCGAG Psp1406I 1523 AACGTT AA^CGTT RcaI 1059 TCATGA T^CATGA SacII 62 CCGCGG CCGC^GG SapI 202 GAAGAGC GCTCTTC(1/4) ScaI 215 AGTACT AGT^ACT SmaI 2640 CCCGGG CCC^GGG SphI 936 GCATGC GCATG^C SspI 1984 AATATT AAT^ATT StuI 2371 AGGCCT AGG^CCT Tsp45I 1652 GTGAC ^GT[GC]AC XbaI 1086 TCTAGA T^CTAGA XcmI 561 CCAGTTTGCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 295 CTCGAG C^TCGAG XmaCI 2640 CCCGGG C^CCGGG XmaI 2640 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AvaI 295 CTCGAG C^[CT]CG[GA]G 2640 CCCGGG BalI 152 TGGCCA TGG^CCA 2580 TGGCCA BanII 235 GAGCTC G[GA]GC[CT]^C 2402 GAGCTC BsaBI 1084 GATCTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2733 GATTCAGATC BsiEI 264 CGGTCG CG[GA][CT]^CG 626 CGACCG BsmBI 298 GAGACG CGTCTC(1/5) 595 GAGACG BsmI 1692 GAATGC GAATGC(1/-1) 2821 GAATGC BsoBI 295 CTCGAG C^[CT]CG[GA]G 2640 CCCGGG BspMI 2274 ACCTGC ACCTGC(4/8) 2978 ACCTGC DraI 702 TTTAAA TTT^AAA 3167 TTTAAA DraII 655 GGGACCC [GA]G^G[ACGT]CC[CT] 2643 GGGACCC EaeI 152 TGGCCA [CT]^GGCC[GA] 2580 TGGCCA EcoO109I 655 GGGACCC [GA]G^G[ACGT]CC[CT] 2643 GGGACCC EcoT14I 815 CCAAGG C^C[AT][AT]GG 2555 CCATGG HaeII 93 GGCGCT [GA]GCGC^[CT] 2328 AGCGCC HapII 1126 CCGG C^CGG 2641 CCGG HincII 1234 GTTAAC GT[CT]^[GA]AC 2139 GTTGAC HindII 1234 GTTAAC GT[CT]^[GA]AC 2139 GTTGAC HpaII 1126 CCGG C^CGG 2641 CCGG MamI 1084 GATCTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2733 GATTCAGATC MluNI 152 TGGCCA TGG^CCA 2580 TGGCCA MscI 152 TGGCCA TGG^CCA 2580 TGGCCA MspI 1126 CCGG C^CGG 2641 CCGG NheI 493 GCTAGC G^CTAGC 1287 GCTAGC PpuMI 655 GGGACCC [GA]G^G[AT]CC[CT] 2643 GGGACCC PstI 761 CTGCAG CTGCA^G 1028 CTGCAG PvuII 124 CAGCTG CAG^CTG 2728 CAGCTG SacI 235 GAGCTC GAGCT^C 2402 GAGCTC StyI 815 CCAAGG C^C[AT][AT]GG 2555 CCATGG TfiI 1951 GAATC G^A[AT]TC 2733 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 87 enzymes) # ######################################### AccII AciI AcsI AfaI AflII AluI AlwNI ApoI AspHI AvaII BbvI BclI BcnI BfaI BfrI BglII BlpI BmyI BpmI Bpu1102I BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DsaI EcoRII FbaI Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI MaeI MaeIII MboI MboII MfeI MflI MnlI MseI MslI MspA1I MunI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI Tru9I TseI Tsp509I TspRI TthHB8I XhoII