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# NO CUT ENZYMES ( 24 enzymes) #
##################################
AseI AsnI AspI BsiWI BspLU11I
Bst1107I BstBI BstZ17I EcoRI FseI
MluI NotI NruI NspV PacI
PflFI PshBI PvuI SacI SalI
SexAI SfuI Tth111I XbaI
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# ONE CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 2061 GGTACC G^GTACC
AclI 11639 AACGTT AA^CGTT
AhdI 4106 GACGTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3470 AGCGCT AGC^GCT
AscI 271 GGCGCGCC GG^CGCGCC
Asp718I 2061 GGTACC G^GTACC
AspEI 4106 GACGTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BsaBI 5864 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
CpoI 6569 CGGACCG CG^G[AT]CCG
DraIII 3704 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 6575 GACTGACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 4106 GACGTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3470 AGCGCT AGC^GCT
HpaI 6422 GTTAAC GTT^AAC
KpnI 2061 GGTACC GGTAC^C
MamI 5864 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3697 CAATTG C^AATTG
MunI 3697 CAATTG C^AATTG
NdeI 8812 CATATG CA^TATG
NheI 10435 GCTAGC G^CTAGC
PaeR7I 806 CTCGAG C^TCGAG
PflMI 2166 CCAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmeI 8475 GTTTAAAC GTTT^AAAC
Psp1406I 11639 AACGTT AA^CGTT
RsrII 6569 CGGACCG CG^G[AT]CCG
SbfI 7792 CCTGCAGG CCTGCA^GG
SfiI 9029 GGCCGACCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 11205 CACCGGTG C[GA]^CCGG[CT]G
SnaBI 10962 TACGTA TAC^GTA
SpeI 10183 ACTAGT A^CTAGT
Sse8387I 7792 CCTGCAGG CCTGCA^GG
SspI 6288 AATATT AAT^ATT
SwaI 7137 ATTTAAAT ATTT^AAAT
Van91I 2166 CCAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 806 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
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AatII 2000 GACGTC GACGT^C
4106 GACGTC
AccI 8023 GTAGAC GT^[AC][GT]AC
11287 GTCTAC
AccIII 542 TCCGGA T^CCGGA
5268 TCCGGA
AviII 4260 TGCGCA TGC^GCA
4666 TGCGCA
BlpI 9554 GCTAAGC GC^T[ACGT]AGC
9952 GCTCAGC
Bpu1102I 9554 GCTAAGC GC^T[ACGT]AGC
9952 GCTCAGC
BseAI 542 TCCGGA T^CCGGA
5268 TCCGGA
BsiEI 127 CGGCCG CG[GA][CT]^CG
2802 CGGCCG
BsmBI 8117 CGTCTC CGTCTC(1/5)
11216 GAGACG
BspDI 2930 ATCGAT AT^CGAT
9315 ATCGAT
BspEI 542 TCCGGA T^CCGGA
5268 TCCGGA
BsrBI 11 CCGCTC CCGCTC(-3/-3)
4592 GAGCGG
BssHII 272 GCGCGC G^CGCGC
1248 GCGCGC
BstAPI 1482 GCATGTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
11359 GCAAATAATGC
BstEII 7332 GGTAACC G^GT[ACGT]ACC
8377 GGTGACC
BstPI 7332 GGTAACC G^GT[ACGT]ACC
8377 GGTGACC
CelII 9554 GCTAAGC GC^T[ACGT]AGC
9952 GCTCAGC
ClaI 2930 ATCGAT AT^CGAT
9315 ATCGAT
EagI 127 CGGCCG C^GGCCG
2802 CGGCCG
EclXI 127 CGGCCG C^GGCCG
2802 CGGCCG
Eco52I 127 CGGCCG C^GGCCG
2802 CGGCCG
EcoO65I 7332 GGTAACC G^GT[ACGT]ACC
8377 GGTGACC
EcoRV 2933 GATATC GAT^ATC
4001 GATATC
EcoT22I 9482 ATGCAT ATGCA^T
11366 ATGCAT
FspI 4260 TGCGCA TGC^GCA
4666 TGCGCA
MroI 542 TCCGGA T^CCGGA
5268 TCCGGA
NsiI 9482 ATGCAT ATGCA^T
11366 ATGCAT
PshAI 1067 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4229 GACAACTGTC
SapI 2834 GAAGAGC GCTCTTC(1/4)
9109 GAAGAGC
SphI 1437 GCATGC GCATG^C
3645 GCATGC
StuI 3056 AGGCCT AGG^CCT
4292 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 168 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflII AflIII AgeI AluI Alw44I
AlwI AlwNI AocI ApaI ApaLI
ApoI Asp700I AspHI AvaI AvaII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BbvI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BglII BlnI BmyI
BpmI BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspHI BspMI
BsrDI BsrFI BsrGI BsrI BssKI
BssSI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI KasI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PinAI
PleI PmaCI PmlI PpuMI PstI
PvuII RcaI RsaI SacII Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SfoI SmaI SmlI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmaCI XmaI XmnI