##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII Acc65I AccIII AflII AhdI
Aor51HI Asp718I AspEI AviII BbeI
BfrI BsaBI BseAI BsiEI BsiWI
BspDI BspEI BstBI ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV FseI FspI KasI KpnI
MamI MluI MroI NaeI NarI
NgoMIV NheI NotI NspV PacI
PshAI PvuI RsrII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SnaBI Sse8387I SwaI XbaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AclI 7136 AACGTT AA^CGTT
AgeI 1520 ACCGGT A^CCGGT
AscI 8041 GGCGCGCC GG^CGCGCC
BamHI 4833 GGATCC G^GATCC
BanI 6961 GGCACC G^G[CT][GA]CC
BbrPI 4726 CACGTG CAC^GTG
BclI 6202 TGATCA T^GATCA
BglI 6814 GCCATGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 9134 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 9134 GCTTAGC GC^T[ACGT]AGC
BssSI 3543 CTCGTG CACGAG(-5/-1)
Bst1107I 6635 GTATAC GTA^TAC
BstEII 4177 GGTAACC G^GT[ACGT]ACC
BstPI 4177 GGTAACC G^GT[ACGT]ACC
BstZ17I 6635 GTATAC GTA^TAC
CelII 9134 GCTTAGC GC^T[ACGT]AGC
DrdI 6623 GACATAAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 4177 GGTAACC G^GT[ACGT]ACC
FbaI 6202 TGATCA T^GATCA
MfeI 10100 CAATTG C^AATTG
MunI 10100 CAATTG C^AATTG
NruI 8391 TCGCGA TCG^CGA
PaeR7I 2368 CTCGAG C^TCGAG
PflMI 4794 CCAGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1520 ACCGGT A^CCGGT
PmaCI 4726 CACGTG CAC^GTG
PmeI 2498 GTTTAAAC GTTT^AAAC
PmlI 4726 CACGTG CAC^GTG
PpuMI 10495 GGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 7136 AACGTT AA^CGTT
SacI 10367 GAGCTC GAGCT^C
ScaI 5547 AGTACT AGT^ACT
SpeI 10113 ACTAGT A^CTAGT
Van91I 4794 CCAGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2368 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Alw44I 3190 GTGCAC G^TGCAC
7827 GTGCAC
AocI 5148 CCTCAGG CC^T[ACGT]AGG
8021 CCTCAGG
ApaLI 3190 GTGCAC G^TGCAC
7827 GTGCAC
AseI 3781 ATTAAT AT^TAAT
6602 ATTAAT
AsnI 3781 ATTAAT AT^TAAT
6602 ATTAAT
AspI 3344 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
8352 GACGGAGTC
BsaWI 1520 ACCGGT [AT]^CCGG[AT]
4314 ACCGGA
BspHI 1764 TCATGA T^CATGA
8906 TCATGA
BsrBI 2393 CCGCTC CCGCTC(-3/-3)
3392 CCGCTC
BsrFI 1520 ACCGGT [GA]^CCGG[CT]
9781 GCCGGT
BstAPI 1444 GCAACTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4981 GCAGCCTTTGC
Bsu36I 5148 CCTCAGG CC^T[ACGT]AGG
8021 CCTCAGG
Cfr10I 1520 ACCGGT [GA]^CCGG[CT]
9781 GCCGGT
DraIII 8815 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
8979 CACCATGTG
Eco81I 5148 CCTCAGG CC^T[ACGT]AGG
8021 CCTCAGG
HaeII 7701 GGCGCT [GA]GCGC^[CT]
9778 AGCGCC
KspI 7715 CCGCGG CCGC^GG
8079 CCGCGG
NcoI 6815 CCATGG C^CATGG
10663 CCATGG
PflFI 3344 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
8352 GACGGAGTC
PshBI 3781 ATTAAT AT^TAAT
6602 ATTAAT
RcaI 1764 TCATGA T^CATGA
8906 TCATGA
SacII 7715 CCGCGG CCGC^GG
8079 CCGCGG
SexAI 164 ACCTGGT A^CC[AT]GGT
5859 ACCTGGT
SmaI 7768 CCCGGG CCC^GGG
8422 CCCGGG
SphI 596 GCATGC GCATG^C
8772 GCATGC
StuI 4775 AGGCCT AGG^CCT
6083 AGGCCT
Tth111I 3344 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
8352 GACGGAGTC
XmaCI 7768 CCCGGG C^CCGGG
8422 CCCGGG
XmaI 7768 CCCGGG C^CCGGG
8422 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApaI ApoI Asp700I AspHI AvaI
AvaII AvrII BalI BanII BbsI
BbvCI BbvI BcgI BciVI BcnI
BfaI BglII BlnI BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspLU11I BspMI BsrDI
BsrGI BsrI BssHII BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRI EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeI NdeII NlaIII NlaIV NsiI
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmnI