##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII AccIII AcyI AgeI AhdI
Aor51HI ApaI AscI AspEI AviII
BamHI BbeI BbvCI BsaHI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BssHII BssSI BstEII BstPI ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRV FauI FseI
FspI Hin1I HpaI KasI KspI
MluI MroI NaeI NarI NcoI
NgoMIV NotI PacI PinAI PmeI
PvuI RsrII SacII SalI SbfI
SfiI SfoI SgrAI SnaBI SpeI
Sse8387I
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 770 GGTACC G^GTACC
AclI 3591 AACGTT AA^CGTT
Alw44I 1989 GTGCAC G^TGCAC
ApaLI 1989 GTGCAC G^TGCAC
Asp718I 770 GGTACC G^GTACC
AspI 5399 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 8525 CCTAGG C^CTAGG
BalI 46 TGGCCA TGG^CCA
BbrPI 5517 CACGTG CAC^GTG
BcgI 9962 GCAGTCTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 7973 GTATCC GTATCC(6/5)
BglI 1697 GCCAACAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 8525 CCTAGG C^CTAGG
BsrBI 929 GAGCGG CCGCTC(-3/-3)
BsrFI 911 ACCGGC [GA]^CCGG[CT]
BstBI 6864 TTCGAA TT^CGAA
Cfr10I 911 ACCGGC [GA]^CCGG[CT]
DraIII 8472 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 9956 GACATTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
KpnI 770 GGTACC GGTAC^C
MluNI 46 TGGCCA TGG^CCA
MscI 46 TGGCCA TGG^CCA
NheI 1159 GCTAGC G^CTAGC
NruI 1148 TCGCGA TCG^CGA
NspV 6864 TTCGAA TT^CGAA
PflFI 5399 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 5517 CACGTG CAC^GTG
PmlI 5517 CACGTG CAC^GTG
PshAI 2179 GACAATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3591 AACGTT AA^CGTT
SacI 9824 GAGCTC GAGCT^C
SapI 1535 GAAGAGC GCTCTTC(1/4)
SfuI 6864 TTCGAA TT^CGAA
SmaI 1275 CCCGGG CCC^GGG
SphI 2271 GCATGC GCATG^C
StuI 2354 AGGCCT AGG^CCT
SwaI 5550 ATTTAAAT ATTT^AAAT
Tth111I 5399 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1275 CCCGGG C^CCGGG
XmaI 1275 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AflII 6914 CTTAAG C^TTAAG
7510 CTTAAG
AocI 7230 CCTCAGG CC^T[ACGT]AGG
8049 CCTCAGG
BanII 6128 GAGCCC G[GA]GC[CT]^C
9824 GAGCTC
BclI 175 TGATCA T^GATCA
6613 TGATCA
BfrI 6914 CTTAAG C^TTAAG
7510 CTTAAG
BlpI 926 GCTGAGC GC^T[ACGT]AGC
2453 GCTCAGC
Bpu1102I 926 GCTGAGC GC^T[ACGT]AGC
2453 GCTCAGC
BsaAI 4920 CACGTA [CT]AC^GT[GA]
5517 CACGTG
BsaWI 2022 ACCGGA [AT]^CCGG[AT]
6034 TCCGGT
BsmI 5333 GAATGC GAATGC(1/-1)
7315 GAATGC
Bsp1407I 4998 TGTACA T^GTACA
8920 TGTACA
BspHI 1129 TCATGA T^CATGA
1435 TCATGA
BsrGI 4998 TGTACA T^GTACA
8920 TGTACA
Bst1107I 846 GTATAC GTA^TAC
8842 GTATAC
BstZ17I 846 GTATAC GTA^TAC
8842 GTATAC
Bsu36I 7230 CCTCAGG CC^T[ACGT]AGG
8049 CCTCAGG
CelII 926 GCTGAGC GC^T[ACGT]AGC
2453 GCTCAGC
DraII 4839 AGGACCT [GA]G^G[ACGT]CC[CT]
7583 AGGTCCC
Eco81I 7230 CCTCAGG CC^T[ACGT]AGG
8049 CCTCAGG
EcoO109I 4839 AGGACCT [GA]G^G[ACGT]CC[CT]
7583 AGGTCCC
FbaI 175 TGATCA T^GATCA
6613 TGATCA
HaeII 7728 AGCGCC [GA]GCGC^[CT]
8652 AGCGCC
HgaI 367 GCGTC GACGC(5/10)
5420 GCGTC
HincII 5771 GTTGAC GT[CT]^[GA]AC
7477 GTCAAC
HindII 5771 GTTGAC GT[CT]^[GA]AC
7477 GTCAAC
MfeI 719 CAATTG C^AATTG
9690 CAATTG
MunI 719 CAATTG C^AATTG
9690 CAATTG
PaeR7I 6831 CTCGAG C^TCGAG
7833 CTCGAG
PpuMI 4839 AGGACCT [GA]G^G[AT]CC[CT]
7583 AGGTCCC
RcaI 1129 TCATGA T^CATGA
1435 TCATGA
SexAI 5400 ACCAGGT A^CC[AT]GGT
10493 ACCTGGT
SspBI 4998 TGTACA T^GTACA
8920 TGTACA
XhoI 6831 CTCGAG C^TCGAG
7833 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApoI
AseI AsnI Asp700I AspHI AvaI
AvaII BanI BbsI BbvI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaBI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspLU11I BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DsaI
EaeI EarI EcoNI EcoRI EcoRII
EcoT14I EcoT22I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeI NdeII NlaIII NlaIV NsiI
NspI PflMI PleI PshBI PstI
PvuII RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XbaI XcmI XhoII XmnI