##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII AcsI AflII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspHI
BcgI BclI BfrI BglII BlpI
Bpu1102I BsiEI BsiHKAI BsiWI Bsp1407I
BspDI BspLU11I BsrGI BssHII BstAPI
BstBI BstEII BstPI CelII ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
FbaI FseI HindIII KspI MfeI
MluI MunI NdeI NheI NotI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI PvuI RsrII SacI
SacII SalI SapI ScaI SexAI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI SspBI SspI SwaI
Tsp509I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 1446 GGTACC G^GTACC
AccII 1257 CGCG CG^CG
AclI 1338 AACGTT AA^CGTT
AflIII 1889 ACGTGT A^C[GA][CT]GT
Asp718I 1446 GGTACC G^GTACC
AspI 1464 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1549 TGCGCA TGC^GCA
AvrII 1169 CCTAGG C^CTAGG
BamHI 1864 GGATCC G^GATCC
BbrPI 1781 CACGTG CAC^GTG
BciVI 1189 GTATCC GTATCC(6/5)
BlnI 1169 CCTAGG C^CTAGG
BsmBI 1530 GAGACG CGTCTC(1/5)
BsmI 127 GAATGC GAATGC(1/-1)
BspHI 2393 TCATGA T^CATGA
BsrFI 732 GCCGGC [GA]^CCGG[CT]
BssSI 1632 CTCGTG CACGAG(-5/-1)
Bst1107I 3820 GTATAC GTA^TAC
BstUI 1257 CGCG CG^CG
BstZ17I 3820 GTATAC GTA^TAC
Cfr10I 732 GCCGGC [GA]^CCGG[CT]
DrdI 2823 GACATCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 2477 GATATC GAT^ATC
FspI 1549 TGCGCA TGC^GCA
HincII 667 GTTAAC GT[CT]^[GA]AC
HindII 667 GTTAAC GT[CT]^[GA]AC
HpaI 667 GTTAAC GTT^AAC
KpnI 1446 GGTACC GGTAC^C
MvnI 1257 CGCG CG^CG
NaeI 732 GCCGGC GCC^GGC
NgoMIV 732 GCCGGC G^CCGGC
NruI 1256 TCGCGA TCG^CGA
PflFI 1464 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1781 CACGTG CAC^GTG
PmlI 1781 CACGTG CAC^GTG
Psp1406I 1338 AACGTT AA^CGTT
RcaI 2393 TCATGA T^CATGA
SbfI 1295 CCTGCAGG CCTGCA^GG
Sse8387I 1295 CCTGCAGG CCTGCA^GG
Tth111I 1464 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 3033 GTCTAC GT^[AC][GT]AC
3820 GTATAC
ApaI 467 GGGCCC GGGCC^C
3234 GGGCCC
BbeI 1622 GGCGCC GGCGC^C
1720 GGCGCC
BbvCI 1371 GCTGAGG CCTCAGC(-5/-2)
2126 GCTGAGG
BsaAI 1781 CACGTG [CT]AC^GT[GA]
1888 TACGTG
BsaBI 1502 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2636 GATCCCCATC
BsrBI 2066 CCGCTC CCGCTC(-3/-3)
3676 CCGCTC
BsrDI 132 CATTGC GCAATG(2/0)
1426 GCAATG
BstYI 1864 GGATCC [GA]^GATC[CT]
2635 AGATCC
DsaI 143 CCATGG C^C[GA][CT]GG
1801 CCATGG
EarI 2111 CTCTTC CTCTTC(1/4)
2943 GAAGAG
EcoT22I 129 ATGCAT ATGCA^T
4095 ATGCAT
KasI 1622 GGCGCC G^GCGCC
1720 GGCGCC
Ksp632I 2111 CTCTTC CTCTTC(1/4)
2943 GAAGAG
MamI 1502 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2636 GATCCCCATC
MflI 1864 GGATCC [GA]^GATC[CT]
2635 AGATCC
NarI 1622 GGCGCC GG^CGCC
1720 GGCGCC
NcoI 143 CCATGG C^CATGG
1801 CCATGG
NsiI 129 ATGCAT ATGCA^T
4095 ATGCAT
NspI 3387 GCATGT [GA]CATG^[CT]
4097 GCATGT
SfaNI 1507 GCATC GCATC(5/9)
3609 GCATC
SfoI 1622 GGCGCC GGC^GCC
1720 GGCGCC
XcmI 3091 CCATCCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3816 CCATGTATACACTGG
XhoII 1864 GGATCC [GA]^GATC[CT]
2635 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccIII AciI AcyI AfaI AluI
AlwI AlwNI AocI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspEI
BspMI BsrI BssKI BstF5I BstNI
BstXI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MroI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfcI SmaI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
TspRI TthHB8I Van91I XmaCI XmaI