##################################
# NO CUT ENZYMES ( 44 enzymes) #
##################################
AclI AflII AhdI Aor51HI AscI
AspEI AspI AviII BclI BfrI
BsiEI BssHII Bst1107I BstZ17I CpoI
EagI Eam1105I EclXI Eco47III Eco52I
FbaI FseI FspI HpaI NotI
NruI PacI PaeR7I PflFI PmeI
Psp1406I PvuI RsrII SalI SgrAI
SmaI SnaBI SpeI SwaI Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 3051 GACGTC GACGT^C
Acc65I 6988 GGTACC G^GTACC
AgeI 9267 ACCGGT A^CCGGT
AocI 5555 CCTTAGG CC^T[ACGT]AGG
ApaI 3325 GGGCCC GGGCC^C
AseI 8819 ATTAAT AT^TAAT
AsnI 8819 ATTAAT AT^TAAT
Asp718I 6988 GGTACC G^GTACC
BamHI 7523 GGATCC G^GATCC
BbeI 39 GGCGCC GGCGC^C
BbrPI 6733 CACGTG CAC^GTG
BciVI 8683 GGATAC GTATCC(6/5)
BglI 7153 GCCAACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 6733 CACGTG [CT]AC^GT[GA]
BsiWI 4162 CGTACG C^GTACG
BspDI 2908 ATCGAT AT^CGAT
BssSI 1633 CTCGTG CACGAG(-5/-1)
BstAPI 3245 GCAGCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 5555 CCTTAGG CC^T[ACGT]AGG
ClaI 2908 ATCGAT AT^CGAT
DrdI 6788 GACAGTGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 5555 CCTTAGG CC^T[ACGT]AGG
KasI 39 GGCGCC G^GCGCC
KpnI 6988 GGTACC GGTAC^C
KspI 293 CCGCGG CCGC^GG
MluI 1656 ACGCGT A^CGCGT
NaeI 2155 GCCGGC GCC^GGC
NarI 39 GGCGCC GG^CGCC
NgoMIV 2155 GCCGGC G^CCGGC
PinAI 9267 ACCGGT A^CCGGT
PmaCI 6733 CACGTG CAC^GTG
PmlI 6733 CACGTG CAC^GTG
PshAI 7440 GACATTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 8819 ATTAAT AT^TAAT
SacII 293 CCGCGG CCGC^GG
SapI 8270 GCTCTTC GCTCTTC(1/4)
SbfI 3015 CCTGCAGG CCTGCA^GG
SfiI 7152 GGCCAACTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 39 GGCGCC GGC^GCC
Sse8387I 3015 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccIII 2827 TCCGGA T^CCGGA
3735 TCCGGA
AcyI 39 GGCGCC G[GA]^CG[CT]C
3051 GACGTC
Alw44I 766 GTGCAC G^TGCAC
4550 GTGCAC
ApaLI 766 GTGCAC G^TGCAC
4550 GTGCAC
BbvCI 3431 CCTCAGC CCTCAGC(-5/-2)
7188 CCTCAGC
BsaHI 39 GGCGCC G[GA]^CG[CT]C
3051 GACGTC
BseAI 2827 TCCGGA T^CCGGA
3735 TCCGGA
BspEI 2827 TCCGGA T^CCGGA
3735 TCCGGA
BspHI 4124 TCATGA T^CATGA
7681 TCATGA
BsrFI 2155 GCCGGC [GA]^CCGG[CT]
9267 ACCGGT
BstBI 1538 TTCGAA TT^CGAA
4052 TTCGAA
BstEII 2707 GGTGACC G^GT[ACGT]ACC
5513 GGTCACC
BstPI 2707 GGTGACC G^GT[ACGT]ACC
5513 GGTCACC
Cfr10I 2155 GCCGGC [GA]^CCGG[CT]
9267 ACCGGT
DraIII 784 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
4614 CACTTGGTG
EcoO65I 2707 GGTGACC G^GT[ACGT]ACC
5513 GGTCACC
HaeII 39 GGCGCC [GA]GCGC^[CT]
2284 GGCGCT
Hin1I 39 GGCGCC G[GA]^CG[CT]C
3051 GACGTC
MroI 2827 TCCGGA T^CCGGA
3735 TCCGGA
NdeI 5142 CATATG CA^TATG
10893 CATATG
NheI 125 GCTAGC G^CTAGC
5085 GCTAGC
NspV 1538 TTCGAA TT^CGAA
4052 TTCGAA
RcaI 4124 TCATGA T^CATGA
7681 TCATGA
ScaI 946 AGTACT AGT^ACT
7259 AGTACT
SexAI 2443 ACCTGGT A^CC[AT]GGT
5416 ACCTGGT
SfuI 1538 TTCGAA TT^CGAA
4052 TTCGAA
SphI 983 GCATGC GCATG^C
8219 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 147 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp700I AspHI AvaI AvaII AvrII
BalI BanI BanII BbsI BbvI
BcgI BcnI BfaI BglII BlnI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaBI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspLU11I BspMI BsrBI BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRI EcoRII EcoRV EcoT14I
EcoT22I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MfeI MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MunI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NsiI NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmnI