################################## # NO CUT ENZYMES ( 49 enzymes) # ################################## AatII AclI AgeI Aor51HI AscI AviII BbeI BbrPI BcgI BsaBI BsiEI BsiWI BspDI BsrBI BssHII Bst1107I BstZ17I ClaI CpoI EagI EclXI Eco47III Eco52I EcoRV FseI FspI KasI KspI MamI MluI NarI NotI NruI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SgrAI SnaBI Sse8387I SwaI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AccIII 10011 TCCGGA T^CCGGA AhdI 9207 GACACATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 9207 GACACATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 10613 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BglII 689 AGATCT A^GATCT BsaAI 9281 CACGTA [CT]AC^GT[GA] BsaI 4048 GGTCTC GGTCTC(1/5) BsaWI 10011 TCCGGA [AT]^CCGG[AT] BseAI 10011 TCCGGA T^CCGGA BsmBI 4649 GAGACG CGTCTC(1/5) BsmI 1380 GAATGC GAATGC(1/-1) BspEI 10011 TCCGGA T^CCGGA BstAPI 7145 GCACGCTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 3669 GGTCACC G^GT[ACGT]ACC BstPI 3669 GGTCACC G^GT[ACGT]ACC DrdI 2275 GACCCTTCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 9207 GACACATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 3669 GGTCACC G^GT[ACGT]ACC HaeII 2325 AGCGCC [GA]GCGC^[CT] MroI 10011 TCCGGA T^CCGGA NdeI 8398 CATATG CA^TATG PacI 1977 TTAATTAA TTAAT^TAA PaeR7I 2432 CTCGAG C^TCGAG PflFI 10613 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 9083 CCATTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SapI 9093 GCTCTTC GCTCTTC(1/4) SexAI 7132 ACCTGGT A^CC[AT]GGT Tth111I 10613 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 9083 CCATTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1949 TCTAGA T^CTAGA XhoI 2432 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### Acc65I 4500 GGTACC G^GTACC 4881 GGTACC AcyI 2986 GACGCC G[GA]^CG[CT]C 10376 GACGCC ApaI 2437 GGGCCC GGGCC^C 10025 GGGCCC Asp718I 4500 GGTACC G^GTACC 4881 GGTACC BamHI 2487 GGATCC G^GATCC 6624 GGATCC BglI 9378 GCCTTAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 10379 GCCAACTAGGC BsaHI 2986 GACGCC G[GA]^CG[CT]C 10376 GACGCC Bsp1407I 7370 TGTACA T^GTACA 10763 TGTACA BsrFI 2781 GCCGGC [GA]^CCGG[CT] 9890 GCCGGC BsrGI 7370 TGTACA T^GTACA 10763 TGTACA BssSI 2510 CTCGTG CACGAG(-5/-1) 9752 CTCGTG BstBI 9008 TTCGAA TT^CGAA 9024 TTCGAA Cfr10I 2781 GCCGGC [GA]^CCGG[CT] 9890 GCCGGC Hin1I 2986 GACGCC G[GA]^CG[CT]C 10376 GACGCC HindIII 1926 AAGCTT A^AGCTT 8911 AAGCTT KpnI 4500 GGTACC GGTAC^C 4881 GGTACC MfeI 181 CAATTG C^AATTG 7859 CAATTG MunI 181 CAATTG C^AATTG 7859 CAATTG NaeI 2781 GCCGGC GCC^GGC 9890 GCCGGC NgoMIV 2781 GCCGGC G^CCGGC 9890 GCCGGC NheI 3978 GCTAGC G^CTAGC 7781 GCTAGC NspV 9008 TTCGAA TT^CGAA 9024 TTCGAA PshAI 7060 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 8961 GACAGCAGTC SfuI 9008 TTCGAA TT^CGAA 9024 TTCGAA SmaI 2491 CCCGGG CCC^GGG 5492 CCCGGG SpeI 291 ACTAGT A^CTAGT 3464 ACTAGT SspBI 7370 TGTACA T^GTACA 10763 TGTACA StuI 1174 AGGCCT AGG^CCT 5739 AGGCCT XcmI 2442 CCAAGGGACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2470 CCACTGGAAGGCTGG XmaCI 2491 CCCGGG C^CCGGG 5492 CCCGGG XmaI 2491 CCCGGG C^CCGGG 5492 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 147 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflII AflIII AluI Alw44I AlwI AlwNI AocI ApaLI ApoI AseI AsnI Asp700I AspHI AvaI AvaII AvrII BalI BanI BanII BbsI BbvCI BbvI BciVI BclI BcnI BfaI BfrI BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspLU11I BspMI BsrDI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRI EcoRII EcoT14I EcoT22I FauI FbaI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NsiI NspI PleI PpuMI PshBI PstI PvuII RcaI RsaI SacI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SphI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI