##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
AatII AflII AhdI AscI AspEI
AspI BbrPI BcgI BfrI BsiWI
BsmBI Bsp1407I BspDI BsrGI BssHII
BstBI ClaI CpoI Eam1105I EcoRV
FseI KspI MluI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PvuI
RsrII SacII SalI ScaI SexAI
SfuI SgrAI SnaBI SspBI SwaI
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccIII 4051 TCCGGA T^CCGGA
AclI 9785 AACGTT AA^CGTT
AgeI 8594 ACCGGT A^CCGGT
Aor51HI 450 AGCGCT AGC^GCT
AseI 9578 ATTAAT AT^TAAT
AsnI 9578 ATTAAT AT^TAAT
AvrII 4199 CCTAGG C^CTAGG
BlnI 4199 CCTAGG C^CTAGG
BsaBI 2027 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 4051 TCCGGA T^CCGGA
BsiEI 5379 CGGCCG CG[GA][CT]^CG
BspEI 4051 TCCGGA T^CCGGA
BspHI 9454 TCATGA T^CATGA
BssSI 5671 CACGAG CACGAG(-5/-1)
Bst1107I 9483 GTATAC GTA^TAC
BstZ17I 9483 GTATAC GTA^TAC
DrdI 6714 GACCACACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 5379 CGGCCG C^GGCCG
EclXI 5379 CGGCCG C^GGCCG
Eco47III 450 AGCGCT AGC^GCT
Eco52I 5379 CGGCCG C^GGCCG
EcoT22I 4360 ATGCAT ATGCA^T
HpaI 9863 GTTAAC GTT^AAC
MamI 2027 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 6630 CAATTG C^AATTG
MroI 4051 TCCGGA T^CCGGA
MunI 6630 CAATTG C^AATTG
NsiI 4360 ATGCAT ATGCA^T
PinAI 8594 ACCGGT A^CCGGT
PshAI 1543 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 9578 ATTAAT AT^TAAT
Psp1406I 9785 AACGTT AA^CGTT
RcaI 9454 TCATGA T^CATGA
SapI 4389 GCTCTTC GCTCTTC(1/4)
SfiI 5400 GGCCTGTGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 8620 ACTAGT A^CTAGT
SphI 2861 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Acc65I 2183 GGTACC G^GTACC
6239 GGTACC
Alw44I 548 GTGCAC G^TGCAC
8083 GTGCAC
ApaLI 548 GTGCAC G^TGCAC
8083 GTGCAC
Asp718I 2183 GGTACC G^GTACC
6239 GGTACC
BamHI 3045 GGATCC G^GATCC
7606 GGATCC
BbeI 736 GGCGCC GGCGC^C
5433 GGCGCC
BciVI 1325 GGATAC GTATCC(6/5)
9897 GGATAC
BsaAI 1058 TACGTG [CT]AC^GT[GA]
2784 CACGTA
BsaWI 4051 TCCGGA [AT]^CCGG[AT]
8594 ACCGGT
BspLU11I 1709 ACATGT A^CATGT
7280 ACATGT
BstAPI 1087 GCACATCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
7202 GCACCAGATGC
BstEII 2679 GGTCACC G^GT[ACGT]ACC
5822 GGTGACC
BstPI 2679 GGTCACC G^GT[ACGT]ACC
5822 GGTGACC
EcoNI 5391 CCTTGTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
6640 CCTAATGAAGG
EcoO65I 2679 GGTCACC G^GT[ACGT]ACC
5822 GGTGACC
KasI 736 GGCGCC G^GCGCC
5433 GGCGCC
KpnI 2183 GGTACC GGTAC^C
6239 GGTACC
NaeI 733 GCCGGC GCC^GGC
5055 GCCGGC
NarI 736 GGCGCC GG^CGCC
5433 GGCGCC
NgoMIV 733 GCCGGC G^CCGGC
5055 GCCGGC
SbfI 1738 CCTGCAGG CCTGCA^GG
7536 CCTGCAGG
SfoI 736 GGCGCC GGC^GCC
5433 GGCGCC
Sse8387I 1738 CCTGCAGG CCTGCA^GG
7536 CCTGCAGG
SspI 8691 AATATT AAT^ATT
8870 AATATT
XbaI 3769 TCTAGA T^CTAGA
8291 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 149 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
AocI ApaI ApoI Asp700I AspHI
AvaI AvaII AviII BalI BanI
BanII BbsI BbvCI BbvI BclI
BcnI BfaI BglI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EaeI EarI Eco81I EcoO109I EcoRI
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI XmnI