##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspI AviII AvrII BamHI BbeI
BbrPI BcgI BlnI BsaAI BsaHI
BsaWI BseAI BsiWI Bsp1407I BspDI
BspEI BsrBI BsrFI BsrGI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraIII
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoT22I
FseI FspI Hin1I KasI KpnI
MluI MroI NaeI NarI NgoMIV
NotI NruI NsiI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI PvuI RsrII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SwaI Tth111I Van91I XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 2841 GTCTAC GT^[AC][GT]AC
AclI 1523 AACGTT AA^CGTT
AflIII 2883 ACATGT A^C[GA][CT]GT
AhdI 657 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1090 GATCC GGATC(4/5)
AspEI 657 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 574 GGTGCC G^G[CT][GA]CC
BbsI 2196 GAAGAC GAAGAC(2/6)
BbvCI 2671 CCTCAGC CCTCAGC(-5/-2)
BciVI 3065 GGATAC GTATCC(6/5)
BglI 76 GCCCGAACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 2196 GAAGAC GAAGAC(2/6)
BsaI 1992 GGTCTC GGTCTC(1/5)
BspHI 1059 TCATGA T^CATGA
BspLU11I 2883 ACATGT A^CATGT
BsrDI 2238 CATTGC GCAATG(2/0)
BssHII 290 GCGCGC G^CGCGC
BssSI 283 CACGAG CACGAG(-5/-1)
Eam1105I 657 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 202 GAAGAG CTCTTC(1/4)
EcoRV 2525 GATATC GAT^ATC
FauI 197 GCGGG CCCGC(4/6)
HgaI 300 GACGC GACGC(5/10)
HindIII 444 AAGCTT A^AGCTT
HpaI 1234 GTTAAC GTT^AAC
Ksp632I 202 GAAGAG CTCTTC(1/4)
KspI 62 CCGCGG CCGC^GG
MaeII 1524 ACGT A^CGT
NcoI 2555 CCATGG C^CATGG
NdeI 2078 CATATG CA^TATG
PaeR7I 295 CTCGAG C^TCGAG
Psp1406I 1523 AACGTT AA^CGTT
RcaI 1059 TCATGA T^CATGA
SacII 62 CCGCGG CCGC^GG
SapI 202 GAAGAGC GCTCTTC(1/4)
ScaI 215 AGTACT AGT^ACT
SmaI 2640 CCCGGG CCC^GGG
SphI 936 GCATGC GCATG^C
SspI 1984 AATATT AAT^ATT
StuI 2371 AGGCCT AGG^CCT
Tsp45I 1652 GTGAC ^GT[GC]AC
XbaI 1086 TCTAGA T^CTAGA
XcmI 561 CCAGTTTGCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 295 CTCGAG C^TCGAG
XmaCI 2640 CCCGGG C^CCGGG
XmaI 2640 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AvaI 295 CTCGAG C^[CT]CG[GA]G
2640 CCCGGG
BalI 152 TGGCCA TGG^CCA
2580 TGGCCA
BanII 235 GAGCTC G[GA]GC[CT]^C
2402 GAGCTC
BsaBI 1084 GATCTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2733 GATTCAGATC
BsiEI 264 CGGTCG CG[GA][CT]^CG
626 CGACCG
BsmBI 298 GAGACG CGTCTC(1/5)
595 GAGACG
BsmI 1692 GAATGC GAATGC(1/-1)
2821 GAATGC
BsoBI 295 CTCGAG C^[CT]CG[GA]G
2640 CCCGGG
BspMI 2274 ACCTGC ACCTGC(4/8)
2978 ACCTGC
DraI 702 TTTAAA TTT^AAA
3167 TTTAAA
DraII 655 GGGACCC [GA]G^G[ACGT]CC[CT]
2643 GGGACCC
EaeI 152 TGGCCA [CT]^GGCC[GA]
2580 TGGCCA
EcoO109I 655 GGGACCC [GA]G^G[ACGT]CC[CT]
2643 GGGACCC
EcoT14I 815 CCAAGG C^C[AT][AT]GG
2555 CCATGG
HaeII 93 GGCGCT [GA]GCGC^[CT]
2328 AGCGCC
HapII 1126 CCGG C^CGG
2641 CCGG
HincII 1234 GTTAAC GT[CT]^[GA]AC
2139 GTTGAC
HindII 1234 GTTAAC GT[CT]^[GA]AC
2139 GTTGAC
HpaII 1126 CCGG C^CGG
2641 CCGG
MamI 1084 GATCTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2733 GATTCAGATC
MluNI 152 TGGCCA TGG^CCA
2580 TGGCCA
MscI 152 TGGCCA TGG^CCA
2580 TGGCCA
MspI 1126 CCGG C^CGG
2641 CCGG
NheI 493 GCTAGC G^CTAGC
1287 GCTAGC
PpuMI 655 GGGACCC [GA]G^G[AT]CC[CT]
2643 GGGACCC
PstI 761 CTGCAG CTGCA^G
1028 CTGCAG
PvuII 124 CAGCTG CAG^CTG
2728 CAGCTG
SacI 235 GAGCTC GAGCT^C
2402 GAGCTC
StyI 815 CCAAGG C^C[AT][AT]GG
2555 CCATGG
TfiI 1951 GAATC G^A[AT]TC
2733 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AluI AlwNI ApoI AspHI AvaII
BbvI BclI BcnI BfaI BfrI
BglII BlpI BmyI BpmI Bpu1102I
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DsaI EcoRII FbaI
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI HphI ItaI MaeI MaeIII
MboI MboII MfeI MflI MnlI
MseI MslI MspA1I MunI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TaqI Tru9I TseI Tsp509I TspRI
TthHB8I XhoII