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# NO CUT ENZYMES ( 47 enzymes) #
##################################
AatII AcyI AgeI Aor51HI AscI
BbeI BcgI BglI BsaHI BsiWI
BsmBI BspDI BssHII BssSI Bst1107I
BstZ17I ClaI CpoI EagI EclXI
Eco47III Eco52I EcoRI FseI Hin1I
KasI KspI MluI NarI NotI
NruI PacI PaeR7I PinAI PmeI
PvuI RsrII SacII SalI SbfI
SfiI SfoI SgrAI SnaBI Sse8387I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccII 3537 CGCG CG^CG
AccIII 8443 TCCGGA T^CCGGA
AclI 9896 AACGTT AA^CGTT
AhdI 9166 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 9166 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3235 TGCGCA TGC^GCA
AvrII 1299 CCTAGG C^CTAGG
BbrPI 504 CACGTG CAC^GTG
BlnI 1299 CCTAGG C^CTAGG
BlpI 523 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 523 GCTCAGC GC^T[ACGT]AGC
BsaWI 8443 TCCGGA [AT]^CCGG[AT]
BseAI 8443 TCCGGA T^CCGGA
BsiEI 8882 CGACCG CG[GA][CT]^CG
BspEI 8443 TCCGGA T^CCGGA
BsrBI 1974 CCGCTC CCGCTC(-3/-3)
BstBI 8428 TTCGAA TT^CGAA
BstEII 8450 GGTCACC G^GT[ACGT]ACC
BstPI 8450 GGTCACC G^GT[ACGT]ACC
BstUI 3537 CGCG CG^CG
CelII 523 GCTCAGC GC^T[ACGT]AGC
Eam1105I 9166 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1095 CCTCTGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 8450 GGTCACC G^GT[ACGT]ACC
EcoRV 6610 GATATC GAT^ATC
FspI 3235 TGCGCA TGC^GCA
MfeI 10073 CAATTG C^AATTG
MroI 8443 TCCGGA T^CCGGA
MunI 10073 CAATTG C^AATTG
MvnI 3537 CGCG CG^CG
NaeI 708 GCCGGC GCC^GGC
NgoMIV 708 GCCGGC G^CCGGC
NspV 8428 TTCGAA TT^CGAA
PmaCI 504 CACGTG CAC^GTG
PmlI 504 CACGTG CAC^GTG
PshAI 9212 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 9896 AACGTT AA^CGTT
SapI 2413 GAAGAGC GCTCTTC(1/4)
SfuI 8428 TTCGAA TT^CGAA
SmaI 1771 CCCGGG CCC^GGG
SpeI 2715 ACTAGT A^CTAGT
SwaI 10208 ATTTAAAT ATTT^AAAT
XmaCI 1771 CCCGGG C^CCGGG
XmaI 1771 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AseI 6271 ATTAAT AT^TAAT
7386 ATTAAT
AsnI 6271 ATTAAT AT^TAAT
7386 ATTAAT
AspI 2560 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4026 GACAAAGTC
BamHI 6198 GGATCC G^GATCC
7259 GGATCC
BsaAI 504 CACGTG [CT]AC^GT[GA]
8763 TACGTG
BsaBI 1619 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
9668 GATGCAGATC
BsrFI 708 GCCGGC [GA]^CCGG[CT]
3127 GCCGGT
Cfr10I 708 GCCGGC [GA]^CCGG[CT]
3127 GCCGGT
DraIII 1825 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
7854 CACTTGGTG
DrdI 1769 GACCCGGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5515 GACTGCTGTGTC
FauI 8833 CCCGC CCCGC(4/6)
1053 GCGGG
HaeII 8207 GGCGCT [GA]GCGC^[CT]
8620 GGCGCT
MamI 1619 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
9668 GATGCAGATC
PflFI 2560 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4026 GACAAAGTC
PshBI 6271 ATTAAT AT^TAAT
7386 ATTAAT
ScaI 5421 AGTACT AGT^ACT
6672 AGTACT
Tth111I 2560 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4026 GACAAAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 150 enzymes) #
#########################################
Acc65I AccI AciI AcsI AfaI
AflII AflIII AluI Alw44I AlwI
AlwNI AocI ApaI ApaLI ApoI
Asp700I Asp718I AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BciVI BclI BcnI BfaI
BfrI BglII BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspHI BspLU11I
BspMI BsrDI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
EcoT22I FbaI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HindIII HinfI HpaI HpaII
HphI ItaI KpnI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeI NdeII NheI NlaIII
NlaIV NsiI NspI PflMI PleI
PpuMI PstI PvuII RcaI RsaI
SacI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SmlI SphI SspBI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmnI