##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII AccI AccIII AclI Alw44I
ApaLI AscI AspI AvrII BbrPI
BbvCI BcgI BciVI BglI BlnI
BseAI BsgI BsiWI Bsp1407I BspDI
BspEI BsrBI BsrGI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
ClaI CpoI DraIII DrdI EcoO65I
EcoRI EcoRV EcoT22I FseI MfeI
MluI MroI MunI NheI NotI
NruI NsiI NspV PflFI PflMI
PmaCI PmeI PmlI PshAI Psp1406I
PstI PvuI RsrII SalI SbfI
ScaI SfiI SfuI SgrAI SpeI
Sse8387I SspBI SwaI Tth111I Van91I
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AflII 939 CTTAAG C^TTAAG
AgeI 445 ACCGGT A^CCGGT
AhdI 3327 GACCAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2366 CCTGAGG CC^T[ACGT]AGG
ApaI 183 GGGCCC GGGCC^C
AspEI 3327 GACCAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1804 GGATCC G^GATCC
BbsI 2361 GAAGAC GAAGAC(2/6)
BfrI 939 CTTAAG C^TTAAG
BglII 4632 AGATCT A^GATCT
BlpI 38 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 38 GCTGAGC GC^T[ACGT]AGC
BpuAI 2361 GAAGAC GAAGAC(2/6)
BsaAI 2830 TACGTA [CT]AC^GT[GA]
BsaBI 2324 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 119 CGGCCG CG[GA][CT]^CG
BspHI 1067 TCATGA T^CATGA
Bsu36I 2366 CCTGAGG CC^T[ACGT]AGG
CelII 38 GCTGAGC GC^T[ACGT]AGC
EagI 119 CGGCCG C^GGCCG
Eam1105I 3327 GACCAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 119 CGGCCG C^GGCCG
Eco52I 119 CGGCCG C^GGCCG
Eco81I 2366 CCTGAGG CC^T[ACGT]AGG
HgaI 189 GCGTC GACGC(5/10)
HpaI 435 GTTAAC GTT^AAC
MamI 2324 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 2009 CACTCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 121 GCCGGC GCC^GGC
NcoI 271 CCATGG C^CATGG
NdeI 4927 CATATG CA^TATG
NgoMIV 121 GCCGGC G^CCGGC
PacI 3986 TTAATTAA TTAAT^TAA
PaeR7I 1141 CTCGAG C^TCGAG
PinAI 445 ACCGGT A^CCGGT
RcaI 1067 TCATGA T^CATGA
SmaI 175 CCCGGG CCC^GGG
SnaBI 2830 TACGTA TAC^GTA
SphI 2786 GCATGC GCATG^C
XhoI 1141 CTCGAG C^TCGAG
XmaCI 175 CCCGGG C^CCGGG
XmaI 175 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 2122 GGTACC G^GTACC
2509 GGTACC
AcyI 236 GGCGCC G[GA]^CG[CT]C
3686 GGCGCC
AlwNI 1678 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
2791 CAGTTGCTG
Aor51HI 382 AGCGCT AGC^GCT
2602 AGCGCT
Asp700I 2846 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4411 GAAGTCATTC
Asp718I 2122 GGTACC G^GTACC
2509 GGTACC
AviII 59 TGCGCA TGC^GCA
2495 TGCGCA
BbeI 236 GGCGCC GGCGC^C
3686 GGCGCC
BclI 1217 TGATCA T^GATCA
3473 TGATCA
BsaHI 236 GGCGCC G[GA]^CG[CT]C
3686 GGCGCC
BsaWI 204 ACCGGA [AT]^CCGG[AT]
445 ACCGGT
BspMI 961 GCAGGT ACCTGC(4/8)
1345 GCAGGT
BssHII 103 GCGCGC G^CGCGC
110 GCGCGC
BstXI 3329 CCAAAAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3614 CCAGAAAAATGG
DraII 2266 AGGTCCT [GA]G^G[ACGT]CC[CT]
2646 GGGACCC
Eco47III 382 AGCGCT AGC^GCT
2602 AGCGCT
EcoO109I 2266 AGGTCCT [GA]G^G[ACGT]CC[CT]
2646 GGGACCC
FbaI 1217 TGATCA T^GATCA
3473 TGATCA
FspI 59 TGCGCA TGC^GCA
2495 TGCGCA
Hin1I 236 GGCGCC G[GA]^CG[CT]C
3686 GGCGCC
HincII 435 GTTAAC GT[CT]^[GA]AC
1851 GTTGAC
HindII 435 GTTAAC GT[CT]^[GA]AC
1851 GTTGAC
KasI 236 GGCGCC G^GCGCC
3686 GGCGCC
KpnI 2122 GGTACC GGTAC^C
2509 GGTACC
KspI 77 CCGCGG CCGC^GG
154 CCGCGG
NarI 236 GGCGCC GG^CGCC
3686 GGCGCC
PpuMI 2266 AGGTCCT [GA]G^G[AT]CC[CT]
2646 GGGACCC
SacI 924 GAGCTC GAGCT^C
3389 GAGCTC
SacII 77 CCGCGG CCGC^GG
154 CCGCGG
SapI 921 GAAGAGC GCTCTTC(1/4)
1536 GAAGAGC
SexAI 1834 ACCAGGT A^CC[AT]GGT
4297 ACCAGGT
SfoI 236 GGCGCC GGC^GCC
3686 GGCGCC
StuI 1968 AGGCCT AGG^CCT
2179 AGGCCT
XmnI 2846 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4411 GAAGTCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI AseI AsnI
AspHI AvaI AvaII BalI BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I BspLU11I BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DsaI
EaeI EarI EcoNI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PshBI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI
XcmI XhoII