##################################
# NO CUT ENZYMES ( 26 enzymes) #
##################################
AflII AgeI AscI AseI AsnI
AvrII BfrI BlnI BsiWI BspDI
Bst1107I BstZ17I ClaI CpoI DrdI
EcoRV FseI NotI NruI PacI
PinAI PmeI PshBI RsrII SnaBI
SpeI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AatII 471 GACGTC GACGT^C
Acc65I 526 GGTACC G^GTACC
AclI 1079 AACGTT AA^CGTT
Asp718I 526 GGTACC G^GTACC
BbeI 2214 GGCGCC GGCGC^C
BbvCI 317 CCTCAGC CCTCAGC(-5/-2)
BstBI 10668 TTCGAA TT^CGAA
BstEII 9021 GGTAACC G^GT[ACGT]ACC
BstPI 9021 GGTAACC G^GT[ACGT]ACC
EagI 10567 CGGCCG C^GGCCG
EclXI 10567 CGGCCG C^GGCCG
Eco52I 10567 CGGCCG C^GGCCG
EcoO65I 9021 GGTAACC G^GT[ACGT]ACC
HpaI 6426 GTTAAC GTT^AAC
KasI 2214 GGCGCC G^GCGCC
KpnI 526 GGTACC GGTAC^C
MluI 8906 ACGCGT A^CGCGT
NarI 2214 GGCGCC GG^CGCC
NheI 11542 GCTAGC G^CTAGC
NspV 10668 TTCGAA TT^CGAA
PaeR7I 4912 CTCGAG C^TCGAG
Psp1406I 1079 AACGTT AA^CGTT
PvuI 4918 CGATCG CGAT^CG
SalI 8787 GTCGAC G^TCGAC
SfiI 10771 GGCCGCCACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2214 GGCGCC GGC^GCC
SfuI 10668 TTCGAA TT^CGAA
SgrAI 5535 CGCCGGTG C[GA]^CCGG[CT]G
SspI 11636 AATATT AAT^ATT
SwaI 10246 ATTTAAAT ATTT^AAAT
XbaI 11454 TCTAGA T^CTAGA
XhoI 4912 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Alw44I 291 GTGCAC G^TGCAC
7950 GTGCAC
ApaI 1904 GGGCCC GGGCC^C
9051 GGGCCC
ApaLI 291 GTGCAC G^TGCAC
7950 GTGCAC
AviII 2793 TGCGCA TGC^GCA
3556 TGCGCA
BciVI 7830 GGATAC GTATCC(6/5)
8912 GTATCC
BsmBI 9347 CGTCTC CGTCTC(1/5)
469 GAGACG
BssHII 1748 GCGCGC G^CGCGC
7174 GCGCGC
EcoRI 3326 GAATTC G^AATTC
10233 GAATTC
EcoT22I 2925 ATGCAT ATGCA^T
8236 ATGCAT
FspI 2793 TGCGCA TGC^GCA
3556 TGCGCA
KspI 7826 CCGCGG CCGC^GG
10705 CCGCGG
MfeI 517 CAATTG C^AATTG
2428 CAATTG
MunI 517 CAATTG C^AATTG
2428 CAATTG
NaeI 4958 GCCGGC GCC^GGC
8692 GCCGGC
NdeI 2446 CATATG CA^TATG
3420 CATATG
NgoMIV 4958 GCCGGC G^CCGGC
8692 GCCGGC
NsiI 2925 ATGCAT ATGCA^T
8236 ATGCAT
SacII 7826 CCGCGG CCGC^GG
10705 CCGCGG
SbfI 2999 CCTGCAGG CCTGCA^GG
4868 CCTGCAGG
Sse8387I 2999 CCTGCAGG CCTGCA^GG
4868 CCTGCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 180 enzymes) #
#########################################
AccI AccII AccIII AciI AcsI
AcyI AfaI AflIII AhdI AluI
AlwI AlwNI AocI Aor51HI ApoI
Asp700I AspEI AspHI AspI AvaI
AvaII BalI BamHI BanI BanII
BbrPI BbsI BbvI BcgI BclI
BcnI BfaI BglI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI Eam1105I EarI
Eco47III Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MroI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflFI
PflMI PleI PmaCI PmlI PpuMI
PshAI PstI PvuII RcaI RsaI
SacI SapI Sau3AI Sau96I ScaI
ScrFI SexAI SfaNI SfcI SmaI
SmlI SphI SspBI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII XmaCI XmaI XmnI