################################## # NO CUT ENZYMES ( 47 enzymes) # ################################## AatII AflII AhdI AscI AspEI AspI BbrPI BcgI BfrI BsiWI BsmBI Bsp1407I BspDI BsrGI BssHII BstBI ClaI CpoI Eam1105I EcoRV FseI KspI MluI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PvuI RsrII SacII SalI ScaI SexAI SfuI SgrAI SnaBI SspBI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccIII 4051 TCCGGA T^CCGGA AclI 9785 AACGTT AA^CGTT AgeI 8594 ACCGGT A^CCGGT Aor51HI 450 AGCGCT AGC^GCT AseI 9578 ATTAAT AT^TAAT AsnI 9578 ATTAAT AT^TAAT AvrII 4199 CCTAGG C^CTAGG BlnI 4199 CCTAGG C^CTAGG BsaBI 2027 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 4051 TCCGGA T^CCGGA BsiEI 5379 CGGCCG CG[GA][CT]^CG BspEI 4051 TCCGGA T^CCGGA BspHI 9454 TCATGA T^CATGA BssSI 5671 CACGAG CACGAG(-5/-1) Bst1107I 9483 GTATAC GTA^TAC BstZ17I 9483 GTATAC GTA^TAC DrdI 6714 GACCACACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 5379 CGGCCG C^GGCCG EclXI 5379 CGGCCG C^GGCCG Eco47III 450 AGCGCT AGC^GCT Eco52I 5379 CGGCCG C^GGCCG EcoT22I 4360 ATGCAT ATGCA^T HpaI 9863 GTTAAC GTT^AAC MamI 2027 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 6630 CAATTG C^AATTG MroI 4051 TCCGGA T^CCGGA MunI 6630 CAATTG C^AATTG NsiI 4360 ATGCAT ATGCA^T PinAI 8594 ACCGGT A^CCGGT PshAI 1543 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 9578 ATTAAT AT^TAAT Psp1406I 9785 AACGTT AA^CGTT RcaI 9454 TCATGA T^CATGA SapI 4389 GCTCTTC GCTCTTC(1/4) SfiI 5400 GGCCTGTGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SpeI 8620 ACTAGT A^CTAGT SphI 2861 GCATGC GCATG^C ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### Acc65I 2183 GGTACC G^GTACC 6239 GGTACC Alw44I 548 GTGCAC G^TGCAC 8083 GTGCAC ApaLI 548 GTGCAC G^TGCAC 8083 GTGCAC Asp718I 2183 GGTACC G^GTACC 6239 GGTACC BamHI 3045 GGATCC G^GATCC 7606 GGATCC BbeI 736 GGCGCC GGCGC^C 5433 GGCGCC BciVI 1325 GGATAC GTATCC(6/5) 9897 GGATAC BsaAI 1058 TACGTG [CT]AC^GT[GA] 2784 CACGTA BsaWI 4051 TCCGGA [AT]^CCGG[AT] 8594 ACCGGT BspLU11I 1709 ACATGT A^CATGT 7280 ACATGT BstAPI 1087 GCACATCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 7202 GCACCAGATGC BstEII 2679 GGTCACC G^GT[ACGT]ACC 5822 GGTGACC BstPI 2679 GGTCACC G^GT[ACGT]ACC 5822 GGTGACC EcoNI 5391 CCTTGTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 6640 CCTAATGAAGG EcoO65I 2679 GGTCACC G^GT[ACGT]ACC 5822 GGTGACC KasI 736 GGCGCC G^GCGCC 5433 GGCGCC KpnI 2183 GGTACC GGTAC^C 6239 GGTACC NaeI 733 GCCGGC GCC^GGC 5055 GCCGGC NarI 736 GGCGCC GG^CGCC 5433 GGCGCC NgoMIV 733 GCCGGC G^CCGGC 5055 GCCGGC SbfI 1738 CCTGCAGG CCTGCA^GG 7536 CCTGCAGG SfoI 736 GGCGCC GGC^GCC 5433 GGCGCC Sse8387I 1738 CCTGCAGG CCTGCA^GG 7536 CCTGCAGG SspI 8691 AATATT AAT^ATT 8870 AATATT XbaI 3769 TCTAGA T^CTAGA 8291 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 149 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI AocI ApaI ApoI Asp700I AspHI AvaI AvaII AviII BalI BanI BanII BbsI BbvCI BbvI BclI BcnI BfaI BglI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI Eco81I EcoO109I EcoRI EcoRII EcoT14I FauI FbaI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI XmnI