##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI Asp718I AspEI AspI AviII
BbrPI BcgI BciVI BglII BsaAI
BsaBI BsaWI BseAI BsgI BsiWI
BsmBI BspDI BspEI BsrBI BssHII
BstEII BstPI BstXI Bsu36I ClaI
CpoI DraIII Eam1105I Eco47III Eco81I
EcoO65I EcoRV FseI FspI HpaI
KpnI MamI MluI MroI NaeI
NgoMIV NotI NruI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SapI SbfI
ScaI SexAI SfiI SgrAI SnaBI
Sse8387I StuI SwaI Tth111I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AflII 3446 CTTAAG C^TTAAG
ApaI 149 GGGCCC GGGCC^C
BalI 3226 TGGCCA TGG^CCA
BclI 618 TGATCA T^GATCA
BfrI 3446 CTTAAG C^TTAAG
BsaI 1015 GGTCTC GGTCTC(1/5)
BsmI 3435 GAATGC GAATGC(1/-1)
Bsp1407I 3879 TGTACA T^GTACA
BspHI 2399 TCATGA T^CATGA
BsrFI 600 ACCGGC [GA]^CCGG[CT]
BsrGI 3879 TGTACA T^GTACA
BssSI 527 CTCGTG CACGAG(-5/-1)
Bst1107I 1920 GTATAC GTA^TAC
BstZ17I 1920 GTATAC GTA^TAC
Cfr10I 600 ACCGGC [GA]^CCGG[CT]
DrdI 1198 GACCCTGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 618 TGATCA T^GATCA
HindIII 3958 AAGCTT A^AGCTT
KspI 183 CCGCGG CCGC^GG
MluNI 3226 TGGCCA TGG^CCA
MscI 3226 TGGCCA TGG^CCA
NheI 4068 GCTAGC G^CTAGC
PshAI 4178 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2399 TCATGA T^CATGA
SacII 183 CCGCGG CCGC^GG
SalI 1207 GTCGAC G^TCGAC
SphI 402 GCATGC GCATG^C
SspBI 3879 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 44 enzymes) #
###################################
AccI 1207 GTCGAC GT^[AC][GT]AC
1920 GTATAC
AflIII 3137 ACATGT A^C[GA][CT]GT
4209 ACATGT
Asp700I 3078 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3957 GAAGCTTTTC
AvrII 397 CCTAGG C^CTAGG
1301 CCTAGG
BamHI 607 GGATCC G^GATCC
3772 GGATCC
BbeI 31 GGCGCC GGCGC^C
69 GGCGCC
BbsI 1893 GAAGAC GAAGAC(2/6)
4185 GTCTTC
BbvCI 433 CCTCAGC CCTCAGC(-5/-2)
281 GCTGAGG
BglI 34 GCCCCTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1115 GCCCTGGCGGC
BlnI 397 CCTAGG C^CTAGG
1301 CCTAGG
BlpI 706 GCTCAGC GC^T[ACGT]AGC
1437 GCTCAGC
Bpu1102I 706 GCTCAGC GC^T[ACGT]AGC
1437 GCTCAGC
BpuAI 1893 GAAGAC GAAGAC(2/6)
4185 GTCTTC
BsiEI 41 CGGCCG CG[GA][CT]^CG
189 CGGCCG
BspLU11I 3137 ACATGT A^CATGT
4209 ACATGT
BstBI 353 TTCGAA TT^CGAA
2650 TTCGAA
CelII 706 GCTCAGC GC^T[ACGT]AGC
1437 GCTCAGC
EagI 41 CGGCCG C^GGCCG
189 CGGCCG
EarI 815 GAAGAG CTCTTC(1/4)
3590 GAAGAG
EclXI 41 CGGCCG C^GGCCG
189 CGGCCG
Eco52I 41 CGGCCG C^GGCCG
189 CGGCCG
EcoNI 392 CCTGCCCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2861 CCTAAATGAGG
EcoT22I 1568 ATGCAT ATGCA^T
3237 ATGCAT
FauI 182 CCCGC CCCGC(4/6)
105 GCGGG
HgaI 1896 GACGC GACGC(5/10)
1205 GCGTC
KasI 31 GGCGCC G^GCGCC
69 GGCGCC
Ksp632I 815 GAAGAG CTCTTC(1/4)
3590 GAAGAG
MfeI 1493 CAATTG C^AATTG
3205 CAATTG
MunI 1493 CAATTG C^AATTG
3205 CAATTG
NarI 31 GGCGCC GG^CGCC
69 GGCGCC
NcoI 1416 CCATGG C^CATGG
1428 CCATGG
NsiI 1568 ATGCAT ATGCA^T
3237 ATGCAT
NspV 353 TTCGAA TT^CGAA
2650 TTCGAA
PflMI 332 CCAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
866 CCAGACTCTGG
SacI 8 GAGCTC GAGCT^C
4093 GAGCTC
SfoI 31 GGCGCC GGC^GCC
69 GGCGCC
SfuI 353 TTCGAA TT^CGAA
2650 TTCGAA
SmaI 65 CCCGGG CCC^GGG
146 CCCGGG
SpeI 4287 ACTAGT A^CTAGT
4324 ACTAGT
Van91I 332 CCAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
866 CCAGACTCTGG
XcmI 153 CCAGCTTCGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3197 CCATTTTTCAATTGG
XmaCI 65 CCCGGG C^CCGGG
146 CCCGGG
XmaI 65 CCCGGG C^CCGGG
146 CCCGGG
XmnI 3078 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3957 GAAGCTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AseI
AsnI AspHI AvaI AvaII BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaHI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EcoO109I
EcoRI EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeI NdeII NlaIII
NlaIV NspI PleI PpuMI PshBI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII