##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
AatII AclI AgeI Aor51HI AscI
AviII BbeI BbrPI BcgI BsaBI
BsiEI BsiWI BspDI BsrBI BssHII
Bst1107I BstZ17I ClaI CpoI EagI
EclXI Eco47III Eco52I EcoRV FseI
FspI KasI KspI MamI MluI
NarI NotI NruI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SacII SalI SbfI SfiI SfoI
SgrAI SnaBI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AccIII 10011 TCCGGA T^CCGGA
AhdI 9207 GACACATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 9207 GACACATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 10613 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BglII 689 AGATCT A^GATCT
BsaAI 9281 CACGTA [CT]AC^GT[GA]
BsaI 4048 GGTCTC GGTCTC(1/5)
BsaWI 10011 TCCGGA [AT]^CCGG[AT]
BseAI 10011 TCCGGA T^CCGGA
BsmBI 4649 GAGACG CGTCTC(1/5)
BsmI 1380 GAATGC GAATGC(1/-1)
BspEI 10011 TCCGGA T^CCGGA
BstAPI 7145 GCACGCTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3669 GGTCACC G^GT[ACGT]ACC
BstPI 3669 GGTCACC G^GT[ACGT]ACC
DrdI 2275 GACCCTTCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 9207 GACACATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3669 GGTCACC G^GT[ACGT]ACC
HaeII 2325 AGCGCC [GA]GCGC^[CT]
MroI 10011 TCCGGA T^CCGGA
NdeI 8398 CATATG CA^TATG
PacI 1977 TTAATTAA TTAAT^TAA
PaeR7I 2432 CTCGAG C^TCGAG
PflFI 10613 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 9083 CCATTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 9093 GCTCTTC GCTCTTC(1/4)
SexAI 7132 ACCTGGT A^CC[AT]GGT
Tth111I 10613 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 9083 CCATTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1949 TCTAGA T^CTAGA
XhoI 2432 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 4500 GGTACC G^GTACC
4881 GGTACC
AcyI 2986 GACGCC G[GA]^CG[CT]C
10376 GACGCC
ApaI 2437 GGGCCC GGGCC^C
10025 GGGCCC
Asp718I 4500 GGTACC G^GTACC
4881 GGTACC
BamHI 2487 GGATCC G^GATCC
6624 GGATCC
BglI 9378 GCCTTAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
10379 GCCAACTAGGC
BsaHI 2986 GACGCC G[GA]^CG[CT]C
10376 GACGCC
Bsp1407I 7370 TGTACA T^GTACA
10763 TGTACA
BsrFI 2781 GCCGGC [GA]^CCGG[CT]
9890 GCCGGC
BsrGI 7370 TGTACA T^GTACA
10763 TGTACA
BssSI 2510 CTCGTG CACGAG(-5/-1)
9752 CTCGTG
BstBI 9008 TTCGAA TT^CGAA
9024 TTCGAA
Cfr10I 2781 GCCGGC [GA]^CCGG[CT]
9890 GCCGGC
Hin1I 2986 GACGCC G[GA]^CG[CT]C
10376 GACGCC
HindIII 1926 AAGCTT A^AGCTT
8911 AAGCTT
KpnI 4500 GGTACC GGTAC^C
4881 GGTACC
MfeI 181 CAATTG C^AATTG
7859 CAATTG
MunI 181 CAATTG C^AATTG
7859 CAATTG
NaeI 2781 GCCGGC GCC^GGC
9890 GCCGGC
NgoMIV 2781 GCCGGC G^CCGGC
9890 GCCGGC
NheI 3978 GCTAGC G^CTAGC
7781 GCTAGC
NspV 9008 TTCGAA TT^CGAA
9024 TTCGAA
PshAI 7060 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
8961 GACAGCAGTC
SfuI 9008 TTCGAA TT^CGAA
9024 TTCGAA
SmaI 2491 CCCGGG CCC^GGG
5492 CCCGGG
SpeI 291 ACTAGT A^CTAGT
3464 ACTAGT
SspBI 7370 TGTACA T^GTACA
10763 TGTACA
StuI 1174 AGGCCT AGG^CCT
5739 AGGCCT
XcmI 2442 CCAAGGGACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2470 CCACTGGAAGGCTGG
XmaCI 2491 CCCGGG C^CCGGG
5492 CCCGGG
XmaI 2491 CCCGGG C^CCGGG
5492 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 147 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflII AflIII AluI Alw44I AlwI
AlwNI AocI ApaLI ApoI AseI
AsnI Asp700I AspHI AvaI AvaII
AvrII BalI BanI BanII BbsI
BbvCI BbvI BciVI BclI BcnI
BfaI BfrI BlnI BlpI BmyI
BpmI Bpu1102I BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspHI BspLU11I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRI EcoRII EcoT14I EcoT22I FauI
FbaI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NsiI NspI PleI
PpuMI PshBI PstI PvuII RcaI
RsaI SacI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SphI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI