##################################
# NO CUT ENZYMES ( 43 enzymes) #
##################################
AatII AcyI AgeI AscI AspI
BbeI BsaHI BsiWI BsrBI BssHII
BstBI DrdI FseI Hin1I HpaI
KasI KspI MluI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PflFI PinAI PmeI PvuI
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SmaI Sse8387I
Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AclI 11031 AACGTT AA^CGTT
AhdI 10625 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2750 AGCGCT AGC^GCT
ApaI 1571 GGGCCC GGGCC^C
AspEI 10625 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 9260 CCTAGG C^CTAGG
BciVI 44 GGATAC GTATCC(6/5)
BlnI 9260 CCTAGG C^CTAGG
BlpI 9855 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 9855 GCTCAGC GC^T[ACGT]AGC
BsiEI 2084 CGGCCG CG[GA][CT]^CG
BsmBI 4581 CGTCTC CGTCTC(1/5)
BsmFI 10623 GGGAC GGGAC(10/14)
CelII 9855 GCTCAGC GC^T[ACGT]AGC
CpoI 4196 CGGTCCG CG^G[AT]CCG
EagI 2084 CGGCCG C^GGCCG
Eam1105I 10625 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2084 CGGCCG C^GGCCG
Eco47III 2750 AGCGCT AGC^GCT
Eco52I 2084 CGGCCG C^GGCCG
MfeI 7728 CAATTG C^AATTG
MunI 7728 CAATTG C^AATTG
PaeR7I 127 CTCGAG C^TCGAG
PshAI 9910 GACTTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 11031 AACGTT AA^CGTT
RsrII 4196 CGGTCCG CG^G[AT]CCG
SexAI 10074 ACCAGGT A^CC[AT]GGT
SnaBI 303 TACGTA TAC^GTA
SpeI 9445 ACTAGT A^CTAGT
SwaI 11358 ATTTAAAT ATTT^AAAT
XhoI 127 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 3444 GGTACC G^GTACC
5719 GGTACC
AccIII 2315 TCCGGA T^CCGGA
2987 TCCGGA
Asp718I 3444 GGTACC G^GTACC
5719 GGTACC
BcgI 6715 GCACGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
6750 GCAATGAGATCG
BclI 4497 TGATCA T^GATCA
4973 TGATCA
BglI 562 GCCAGTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
6621 GCCAACATGGC
BsaBI 403 GATATGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1019 GATTTACATC
BsaWI 2315 TCCGGA [AT]^CCGG[AT]
2987 TCCGGA
BseAI 2315 TCCGGA T^CCGGA
2987 TCCGGA
BspDI 3044 ATCGAT AT^CGAT
6723 ATCGAT
BspEI 2315 TCCGGA T^CCGGA
2987 TCCGGA
Bst1107I 5277 GTATAC GTA^TAC
8332 GTATAC
BstEII 3711 GGTGACC G^GT[ACGT]ACC
9254 GGTAACC
BstPI 3711 GGTGACC G^GT[ACGT]ACC
9254 GGTAACC
BstXI 6559 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
10152 CCAAGTCACTGG
BstZ17I 5277 GTATAC GTA^TAC
8332 GTATAC
ClaI 3044 ATCGAT AT^CGAT
6723 ATCGAT
EcoNI 4041 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5037 CCTGCGGAAGG
EcoO65I 3711 GGTGACC G^GT[ACGT]ACC
9254 GGTAACC
EcoRI 8715 GAATTC G^AATTC
11525 GAATTC
FbaI 4497 TGATCA T^GATCA
4973 TGATCA
HincII 4312 GTCAAC GT[CT]^[GA]AC
5093 GTCAAC
HindII 4312 GTCAAC GT[CT]^[GA]AC
5093 GTCAAC
HindIII 437 AAGCTT A^AGCTT
1640 AAGCTT
KpnI 3444 GGTACC GGTAC^C
5719 GGTACC
MamI 403 GATATGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1019 GATTTACATC
MroI 2315 TCCGGA T^CCGGA
2987 TCCGGA
NdeI 9659 CATATG CA^TATG
11022 CATATG
PflMI 35 CCAGGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
11241 CCAACAAATGG
ScaI 2471 AGTACT AGT^ACT
5587 AGTACT
StuI 932 AGGCCT AGG^CCT
10099 AGGCCT
Van91I 35 CCAGGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
11241 CCAACAAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 152 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflII AflIII AluI Alw44I AlwI
AlwNI AocI ApaLI ApoI AseI
AsnI Asp700I AspHI AvaI AvaII
AviII BalI BamHI BanI BanII
BbrPI BbsI BbvCI BbvI BcnI
BfaI BfrI BglII BmyI BpmI
BpuAI BsaAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmI BsoBI Bsp1286I Bsp1407I BspHI
BspLU11I BspMI BsrDI BsrFI BsrGI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoRV
EcoT14I EcoT22I FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NsiI NspI
PleI PmaCI PmlI PpuMI PshBI
PstI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XcmI
XhoII XmnI