##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI Asp718I AspEI AvaI AviII
AvrII BbeI BbrPI BbvCI BcgI
BclI BglI BlnI BlpI Bpu1102I
BsaAI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsoBI BspDI BspEI
BsrFI BssHII BssSI BstBI CelII
Cfr10I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoRV FbaI FseI FspI HaeII
HgaI Hin1I KasI KpnI KspI
MfeI MluI MroI MunI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PvuI RsrII
SacI SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I StuI SwaI
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccII 27 CGCG CG^CG
AclI 1467 AACGTT AA^CGTT
AflII 2795 CTTAAG C^TTAAG
Asp700I 395 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1316 GTGCTC G[AT]GC[AT]^C
AspI 268 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1999 GGATCC G^GATCC
BanII 1019 GAGCCC G[GA]GC[CT]^C
BciVI 3278 GGATAC GTATCC(6/5)
BcnI 2821 CCGGG CC^[GC]GG
BfrI 2795 CTTAAG C^TTAAG
BseRI 103 GAGGAG GAGGAG(10/8)
BsgI 3771 GTGCAG GTGCAG(16/14)
BsiHKAI 1316 GTGCTC G[AT]GC[AT]^C
BsmBI 2269 GAGACG CGTCTC(1/5)
BspHI 192 TCATGA T^CATGA
BsrBI 96 CCGCTC CCGCTC(-3/-3)
Bst1107I 494 GTATAC GTA^TAC
BstAPI 3308 GCAGTTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 27 CGCG CG^CG
BstZ17I 494 GTATAC GTA^TAC
DraII 15 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO109I 15 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT22I 3736 ATGCAT ATGCA^T
HapII 2821 CCGG C^CGG
HindIII 3248 AAGCTT A^AGCTT
HpaII 2821 CCGG C^CGG
HphI 3601 TCACC GGTGA(8/7)
MspI 2821 CCGG C^CGG
MvnI 27 CGCG CG^CG
NciI 2821 CCGGG CC^[GC]GG
NsiI 3736 ATGCAT ATGCA^T
PflFI 268 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 15 GGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 1467 AACGTT AA^CGTT
RcaI 192 TCATGA T^CATGA
SapI 2950 GAAGAGC GCTCTTC(1/4)
SpeI 4093 ACTAGT A^CTAGT
Tth111I 268 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 3586 CCATGTCTTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 395 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 494 GTATAC GT^[AC][GT]AC
1672 GTAGAC
AflIII 1165 ACATGT A^C[GA][CT]GT
2784 ACATGT
AocI 34 CCTCAGG CC^T[ACGT]AGG
2488 CCTGAGG
AseI 3654 ATTAAT AT^TAAT
4076 ATTAAT
AsnI 3654 ATTAAT AT^TAAT
4076 ATTAAT
BalI 1448 TGGCCA TGG^CCA
3870 TGGCCA
BglII 333 AGATCT A^GATCT
664 AGATCT
BsaBI 328 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
699 GATAGAAATC
BsmFI 15 GGGAC GGGAC(10/14)
3450 GTCCC
Bsp1407I 1908 TGTACA T^GTACA
2754 TGTACA
BspLU11I 1165 ACATGT A^CATGT
2784 ACATGT
BspMI 1456 GCAGGT ACCTGC(4/8)
3924 GCAGGT
BsrGI 1908 TGTACA T^GTACA
2754 TGTACA
BstXI 1814 CCAGCACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1985 CCAAAGCAATGG
Bsu36I 34 CCTCAGG CC^T[ACGT]AGG
2488 CCTGAGG
DraI 2916 TTTAAA TTT^AAA
3141 TTTAAA
DraIII 1112 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
2187 CACCAAGTG
EarI 546 CTCTTC CTCTTC(1/4)
2950 GAAGAG
Eco81I 34 CCTCAGG CC^T[ACGT]AGG
2488 CCTGAGG
EcoRI 156 GAATTC G^AATTC
2010 GAATTC
HincII 2476 GTTAAC GT[CT]^[GA]AC
3928 GTTAAC
HindII 2476 GTTAAC GT[CT]^[GA]AC
3928 GTTAAC
HpaI 2476 GTTAAC GTT^AAC
3928 GTTAAC
Ksp632I 546 CTCTTC CTCTTC(1/4)
2950 GAAGAG
MamI 328 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
699 GATAGAAATC
MluNI 1448 TGGCCA TGG^CCA
3870 TGGCCA
MscI 1448 TGGCCA TGG^CCA
3870 TGGCCA
NdeI 3227 CATATG CA^TATG
3407 CATATG
PflMI 871 CCAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2186 CCACCAAGTGG
PshBI 3654 ATTAAT AT^TAAT
4076 ATTAAT
SexAI 3338 ACCTGGT A^CC[AT]GGT
4067 ACCAGGT
SspBI 1908 TGTACA T^GTACA
2754 TGTACA
Van91I 871 CCAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2186 CCACCAAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BanI BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaJI BsiYI BslI BsmAI BsmI
Bsp1286I BsrDI BsrI BssKI BstEII
BstF5I BstNI BstPI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EaeI EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MvaI MwoI NcoI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII